Adaptive changes in global gene expression profile of lung
carcinoma A549 cells acutely exposed to distinct types of AhR
ligands

J 2018

Adaptive changes in global gene expression profile of lung carcinoma A549 cells acutely exposed to distinct types of AhR ligands

PROCHÁZKOVÁ, Jiřina, Simona STRAPÁČOVÁ, Lucie SVRŽKOVÁ, Zdeněk ANDRYSÍK, Martina HYZDALOVA et. al.

Základní údaje

Originální název

Adaptive changes in global gene expression profile of lung carcinoma A549 cells acutely exposed to distinct types of AhR ligands

Autoři

Vydání

Toxicology Letters, CLARE, Elsevier, 2018, 0378-4274

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.499

DOI

http://dx.doi.org/10.1016/j.toxlet.2018.04.024

UT WoS

000433259100018

Klíčová slova anglicky

Aryl hydrocarbon receptor; Lung cancer; Dioxins; Global gene expression profiling

Štítky

RIV ne

Změněno: 28. 5. 2019 17:33, Mgr. Jiřina Procházková, Ph.D.

Anotace

V originále

Exposure to persistent ligands of aryl hydrocarbon receptor (AhR) has been found to cause lung cancer in experimental animals, and lung adenocarcinomas are often associated with enhanced AhR expression and aberrant AhR activation. In order to better understand the action of toxic AhR ligands in lung epithelial cells, we performed global gene expression profiling and analyze TCDD-induced changes in A549 transcriptome, both sensitive and non-sensitive to CH223191 co-treatment. Comparison of our data with results from previously reported microarray and ChIP-seq experiments enabled us to identify candidate genes, which expression status reflects exposure of lung cancer cells to TCDD, and to predict processes, pathways (e.g. ER stress, Wnt/beta-cat, IFN., EGFR/Erbb1), putative TFs (e.g. STAT, AP1, E2F1, TCF4), which may be implicated in adaptive response of lung cells to TCDD-induced AhR activation. Importantly, TCDD-like expression fingerprint of selected genes was observed also in A549 cells exposed acutely to both toxic (benzo[ a] pyrene, benzo[k] fluoranthene) and endogenous AhR ligands (2-(1H-Indol-3-ylcarbonyl)-4-thiazolecarboxylic acid methyl ester and 6-formylindolo [3,2-b] carbazole). Overall, our results suggest novel cellular candidates, which could help to improve monitoring of AhR-dependent transcriptional activity during acute exposure of lung cells to distinct types of environmental pollutants.

Citovat

PROCHÁZKOVÁ, Jiřina, Simona STRAPÁČOVÁ, Lucie SVRŽKOVÁ, Zdeněk ANDRYSÍK, Martina HYZDALOVA, Eva HRUBÁ, Kateřina PĚNČÍKOVÁ, H LIBALOVA, J TOPINKA, Jiří KLÉMA, JM ESPINOSA, Jan VONDRÁČEK a Miroslav MACHALA. Adaptive changes in global gene expression profile of lung carcinoma A549 cells acutely exposed to distinct types of AhR ligands. Toxicology Letters. CLARE: Elsevier, 2018, roč. 292, s. 162-174. ISSN 0378-4274. Dostupné z: https://dx.doi.org/10.1016/j.toxlet.2018.04.024.

@article{1534738,
   author = {Procházková, Jiřina and Strapáčová, Simona and Svržková, Lucie and Andrysík, Zdeněk and Hyzdalova, Martina and Hrubá, Eva and Pěnčíková, Kateřina and Libalova, H and Topinka, J and Kléma, Jiří and Espinosa, JM and Vondráček, Jan and Machala, Miroslav},
   article_location = {CLARE},
   doi = {http://dx.doi.org/10.1016/j.toxlet.2018.04.024},
   keywords = {Aryl hydrocarbon receptor; Lung cancer; Dioxins; Global gene expression profiling},
   language = {eng},
   issn = {0378-4274},
   journal = {Toxicology Letters},
   title = {Adaptive changes in global gene expression profile of lung carcinoma A549 cells acutely exposed to distinct types of AhR ligands},
   url = {http://dx.doi.org/10.1016/j.toxlet.2018.04.024},
   volume = {292},
   year = {2018}
}

TY  - JOUR
ID  - 1534738
AU  - Procházková, Jiřina - Strapáčová, Simona - Svržková, Lucie - Andrysík, Zdeněk - Hyzdalova, Martina - Hrubá, Eva - Pěnčíková, Kateřina - Libalova, H - Topinka, J - Kléma, Jiří - Espinosa, JM - Vondráček, Jan - Machala, Miroslav
PY  - 2018
TI  - Adaptive changes in global gene expression profile of lung carcinoma A549 cells acutely exposed to distinct types of AhR ligands
JF  - Toxicology Letters
VL  - 292
SP  - 162-174
EP  - 162-174
PB  - Elsevier
SN  - 03784274
KW  - Aryl hydrocarbon receptor
KW  - Lung cancer
KW  - Dioxins
KW  - Global gene expression profiling
UR  - http://dx.doi.org/10.1016/j.toxlet.2018.04.024
L2  - http://dx.doi.org/10.1016/j.toxlet.2018.04.024
N2  - Exposure to persistent ligands of aryl hydrocarbon receptor (AhR) has been found to cause lung cancer in experimental animals, and lung adenocarcinomas are often associated with enhanced AhR expression and aberrant AhR activation. In order to better understand the action of toxic AhR ligands in lung epithelial cells, we performed global gene expression profiling and analyze TCDD-induced changes in A549 transcriptome, both sensitive and non-sensitive to CH223191 co-treatment. Comparison of our data with results from previously reported microarray and ChIP-seq experiments enabled us to identify candidate genes, which expression status reflects exposure of lung cancer cells to TCDD, and to predict processes, pathways (e.g. ER stress, Wnt/beta-cat, IFN., EGFR/Erbb1), putative TFs (e.g. STAT, AP1, E2F1, TCF4), which may be implicated in adaptive response of lung cells to TCDD-induced AhR activation. Importantly, TCDD-like expression fingerprint of selected genes was observed also in A549 cells exposed acutely to both toxic (benzo[ a] pyrene, benzo[k] fluoranthene) and endogenous AhR ligands (2-(1H-Indol-3-ylcarbonyl)-4-thiazolecarboxylic acid methyl ester and 6-formylindolo [3,2-b] carbazole). Overall, our results suggest novel cellular candidates, which could help to improve monitoring of AhR-dependent transcriptional activity during acute exposure of lung cells to distinct types of environmental pollutants.
ER  -

PROCHÁZKOVÁ, Jiřina, Simona STRAPÁČOVÁ, Lucie SVRŽKOVÁ, Zdeněk ANDRYSÍK, Martina HYZDALOVA, Eva HRUBÁ, Kateřina PĚNČÍKOVÁ, H LIBALOVA, J TOPINKA, Jiří KLÉMA, JM ESPINOSA, Jan VONDRÁČEK a Miroslav MACHALA. Adaptive changes in global gene expression profile of lung carcinoma A549 cells acutely exposed to distinct types of AhR ligands. \textit{Toxicology Letters}. CLARE: Elsevier, 2018, roč.~292, s.~162-174. ISSN~0378-4274. Dostupné z: https://dx.doi.org/10.1016/j.toxlet.2018.04.024.

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