Multicomponent Yeast Two-Hybrid System: Applications to Study
Protein-Protein Interactions in SMC Complexes.

C 2019

Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.

PALEČEK, Jan; Lucie VONDROVÁ; Kateřina ZÁBRADY a Jakub OTOČKA

Základní údaje

Originální název

Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.

Autoři

PALEČEK, Jan; Lucie VONDROVÁ; Kateřina ZÁBRADY a Jakub OTOČKA

Vydání

New York, NY, Methods in Molecular Biology, od s. 79-90, 12 s. SMC Complexes, vol 2004. 2019

Nakladatel

Springer Nature

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10609 Biochemical research methods

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00107533

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-4939-9519-6

Klíčová slova anglicky

KITE proteins; Kleisin bridge; Multicomponent yeast two-hybrid (Y2H) system; Non-SMC Element (Nse) subunits; Protein–protein interactions; SMC5/6; Structural maintenance of chromosomes (SMC) complexes

Štítky

rivok, topvydavatel

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 4. 2020 21:04, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.

Návaznosti

GA18-02067S, projekt VaV

Název: Úloha Nse1/Nse3/Nse4, E3-ubikvitin ligásy, ve stabilitě genomu

Investor: Grantová agentura ČR, Úloha Nse1/Nse3/Nse4, E3-ubikvitin ligásy, ve stabilitě genomu

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

PALEČEK, Jan; Lucie VONDROVÁ; Kateřina ZÁBRADY a Jakub OTOČKA. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. In Badrinarayanan A. (eds). Methods in Molecular Biology. New York, NY: Springer Nature, 2019, s. 79-90. SMC Complexes, vol 2004. ISBN 978-1-4939-9519-6. Dostupné z: https://doi.org/10.1007/978-1-4939-9520-2_7.

@inbook{1548183,
   author = {Paleček, Jan and Vondrová, Lucie and Zábrady, Kateřina and Otočka, Jakub},
   address = {New York, NY},
   booktitle = {Methods in Molecular Biology},
   doi = {https://doi.org/10.1007/978-1-4939-9520-2_7},
   editor = {Badrinarayanan A. (eds)},
   keywords = {KITE proteins; Kleisin bridge; Multicomponent yeast two-hybrid (Y2H) system; Non-SMC Element (Nse) subunits; Protein–protein interactions; SMC5/6; Structural maintenance of chromosomes (SMC) complexes},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York, NY},
   isbn = {978-1-4939-9519-6},
   pages = {79-90},
   publisher = {Springer Nature},
   title = {Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.},
   url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7},
   year = {2019}
}

TY  - CHAP
ID  - 1548183
AU  - Paleček, Jan - Vondrová, Lucie - Zábrady, Kateřina - Otočka, Jakub
PY  - 2019
TI  - Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes.
VL  - SMC Complexes, vol 2004.
PB  - Springer Nature
CY  - New York, NY
SN  - 9781493995196
KW  - KITE proteins
KW  - Kleisin bridge
KW  - Multicomponent yeast two-hybrid (Y2H) system
KW  - Non-SMC Element (Nse) subunits
KW  - Protein–protein interactions
KW  - SMC5/6
KW  - Structural maintenance of chromosomes (SMC) complexes
UR  - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7
L2  - https://link.springer.com/protocol/10.1007%2F978-1-4939-9520-2_7
N2  - Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.
ER  -

PALEČEK, Jan; Lucie VONDROVÁ; Kateřina ZÁBRADY a Jakub OTOČKA. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes. In Badrinarayanan A. (eds). \textit{Methods in Molecular Biology}. New York, NY: Springer Nature, 2019, s.~79-90. SMC Complexes, vol 2004. ISBN~978-1-4939-9519-6. Dostupné z: https://doi.org/10.1007/978-1-4939-9520-2\_{}7.

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