The Effect of Butyrate-Supplemented Parenteral Nutrition on
Intestinal Defence Mechanisms and the Parenteral
Nutrition-Induced Shift in the Gut Microbiota in the Rat Model

J 2019

The Effect of Butyrate-Supplemented Parenteral Nutrition on Intestinal Defence Mechanisms and the Parenteral Nutrition-Induced Shift in the Gut Microbiota in the Rat Model

JIRSOVA, Zuzana, Marie HECZKOVA, Helena DANKOVA, Hana MALINSKA, Petra VÍDEŇSKÁ et. al.

Základní údaje

Originální název

The Effect of Butyrate-Supplemented Parenteral Nutrition on Intestinal Defence Mechanisms and the Parenteral Nutrition-Induced Shift in the Gut Microbiota in the Rat Model

Autoři

JIRSOVA, Zuzana (203 Česká republika), Marie HECZKOVA (203 Česká republika), Helena DANKOVA (203 Česká republika), Hana MALINSKA, Petra VÍDEŇSKÁ (203 Česká republika, domácí), Hana VESPALCOVÁ (203 Česká republika, domácí), Lenka MICENKOVÁ (703 Slovensko, domácí), Lenka BARTONOVA (203 Česká republika), Eva STICOVA (203 Česká republika), Alena LODEREROVA (203 Česká republika), Lucia PREFERTUSOVA (203 Česká republika), Alena SEKERKOVA (203 Česká republika), Jaromir HRADECKY (203 Česká republika) a Monika CAHOVA (203 Česká republika)

Vydání

Biomed Research International, New York, Hindawi Publishing Corporation, 2019, 2314-6133

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20800 2.8 Environmental biotechnology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.276

Kód RIV

RIV/00216224:14310/19:00110419

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1155/2019/7084734

UT WoS

000460871500001

Klíčová slova anglicky

REGULATORY T-CELLS; DENDRITIC CELLS; IMMUNE; HOST; METABOLITES; EXPRESSION; DIFFERENTIATION; INFLAMMATION; MATURATION; MOLECULE

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2020 11:21, Mgr. Marie Šípková, DiS.

Anotace

V originále

Butyrate produced by the intestinal microbiota is essential for proper functioning of the intestinal immune system. Total dependence on parenteral nutrition (PN) is associated with numerous adverse effects, including severe microbial dysbiosis and loss of important butyrate producers. We hypothesised that a lack of butyrate produced by the gut microbiota may be compensated by its supplementation in PN mixtures. We tested whether i.v. butyrate administration would (a) positively modulate intestinal defence mechanisms and (b) counteract PN-induced dysbiosis. Male Wistar rats were randomised to chow, PN, and PN supplemented with 9 mM butyrate (PN+But) for 12 days. Antimicrobial peptides, mucins, tight junction proteins, and cytokine expression were assessed by RT-qPCR. T-cell subpopulations in mesenteric lymph nodes (MLN) were analysed by flow cytometry. Microbiota composition was assessed in caecum content. Butyrate supplementation resulted in increased expression of tight junction proteins (ZO-1, claudin-7, E-cadherin), antimicrobial peptides (Defa 8, Rd5, RegIII), and lysozyme in the ileal mucosa. Butyrate partially alleviated PN-induced intestinal barrier impairment and normalised IL-4, IL-10, and IgA mRNA expression. PN administration was associated with an increase in Tregs in MLN, which was normalised by butyrate. Butyrate increased the total number of CD4+ and decreased a relative amount of CD8+ memory T cells in MLN. Lack of enteral nutrition and PN administration led to a shift in caecal microbiota composition. Butyrate did not reverse the altered expression of most taxa but did influence the abundance of some potentially beneficial/pathogenic genera, which might contribute to its overall beneficial effect.

Citovat

JIRSOVA, Zuzana, Marie HECZKOVA, Helena DANKOVA, Hana MALINSKA, Petra VÍDEŇSKÁ, Hana VESPALCOVÁ, Lenka MICENKOVÁ, Lenka BARTONOVA, Eva STICOVA, Alena LODEREROVA, Lucia PREFERTUSOVA, Alena SEKERKOVA, Jaromir HRADECKY a Monika CAHOVA. The Effect of Butyrate-Supplemented Parenteral Nutrition on Intestinal Defence Mechanisms and the Parenteral Nutrition-Induced Shift in the Gut Microbiota in the Rat Model. Biomed Research International. New York: Hindawi Publishing Corporation, 2019, Neuveden, February, s. 1-14. ISSN 2314-6133. Dostupné z: https://dx.doi.org/10.1155/2019/7084734.

