Application of CD spectroscopy in analytical chemistry

D 2019

Application of CD spectroscopy in analytical chemistry

VACULÍKOVÁ, Anna; Marta FARKOVÁ a Přemysl LUBAL

Základní údaje

Originální název

Application of CD spectroscopy in analytical chemistry

Autoři

VACULÍKOVÁ, Anna; Marta FARKOVÁ a Přemysl LUBAL

Vydání

1st edition. Brno, XIX. Workshop of Biophysical Chemists and Electrochemists, s. 61-61, 2019

Nakladatel

Masaryk University

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

paměťový nosič (CD, DVD, flash disk)

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-9309-6

Klíčová slova česky

spectroscopy; circular dichroism; tryptophan

Změněno: 27. 7. 2019 16:38, RNDr. Marta Farková, CSc.

Anotace

V originále

Many biological compounds exhibit optical activity, which is due to asymmetric carbon on which four different substituents are attached. This configuration leads to two structurally different forms of one substance, when both forms have the ability to rotate the plane of polarized light, one turns to the left side (L-) and the second one to the right one (D-). This phenomena can be monitored by circular dichroism (CD) spectroscopy when the ellipticity is measured in the region of absorption band and this parameter can be used for structural analysis as well as for quantitative determination. However, if L- and D- forms are present in solution in ratio 1:1, i.e. racemic optically inactive mixture, they does not exhibit any rotatation. In our case, the L- and D- forms of tryptophan in aqueous solution were measured. The spectroscopic measurements were done at Jasco J-810 spectropolarimeter (Jasco, Japan) in the wavelength range of 200-300 nm. CD-spectra of pure L- and D-tryptophan and their racemic mixture are presented. It was demonstrated, that CD spectroscopy can be employed for quantitative analysis and determination of purity of chiral aminoacid (Tryptophan).

Návaznosti

MUNI/A/1359/2018, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

VACULÍKOVÁ, Anna; Marta FARKOVÁ a Přemysl LUBAL. Application of CD spectroscopy in analytical chemistry. In Libuše Trnková. XIX. Workshop of Biophysical Chemists and Electrochemists. 1st edition. Brno: Masaryk University, 2019, s. 61. ISBN 978-80-210-9309-6.

@inproceedings{1547160,
   author = {Vaculíková, Anna and Farková, Marta and Lubal, Přemysl},
   address = {Brno},
   booktitle = {XIX. Workshop of Biophysical Chemists and Electrochemists},
   edition = {1st edition},
   editor = {Libuše Trnková},
   howpublished = {paměťový nosič},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-9309-6},
   pages = {61-61},
   publisher = {Masaryk University},
   title = {Application of CD spectroscopy in analytical chemistry},
   year = {2019}
}

TY  - CONF
ID  - 1547160
AU  - Vaculíková, Anna - Farková, Marta - Lubal, Přemysl
PY  - 2019
TI  - Application of CD spectroscopy in analytical chemistry
PB  - Masaryk University
CY  - Brno
SN  - 9788021093096
N2  - Many biological compounds exhibit optical activity, which is due to asymmetric carbon on which four different substituents are attached. This configuration leads to two structurally different forms of one substance, when both forms have the ability to rotate the plane of polarized light, one turns to the left side (L-) and the second one to the right one (D-). This phenomena can be monitored by circular dichroism (CD) spectroscopy when the ellipticity is measured in the region of absorption band and this parameter can be used for structural analysis as well as for quantitative determination. However, if L- and D- forms are present in solution in ratio 1:1, i.e. racemic optically inactive mixture, they does not exhibit any rotatation. In our case, the L- and D- forms of tryptophan in aqueous solution were measured. The spectroscopic measurements were done at Jasco J-810 spectropolarimeter (Jasco, Japan) in the wavelength range of 200-300 nm. CD-spectra of pure L- and D-tryptophan and their racemic mixture are presented. It was demonstrated, that CD spectroscopy can be employed for quantitative analysis and determination of purity of chiral aminoacid (Tryptophan).
ER  -

VACULÍKOVÁ, Anna; Marta FARKOVÁ a Přemysl LUBAL. Application of CD spectroscopy in analytical chemistry. In Libuše Trnková. \textit{XIX. Workshop of Biophysical Chemists and Electrochemists}. 1st edition. Brno: Masaryk University, 2019, s.~61. ISBN~978-80-210-9309-6.

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