Possible methods for evaluation of hydrogen sulfide toxicity
against lactic acid bacteria

J 2019

Possible methods for evaluation of hydrogen sulfide toxicity against lactic acid bacteria

KOTRSOVÁ, Věra and Ivan KUSHKEVYCH

Basic information

Original name

Possible methods for evaluation of hydrogen sulfide toxicity against lactic acid bacteria

Authors

KOTRSOVÁ, Věra (203 Czech Republic, belonging to the institution) and Ivan KUSHKEVYCH (804 Ukraine, belonging to the institution)

Edition

Biointerface Research in Applied Chemistry, 2019, 2069-5837

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Romania

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

RIV identification code

RIV/00216224:14310/19:00110411

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.33263/BRIAC94.066069

UT WoS

000486974400013

Keywords in English

hydrogen sulfide; toxicity; lactic acid bacteria; sulfate-reducing bacteria

Abstract

V originále

An integral part of the intestinal microbiota is undoubtedly formed by lactic acid bacteria (LAB). Their presence in the digestive tract is essential for its proper functioning. During inflammatory bowel disease, such as ulcerative colitis (UC), LAB occurrence is reduced while sulfate-reducing bacteria (SRB) occur widely in the intestine resulting in an increase of their metabolite, hydrogen sulfide. Inhibitory concentration and mechanism of action of hydrogen sulfide on LAB are not fully known yet. The aim of this paper is to find the proper testing methods for evaluation of the interaction between hydrogen sulfide and lactic acid bacteria, including minimal inhibitory concentration (MIC) determination. Spectrophotometric, colorimetric, agar plate methods and combination of these methods were tested. Thousands of people from all over the world are affected by inflammatory bowel disease every year. The gained results could help to understand and improve the stability of intestinal microbiome, improve the treatment of bowel inflammation diseases, or prevent bowel disease altogether.

Links

MUNI/A/0902/2018, interní kód MU

Name: Podpora výzkumné činnosti studentů Mikrobiologie a molekulární biotechnologie 3

Investor: Masaryk University, Category A

Citovat

KOTRSOVÁ, Věra and Ivan KUSHKEVYCH. Possible methods for evaluation of hydrogen sulfide toxicity against lactic acid bacteria. Biointerface Research in Applied Chemistry. 2019, vol. 9, No 4, p. 4066-4069. ISSN 2069-5837. Available from: https://dx.doi.org/10.33263/BRIAC94.066069.

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   author = {Kotrsová, Věra and Kushkevych, Ivan},
   article_number = {4},
   doi = {http://dx.doi.org/10.33263/BRIAC94.066069},
   keywords = {hydrogen sulfide; toxicity; lactic acid bacteria; sulfate-reducing bacteria},
   language = {eng},
   issn = {2069-5837},
   journal = {Biointerface Research in Applied Chemistry},
   title = {Possible methods for evaluation of hydrogen sulfide toxicity against lactic acid bacteria},
   url = {http://biointerfaceresearch.com/?page_id=4269},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1549460
AU  - Kotrsová, Věra - Kushkevych, Ivan
PY  - 2019
TI  - Possible methods for evaluation of hydrogen sulfide toxicity against lactic acid bacteria
JF  - Biointerface Research in Applied Chemistry
VL  - 9
IS  - 4
SP  - 4066-4069
EP  - 4066-4069
SN  - 20695837
KW  - hydrogen sulfide
KW  - toxicity
KW  - lactic acid bacteria
KW  - sulfate-reducing bacteria
UR  - http://biointerfaceresearch.com/?page_id=4269
L2  - http://biointerfaceresearch.com/?page_id=4269
N2  - An integral part of the intestinal microbiota is undoubtedly formed by lactic acid bacteria (LAB). Their presence in the digestive tract is essential for its proper functioning. During inflammatory bowel disease, such as ulcerative colitis (UC), LAB occurrence is reduced while sulfate-reducing bacteria (SRB) occur widely in the intestine resulting in an increase of their metabolite, hydrogen sulfide. Inhibitory concentration and mechanism of action of hydrogen sulfide on LAB are not fully known yet. The aim of this paper is to find the proper testing methods for evaluation of the interaction between hydrogen sulfide and lactic acid bacteria, including minimal inhibitory concentration (MIC) determination. Spectrophotometric, colorimetric, agar plate methods and combination of these methods were tested. Thousands of people from all over the world are affected by inflammatory bowel disease every year. The gained results could help to understand and improve the stability of intestinal microbiome, improve the treatment of bowel inflammation diseases, or prevent bowel disease altogether.
ER  -

KOTRSOVÁ, Věra and Ivan KUSHKEVYCH. Possible methods for evaluation of hydrogen sulfide toxicity against lactic acid bacteria. \textit{Biointerface Research in Applied Chemistry}. 2019, vol.~9, No~4, p.~4066-4069. ISSN~2069-5837. Available from: https://dx.doi.org/10.33263/BRIAC94.066069.

Detailed Information on Publication Record