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@article{1545943,
author = {Sedláček, Vojtěch and Kučera, Igor},
article_location = {Hoboken},
article_number = {7},
doi = {http://dx.doi.org/10.1002/1873-3468.13359},
keywords = {flavoprotein; antioxidant enzyme; chromate reductase; superoxide reductase},
language = {eng},
issn = {0014-5793},
journal = {FEBS Letters},
title = {Arginine 95 is important for recruiting superoxide to the active site of the FerB flavoenzyme of Paracoccus denitrificans},
url = {https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.13359},
volume = {593},
year = {2019}
}
TY - JOUR
ID - 1545943
AU - Sedláček, Vojtěch - Kučera, Igor
PY - 2019
TI - Arginine 95 is important for recruiting superoxide to the active site of the FerB flavoenzyme of Paracoccus denitrificans
JF - FEBS Letters
VL - 593
IS - 7
SP - 697-702
EP - 697-702
PB - Wiley
SN - 00145793
KW - flavoprotein
KW - antioxidant enzyme
KW - chromate reductase
KW - superoxide reductase
UR - https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.13359
L2 - https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.13359
N2 - Ferric reductase B (FerB) is a flavin mononucleotide (FMN)containing NAD(P)H:acceptor oxidoreductase structurally close to the Gluconacetobacter hansenii chromate reductase (ChrR). The crystal structure of ChrR was previously determined with a chloride bound proximal to FMN in the vicinity of Arg101, and the authors suggested that the anionic electron acceptors, chromate and uranyl tricarbonate, bind similarly. Here, we identify the corresponding arginine residue in FerB (Arg95) as being important for the reaction of FerB with superoxide. Four mutants at position 95 were prepared and found kinetically to have impaired capacity for superoxide binding. Stopped flow data for the flavin cofactor showed that the oxidative step is rate limiting for catalytic turnover. The findings are consistent with a role for FerB as a superoxide scavenging contributor.
ER -
SEDLÁČEK, Vojtěch a Igor KUČERA. Arginine 95 is important for recruiting superoxide to the active site of the FerB flavoenzyme of Paracoccus denitrificans. \textit{FEBS Letters}. Hoboken: Wiley, 2019, roč.~593, č.~7, s.~697-702. ISSN~0014-5793. Dostupné z: https://dx.doi.org/10.1002/1873-3468.13359.
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