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@article{1538516,
author = {Kubíčková, Barbara and Labohá, Petra and Hildebrandt, JanandPeter and Hilscherová, Klára and Babica, Pavel},
article_location = {OXFORD},
article_number = {April},
doi = {http://dx.doi.org/10.1016/j.chemosphere.2018.12.157},
keywords = {PROTEIN-SYNTHESIS INHIBITION; BLUE-GREEN-ALGAE; RECREATIONAL WATER; LYNGBYA-MAJUSCULA; CYANOBACTERIA; TOXICITY; RACIBORSKII; BLOOMS; CYANOTOXINS; EXPRESSION},
language = {eng},
issn = {0045-6535},
journal = {Chemosphere},
title = {Effects of cylindrospermopsin on cultured immortalized human airway epithelial cells},
url = {https://www.sciencedirect.com/science/article/pii/S0045653518324925?via%3Dihub},
volume = {220},
year = {2019}
}
TY - JOUR
ID - 1538516
AU - Kubíčková, Barbara - Labohá, Petra - Hildebrandt, Jan-Peter - Hilscherová, Klára - Babica, Pavel
PY - 2019
TI - Effects of cylindrospermopsin on cultured immortalized human airway epithelial cells
JF - Chemosphere
VL - 220
IS - April
SP - 620-628
EP - 620-628
PB - PERGAMON-ELSEVIER SCIENCE LTD
SN - 00456535
KW - PROTEIN-SYNTHESIS INHIBITION
KW - BLUE-GREEN-ALGAE
KW - RECREATIONAL WATER
KW - LYNGBYA-MAJUSCULA
KW - CYANOBACTERIA
KW - TOXICITY
KW - RACIBORSKII
KW - BLOOMS
KW - CYANOTOXINS
KW - EXPRESSION
UR - https://www.sciencedirect.com/science/article/pii/S0045653518324925?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0045653518324925?via%3Dihub
N2 - Anthropogenic eutrophication of freshwater bodies increases the occurrence of toxic cyanobacterial blooms. The cyanobacterial toxin cylindrospermopsin (CYN) is detected in the environment with increasing frequency, driving the scientific effort to assess emerging health risks from CYN-producing blooms. Oral exposure to CYN results primarily in hepatotoxicity. Nevertheless, extrahepatic manifestations of CYN toxicity have been reported. Furthermore, cyanotoxins have been detected in aerosols and dust particles, suggesting potential toxic effects in the respiratory tract. To assess the susceptibility of airway epithelia towards cyanotoxins, monolayers of immortalized human bronchial epithelial cells HBE1 and 16HBE14o- were exposed to a concentration range of 0.1-10 mu M CYN. Cytotoxic endpoints were assessed as morphologic alterations, resazurin reduction capacity, esterase activity, neutral red uptake, and by impedimetric real-time cell analysis. Depending on the endpoint assessed, EC50 values ranged between 0.7 and 1.8 mu M (HBE1) and 1.6-4.8 mu M (16HBE14o-). To evaluate alterations of other cellular events by subcytotoxic concentration of CYN (1 mu M), phosphorylation of mitogen-activated protein kinases ERK and p38 was determined. Only a slight increase in p38 phosphorylation was induced by CYN in HBE1 cell line after 48 h, while activities of both ERK1/2 and p38 gradually and significantly increased in 16HBE14o- cells during 8-48 h exposure. This study suggests possible hazards of inhalation CYN exposures, which may severely impact the integrity of airway epithelia and epithelial cell signaling. Further research of CYN-induced toxicity and underlying mechanisms is needed, as well as more data on environmental concentrations of cyanotoxins in aerosols for exposure assessment. (C) 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
ER -
KUBÍČKOVÁ, Barbara, Petra LABOHÁ, Jan-Peter HILDEBRANDT, Klára HILSCHEROVÁ a Pavel BABICA. Effects of cylindrospermopsin on cultured immortalized human airway epithelial cells. \textit{Chemosphere}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2019, roč.~220, April, s.~620-628. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2018.12.157.
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