The elicitin -cryptogein's activity in tomato is mediated by
jasmonic acid and ethylene signalling pathways independently of
elicitin-sterol interactions

J 2019

The elicitin -cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions

STARÝ, Tomáš; Pavla ŠATKOVÁ; Jana PITERKOVA; Barbora MIESLEROVA; Lenka LUHOVÁ et al.

Základní údaje

Originální název

The elicitin -cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions

Autoři

STARÝ, Tomáš; Pavla ŠATKOVÁ; Jana PITERKOVA; Barbora MIESLEROVA; Lenka LUHOVÁ; Jaromír MIKULIK; Tomáš KAŠPAROVSKÝ; Marek PETŘIVALSKÝ a Jan LOCHMAN

Vydání

Planta, New York, Springer, 2019, 0032-0935

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 3.390

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00107459

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Defence-related genes; Elicitins; Pseudoidium neolycopersici; Resistance; Signalling; Sterol binding

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 3. 2020 16:34, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Main conclusionThe level of resistance induced in different tomato genotypes after -CRY treatment correlated with the upregulation of defence genes, but not sterol binding and involved ethylene and jasmonic acid signalling.Elicitins, a family of small proteins secreted by Phytophthora and Pythium spp., are the most well-known microbe-associated molecular patterns of oomycetes, a lineage of fungus-like organisms that include many economically significant crop pathogens. The responses of tomato plants to elicitin INF1 produced by Phytophthora infestans have been studied extensively. Here, we present studies on the responses of three tomato genotypes to -cryptogein (-CRY), a potent elicitin secreted by Phytophthora cryptogea that induces hypersensitive response (HR) cell death in tobacco plants and confers greater resistance to oomycete infection than acidic elicitins like INF1. We also studied -CRY mutants impaired in sterol binding (Val84Phe) and interaction with the binding site on tobacco plasma membrane (Leu41Phe), because sterol binding was suggested to be important in INF1-induced resistance. Treatment with -CRY or the Val84Phe mutant induced resistance to powdery mildew caused by the pathogen Pseudoidium neolycopersici, but not the HR cell death observed in tobacco and potato plants. The level of resistance induced in different tomato genotypes correlated with the upregulation of defence genes including defensins, -1,3-glucanases, heveins, chitinases, osmotins, and PR1 proteins. Treatment with the Leu41Phe mutant did not induce this upregulation, suggesting similar elicitin recognition in tomato and tobacco. However, here -CRY activated ethylene and jasmonic acid signalling, but not salicylic acid signalling, demonstrating that elicitins activate different downstream signalling processes in different plant species. This could potentially be exploited to enhance the resistance of Phytophthora-susceptible crops.

Návaznosti

GAP501/12/0590, projekt VaV

Název: Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

Investor: Grantová agentura ČR, Charakterizace procesů zapojených v indukci resistence rostlin na patogeny s využitím elicitinů se změněnou schopností vyvolávat obranné reakce

Citovat

STARÝ, Tomáš; Pavla ŠATKOVÁ; Jana PITERKOVA; Barbora MIESLEROVA; Lenka LUHOVÁ; Jaromír MIKULIK; Tomáš KAŠPAROVSKÝ; Marek PETŘIVALSKÝ a Jan LOCHMAN. The elicitin -cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions. Planta. New York: Springer, 2019, roč. 249, č. 3, s. 739-749. ISSN 0032-0935. Dostupné z: https://doi.org/10.1007/s00425-018-3036-1.

@article{1539480,
   author = {Starý, Tomáš and Šatková, Pavla and Piterkova, Jana and Mieslerova, Barbora and Luhová, Lenka and Mikulik, Jaromír and Kašparovský, Tomáš and Petřivalský, Marek and Lochman, Jan},
   article_location = {New York},
   article_number = {3},
   doi = {https://doi.org/10.1007/s00425-018-3036-1},
   keywords = {Defence-related genes; Elicitins; Pseudoidium neolycopersici; Resistance; Signalling; Sterol binding},
   language = {eng},
   issn = {0032-0935},
   journal = {Planta},
   title = {The elicitin -cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0434.2008.01489.x},
   volume = {249},
   year = {2019}
}

TY  - JOUR
ID  - 1539480
AU  - Starý, Tomáš - Šatková, Pavla - Piterkova, Jana - Mieslerova, Barbora - Luhová, Lenka - Mikulik, Jaromír - Kašparovský, Tomáš - Petřivalský, Marek - Lochman, Jan
PY  - 2019
TI  - The elicitin -cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions
JF  - Planta
VL  - 249
IS  - 3
SP  - 739-749
EP  - 739-749
PB  - Springer
SN  - 00320935
KW  - Defence-related genes
KW  - Elicitins
KW  - Pseudoidium neolycopersici
KW  - Resistance
KW  - Signalling
KW  - Sterol binding
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0434.2008.01489.x
L2  - https://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0434.2008.01489.x
N2  - Main conclusionThe level of resistance induced in different tomato genotypes after -CRY treatment correlated with the upregulation of defence genes, but not sterol binding and involved ethylene and jasmonic acid signalling.Elicitins, a family of small proteins secreted by Phytophthora and Pythium spp., are the most well-known microbe-associated molecular patterns of oomycetes, a lineage of fungus-like organisms that include many economically significant crop pathogens. The responses of tomato plants to elicitin INF1 produced by Phytophthora infestans have been studied extensively. Here, we present studies on the responses of three tomato genotypes to -cryptogein (-CRY), a potent elicitin secreted by Phytophthora cryptogea that induces hypersensitive response (HR) cell death in tobacco plants and confers greater resistance to oomycete infection than acidic elicitins like INF1. We also studied -CRY mutants impaired in sterol binding (Val84Phe) and interaction with the binding site on tobacco plasma membrane (Leu41Phe), because sterol binding was suggested to be important in INF1-induced resistance. Treatment with -CRY or the Val84Phe mutant induced resistance to powdery mildew caused by the pathogen Pseudoidium neolycopersici, but not the HR cell death observed in tobacco and potato plants. The level of resistance induced in different tomato genotypes correlated with the upregulation of defence genes including defensins, -1,3-glucanases, heveins, chitinases, osmotins, and PR1 proteins. Treatment with the Leu41Phe mutant did not induce this upregulation, suggesting similar elicitin recognition in tomato and tobacco. However, here -CRY activated ethylene and jasmonic acid signalling, but not salicylic acid signalling, demonstrating that elicitins activate different downstream signalling processes in different plant species. This could potentially be exploited to enhance the resistance of Phytophthora-susceptible crops.
ER  -

STARÝ, Tomáš; Pavla ŠATKOVÁ; Jana PITERKOVA; Barbora MIESLEROVA; Lenka LUHOVÁ; Jaromír MIKULIK; Tomáš KAŠPAROVSKÝ; Marek PETŘIVALSKÝ a Jan LOCHMAN. The elicitin -cryptogein's activity in tomato is mediated by jasmonic acid and ethylene signalling pathways independently of elicitin-sterol interactions. \textit{Planta}. New York: Springer, 2019, roč.~249, č.~3, s.~739-749. ISSN~0032-0935. Dostupné z: https://doi.org/10.1007/s00425-018-3036-1.

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