Effects of cylindrospermopsin on cultured immortalized human
airway epithelial cells

J 2019

Effects of cylindrospermopsin on cultured immortalized human airway epithelial cells

KUBÍČKOVÁ, Barbara; Petra LABOHÁ; Jan-Peter HILDEBRANDT; Klára HILSCHEROVÁ; Pavel BABICA et al.

Základní údaje

Originální název

Effects of cylindrospermopsin on cultured immortalized human airway epithelial cells

Autoři

KUBÍČKOVÁ, Barbara; Petra LABOHÁ; Jan-Peter HILDEBRANDT; Klára HILSCHEROVÁ a Pavel BABICA

Vydání

Chemosphere, OXFORD, PERGAMON-ELSEVIER SCIENCE LTD, 2019, 0045-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 5.778

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00107451

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

PROTEIN-SYNTHESIS INHIBITION; BLUE-GREEN-ALGAE; RECREATIONAL WATER; LYNGBYA-MAJUSCULA; CYANOBACTERIA; TOXICITY; RACIBORSKII; BLOOMS; CYANOTOXINS; EXPRESSION

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 3. 2020 16:29, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Anthropogenic eutrophication of freshwater bodies increases the occurrence of toxic cyanobacterial blooms. The cyanobacterial toxin cylindrospermopsin (CYN) is detected in the environment with increasing frequency, driving the scientific effort to assess emerging health risks from CYN-producing blooms. Oral exposure to CYN results primarily in hepatotoxicity. Nevertheless, extrahepatic manifestations of CYN toxicity have been reported. Furthermore, cyanotoxins have been detected in aerosols and dust particles, suggesting potential toxic effects in the respiratory tract. To assess the susceptibility of airway epithelia towards cyanotoxins, monolayers of immortalized human bronchial epithelial cells HBE1 and 16HBE14o- were exposed to a concentration range of 0.1-10 mu M CYN. Cytotoxic endpoints were assessed as morphologic alterations, resazurin reduction capacity, esterase activity, neutral red uptake, and by impedimetric real-time cell analysis. Depending on the endpoint assessed, EC50 values ranged between 0.7 and 1.8 mu M (HBE1) and 1.6-4.8 mu M (16HBE14o-). To evaluate alterations of other cellular events by subcytotoxic concentration of CYN (1 mu M), phosphorylation of mitogen-activated protein kinases ERK and p38 was determined. Only a slight increase in p38 phosphorylation was induced by CYN in HBE1 cell line after 48 h, while activities of both ERK1/2 and p38 gradually and significantly increased in 16HBE14o- cells during 8-48 h exposure. This study suggests possible hazards of inhalation CYN exposures, which may severely impact the integrity of airway epithelia and epithelial cell signaling. Further research of CYN-induced toxicity and underlying mechanisms is needed, as well as more data on environmental concentrations of cyanotoxins in aerosols for exposure assessment. (C) 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Návaznosti

EF16_013/0001761, projekt VaV

Název: RECETOX RI

GJ17-25279Y, projekt VaV

Název: Zdravotní rizika spojená s inhalací sinic

Investor: Grantová agentura ČR, Zdravotní rizika spojená s inhalací sinic

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

722493, interní kód MU

Název: Natural Toxins and Drinking Water Quality - From Source to Tap (Akronym: NaToxAq)

Investor: Evropská unie, Natural Toxins and Drinking Water Quality - From Source to Tap, MSCA Marie Skłodowska-Curie Actions (Excellent Science)

Citovat

KUBÍČKOVÁ, Barbara; Petra LABOHÁ; Jan-Peter HILDEBRANDT; Klára HILSCHEROVÁ a Pavel BABICA. Effects of cylindrospermopsin on cultured immortalized human airway epithelial cells. Chemosphere. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2019, roč. 220, April, s. 620-628. ISSN 0045-6535. Dostupné z: https://doi.org/10.1016/j.chemosphere.2018.12.157.

@article{1538516,
   author = {Kubíčková, Barbara and Labohá, Petra and Hildebrandt, JanandPeter and Hilscherová, Klára and Babica, Pavel},
   article_location = {OXFORD},
   article_number = {April},
   doi = {https://doi.org/10.1016/j.chemosphere.2018.12.157},
   keywords = {PROTEIN-SYNTHESIS INHIBITION; BLUE-GREEN-ALGAE; RECREATIONAL WATER; LYNGBYA-MAJUSCULA; CYANOBACTERIA; TOXICITY; RACIBORSKII; BLOOMS; CYANOTOXINS; EXPRESSION},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Effects of cylindrospermopsin on cultured immortalized human airway epithelial cells},
   url = {https://www.sciencedirect.com/science/article/pii/S0045653518324925?via%3Dihub},
   volume = {220},
   year = {2019}
}

TY  - JOUR
ID  - 1538516
AU  - Kubíčková, Barbara - Labohá, Petra - Hildebrandt, Jan-Peter - Hilscherová, Klára - Babica, Pavel
PY  - 2019
TI  - Effects of cylindrospermopsin on cultured immortalized human airway epithelial cells
JF  - Chemosphere
VL  - 220
IS  - April
SP  - 620-628
EP  - 620-628
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00456535
KW  - PROTEIN-SYNTHESIS INHIBITION
KW  - BLUE-GREEN-ALGAE
KW  - RECREATIONAL WATER
KW  - LYNGBYA-MAJUSCULA
KW  - CYANOBACTERIA
KW  - TOXICITY
KW  - RACIBORSKII
KW  - BLOOMS
KW  - CYANOTOXINS
KW  - EXPRESSION
UR  - https://www.sciencedirect.com/science/article/pii/S0045653518324925?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0045653518324925?via%3Dihub
N2  - Anthropogenic eutrophication of freshwater bodies increases the occurrence of toxic cyanobacterial blooms. The cyanobacterial toxin cylindrospermopsin (CYN) is detected in the environment with increasing frequency, driving the scientific effort to assess emerging health risks from CYN-producing blooms. Oral exposure to CYN results primarily in hepatotoxicity. Nevertheless, extrahepatic manifestations of CYN toxicity have been reported. Furthermore, cyanotoxins have been detected in aerosols and dust particles, suggesting potential toxic effects in the respiratory tract. To assess the susceptibility of airway epithelia towards cyanotoxins, monolayers of immortalized human bronchial epithelial cells HBE1 and 16HBE14o- were exposed to a concentration range of 0.1-10 mu M CYN. Cytotoxic endpoints were assessed as morphologic alterations, resazurin reduction capacity, esterase activity, neutral red uptake, and by impedimetric real-time cell analysis. Depending on the endpoint assessed, EC50 values ranged between 0.7 and 1.8 mu M (HBE1) and 1.6-4.8 mu M (16HBE14o-). To evaluate alterations of other cellular events by subcytotoxic concentration of CYN (1 mu M), phosphorylation of mitogen-activated protein kinases ERK and p38 was determined. Only a slight increase in p38 phosphorylation was induced by CYN in HBE1 cell line after 48 h, while activities of both ERK1/2 and p38 gradually and significantly increased in 16HBE14o- cells during 8-48 h exposure. This study suggests possible hazards of inhalation CYN exposures, which may severely impact the integrity of airway epithelia and epithelial cell signaling. Further research of CYN-induced toxicity and underlying mechanisms is needed, as well as more data on environmental concentrations of cyanotoxins in aerosols for exposure assessment. (C) 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
ER  -

KUBÍČKOVÁ, Barbara; Petra LABOHÁ; Jan-Peter HILDEBRANDT; Klára HILSCHEROVÁ a Pavel BABICA. Effects of cylindrospermopsin on cultured immortalized human airway epithelial cells. \textit{Chemosphere}. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD, 2019, roč.~220, April, s.~620-628. ISSN~0045-6535. Dostupné z: https://doi.org/10.1016/j.chemosphere.2018.12.157.

Přehled o publikaci