@article{1549505,
   author = {Jirsova, Zuzana and Heczkova, Marie and Dankova, Helena and Malinska, Hana and Vídeňská, Petra and Vespalcová, Hana and Micenková, Lenka and Bartonova, Lenka and Sticova, Eva and Lodererova, Alena and Prefertusova, Lucia and Sekerkova, Alena and Hradecky, Jaromir and Cahova, Monika},
   article_location = {New York},
   article_number = {February},
   doi = {http://dx.doi.org/10.1155/2019/7084734},
   keywords = {REGULATORY T-CELLS; DENDRITIC CELLS; IMMUNE; HOST; METABOLITES; EXPRESSION; DIFFERENTIATION; INFLAMMATION; MATURATION; MOLECULE},
   language = {eng},
   issn = {2314-6133},
   journal = {Biomed Research International},
   title = {The Effect of Butyrate-Supplemented Parenteral Nutrition on Intestinal Defence Mechanisms and the Parenteral Nutrition-Induced Shift in the Gut Microbiota in the Rat Model},
   url = {https://www.hindawi.com/journals/bmri/2019/7084734/},
   volume = {Neuveden},
   year = {2019}
}

TY  - JOUR
ID  - 1549505
AU  - Jirsova, Zuzana - Heczkova, Marie - Dankova, Helena - Malinska, Hana - Vídeňská, Petra - Vespalcová, Hana - Micenková, Lenka - Bartonova, Lenka - Sticova, Eva - Lodererova, Alena - Prefertusova, Lucia - Sekerkova, Alena - Hradecky, Jaromir - Cahova, Monika
PY  - 2019
TI  - The Effect of Butyrate-Supplemented Parenteral Nutrition on Intestinal Defence Mechanisms and the Parenteral Nutrition-Induced Shift in the Gut Microbiota in the Rat Model
JF  - Biomed Research International
VL  - Neuveden
IS  - February
SP  - 1-14
EP  - 1-14
PB  - Hindawi Publishing Corporation
SN  - 23146133
KW  - REGULATORY T-CELLS
KW  - DENDRITIC CELLS
KW  - IMMUNE
KW  - HOST
KW  - METABOLITES
KW  - EXPRESSION
KW  - DIFFERENTIATION
KW  - INFLAMMATION
KW  - MATURATION
KW  - MOLECULE
UR  - https://www.hindawi.com/journals/bmri/2019/7084734/
L2  - https://www.hindawi.com/journals/bmri/2019/7084734/
N2  - Butyrate produced by the intestinal microbiota is essential for proper functioning of the intestinal immune system. Total dependence on parenteral nutrition (PN) is associated with numerous adverse effects, including severe microbial dysbiosis and loss of important butyrate producers. We hypothesised that a lack of butyrate produced by the gut microbiota may be compensated by its supplementation in PN mixtures. We tested whether i.v. butyrate administration would (a) positively modulate intestinal defence mechanisms and (b) counteract PN-induced dysbiosis. Male Wistar rats were randomised to chow, PN, and PN supplemented with 9 mM butyrate (PN+But) for 12 days. Antimicrobial peptides, mucins, tight junction proteins, and cytokine expression were assessed by RT-qPCR. T-cell subpopulations in mesenteric lymph nodes (MLN) were analysed by flow cytometry. Microbiota composition was assessed in caecum content. Butyrate supplementation resulted in increased expression of tight junction proteins (ZO-1, claudin-7, E-cadherin), antimicrobial peptides (Defa 8, Rd5, RegIII), and lysozyme in the ileal mucosa. Butyrate partially alleviated PN-induced intestinal barrier impairment and normalised IL-4, IL-10, and IgA mRNA expression. PN administration was associated with an increase in Tregs in MLN, which was normalised by butyrate. Butyrate increased the total number of CD4+ and decreased a relative amount of CD8+ memory T cells in MLN. Lack of enteral nutrition and PN administration led to a shift in caecal microbiota composition. Butyrate did not reverse the altered expression of most taxa but did influence the abundance of some potentially beneficial/pathogenic genera, which might contribute to its overall beneficial effect.
ER  -

JIRSOVA, Zuzana, Marie HECZKOVA, Helena DANKOVA, Hana MALINSKA, Petra VÍDEŇSKÁ, Hana VESPALCOVÁ, Lenka MICENKOVÁ, Lenka BARTONOVA, Eva STICOVA, Alena LODEREROVA, Lucia PREFERTUSOVA, Alena SEKERKOVA, Jaromir HRADECKY a Monika CAHOVA. The Effect of Butyrate-Supplemented Parenteral Nutrition on Intestinal Defence Mechanisms and the Parenteral Nutrition-Induced Shift in the Gut Microbiota in the Rat Model. \textit{Biomed Research International}. New York: Hindawi Publishing Corporation, 2019, Neuveden, February, s.~1-14. ISSN~2314-6133. Dostupné z: https://dx.doi.org/10.1155/2019/7084734.

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