J 2019

Biological effects of the benzotriazole ultraviolet stabilizers UV-234 and UV-320 in early-staged zebrafish (Danio rerio)

LIANG, Xuefang, Ondřej ADAMOVSKÝ, Christopher L. SOUDERS a Christopher J. MARTYNIUK

Základní údaje

Originální název

Biological effects of the benzotriazole ultraviolet stabilizers UV-234 and UV-320 in early-staged zebrafish (Danio rerio)

Autoři

LIANG, Xuefang (156 Čína), Ondřej ADAMOVSKÝ (203 Česká republika, garant, domácí), Christopher L. SOUDERS (840 Spojené státy) a Christopher J. MARTYNIUK (840 Spojené státy)

Vydání

Environmental Pollution, Oxford, ELSEVIER SCI LTD, 2019, 0269-7491

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 6.793

Kód RIV

RIV/00216224:14310/19:00110371

Organizační jednotka

Přírodovědecká fakulta

DOI

UT WoS

000457511900029

Klíčová slova anglicky

Benzotriazole ultraviolet stabilizers; Hatching delay; Mitochondrial bioenergetics; Oxidative stress; Immune response

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 24. 3. 2020 09:41, Mgr. Marie Šípková, DiS.

Anotace

V originále

Among the benzotriazole ultraviolet stabilizers (BUVSs), UV-234 and UV-320 are frequently detected in aquatic ecosystem. Despite the fact that these chemicals are present in low ng/L. levels in surface water, they show high bio-accumulation potential and pose exposure risks to aquatic organisms. However, there are limited toxicological data available in fish. In this study, zebrafish embryos were exposed to 0.01, at and 1 mu M UV-234 or UV-320 for up to 6 days. Developmental toxicity as well as effects on mitochondrial bioenergetics, immune system responses, and locomotor activity in zebrafish were measured. After UV-234 treatment (0.1-1 mu M), hatching time of embryos was increased compared to controls. There was also a similar to 20-40% reduction in non-mitochondrial respiration and oligomycin-dependent mitochondria! respiration in embryos treated with 1 mu M UV-234 for 24 and 48 h respectively; conversely basal respiration and non-mitochondrial respiration were increased similar to 20-30% in embryos treated with 1 mu M UV-320 at 48 h. Transcript levels of sod1 were down-regulated with BUVSs while sod2 mRNA was highly up-regulated with both UV-234 and UV-320, suggesting an oxidative damage response. Considering that mitochondrial signaling regulates innate immune pathways, we measured the expression of immune related transcripts (tlr5a, tlr5b, mmp9, il8, tnfa, cxcl-C1c, nJkb1, and ifng). Of these, only il8 and cxcl-C1c mRNA were decreased in response to 0.1 mu M UV-320, To associate early molecular events with behavior, locomotor activity was assessed. UV-234 reduced larval activity in a dark photokinesis assay by similar to 15%, however behavioral responses at environmentally-relevant concentrations of BUVSs were not consistent across experiments nor BUVSs. These data suggest that BUVSs can perturb mitochondria' bioenergetics, embryonic development, and locomotor activity of zebrafish, but these responses appear to be dose-, time- and BUVSs dependent, suggesting these chemicals may have unique modes of action.

Návaznosti

EF16_013/0001761, projekt VaV
Název: RECETOX RI
LM2015051, projekt VaV
Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX
707241, interní kód MU
Název: Environmental chemicals as obesogens (Akronym: OBESOGENS)
Investor: Evropská unie, Environmental chemicals as obesogens, MSCA Marie Skłodowska-Curie Actions (Excellent Science)