Photorhabdus lectins disrupt the activity of insect and human immune system

DOBEŠ, Pavel, Eva FUJDIAROVÁ, Josef HOUSER, Gita JANČAŘÍKOVÁ, Pavel HYRŠL a Michaela WIMMEROVÁ. Photorhabdus lectins disrupt the activity of insect and human immune system. Online. In International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests”. 2019, [citováno 2024-04-24]

Základní údaje

• Originální název: Photorhabdus lectins disrupt the activity of insect and human immune system
• Autoři: DOBEŠ, Pavel (203 Česká republika, domácí), Eva FUJDIAROVÁ (203 Česká republika, domácí), Josef HOUSER (203 Česká republika, domácí), Gita JANČAŘÍKOVÁ (203 Česká republika, domácí), Pavel HYRŠL (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí)
• Vydání: International Congress on Invertebrate Pathology and Microbial Control & 52nd Annual Meeting of the Society for Invertebrate Pathology & 17th Meeting of the IOBC‐WPRS Working Group “Microbial and Nematode Control of Invertebrate Pests” 2019.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 10608 Biochemistry and molecular biology
• Stát vydavatele: Španělsko
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/19:00107542
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova česky: Photorhabdus; lektin; imunita; melanizace; kyslíkové radikály
• Klíčová slova anglicky: Photorhabdus; lectin; immunity; melanisation; reactive oxygen species
• Příznaky: Mezinárodní význam

Anotace

Bacteria of the genus Photorhabdus are known as potent entomopathogens that produce a variety of toxins, proteases and other virulence factors to overcome host immune system and successfully establish the infection. Although lectins with their carbohydrate-binding abilities are not the typical example of molecules causing direct damage to host organism, they are indispensable in the processes such as attachment to cells, immunoevasion and immunosuppression. Bacteria of the genus Photorhabdus are not an exception as they produce lectins that could help them to interact with nematode symbionts, other bacteria or host immune system. Recently, we focused on lectins produced by P. laumondii (formerly classified as P. luminescens subsp. laumondii) that are not only able to bind to insect haemocytes, but also to disturb cellular and humoral immune response. Lectin treatment of insect haemolymph induced melanisation catalysed by phenoloxidase; this increase was not observed when we used the lectin pre-treated with saccharides selected according to its specificity. Although, P. laumondii is not considered to be human pathogen (unlike closely related P. asymbiotica), its lectin is able to inhibit production of reactive oxygen species in human blood induced by neutrophil activator zymosan A, whereas it is not able to supress the action of other activators such as phorbol 12-myristate 13-acetate or fMLF. Our results suggest that the lectin interferes with Toll-like receptor 2 and thus can impair the production of reactive oxygen species by host phagocytes. The work was supported by the Czech Science Foundation (grants no. 17-03253S and 18-18964S).

Anotace česky

Bacteria of the genus Photorhabdus are known as potent entomopathogens that produce a variety of toxins, proteases and other virulence factors to overcome host immune system and successfully establish the infection. Although lectins with their carbohydrate-binding abilities are not the typical example of molecules causing direct damage to host organism, they are indispensable in the processes such as attachment to cells, immunoevasion and immunosuppression. Bacteria of the genus Photorhabdus are not an exception as they produce lectins that could help them to interact with nematode symbionts, other bacteria or host immune system. Recently, we focused on lectins produced by P. laumondii (formerly classified as P. luminescens subsp. laumondii) that are not only able to bind to insect haemocytes, but also to disturb cellular and humoral immune response. Lectin treatment of insect haemolymph induced melanisation catalysed by phenoloxidase; this increase was not observed when we used the lectin pre-treated with saccharides selected according to its specificity. Although, P. laumondii is not considered to be human pathogen (unlike closely related P. asymbiotica), its lectin is able to inhibit production of reactive oxygen species in human blood induced by neutrophil activator zymosan A, whereas it is not able to supress the action of other activators such as phorbol 12-myristate 13-acetate or fMLF. Our results suggest that the lectin interferes with Toll-like receptor 2 and thus can impair the production of reactive oxygen species by host phagocytes. The work was supported by the Czech Science Foundation (grants no. 17-03253S and 18-18964S).

Návaznosti

• GA17-03253S, projekt VaV: Název: Hormonální kontrola hmyzího obranného systému

Investor: Grantová agentura ČR, Hormonální kontrola hmyzího obranného systému

• GA18-18964S, projekt VaV: Název: Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání

Investor: Grantová agentura ČR, Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání