| MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ a Michaela WIMMEROVÁ. Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins. PLOS ONE. Public Library of Science, 2019, roč. 14, č. 7, s. 1-23. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0220318. |
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@article{1549857,
author = {Mrázková, Jana and Malinovská, Lenka and Wimmerová, Michaela},
article_number = {7},
doi = {http://dx.doi.org/10.1371/journal.pone.0220318},
keywords = {lectins; microscopy; agglutination; specificity},
language = {eng},
issn = {1932-6203},
journal = {PLOS ONE},
title = {Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins},
url = {https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable},
volume = {14},
year = {2019}
}
TY - JOUR
ID - 1549857
AU - Mrázková, Jana - Malinovská, Lenka - Wimmerová, Michaela
PY - 2019
TI - Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins
JF - PLOS ONE
VL - 14
IS - 7
SP - 1-23
EP - 1-23
PB - Public Library of Science
SN - 19326203
KW - lectins
KW - microscopy
KW - agglutination
KW - specificity
UR - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable
L2 - https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0220318&type=printable
N2 - Lectins are a group of ubiquitous proteins which specifically recognize and reversibly bind sugar moieties of glycoprotein and glycolipid constituents on cell surfaces. The mutagenesis approach is often employed to characterize lectin binding properties. As lectins are not enzymes, it is not easy to perform a rapid specificity screening of mutants using chromogenic substrates. Lectin-based cell agglutination is a simple and low-cost technique which can overcome most of these problems. In this work, a modified method of the agglutination of human erythrocytes and yeast cells with microscopy detection was successfully used for a specificity study of the newly prepared mutant lectin RS-IIL_A22S, which experimentally completed studies on sugar preferences of lectins in the PA-IIL family. Results showed that the sensitivity of this method is comparable with ITC, is able to determine subtle differences in lectin specificity, and works directly in cell lysates.
ER -
MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ a Michaela WIMMEROVÁ. Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins. \textit{PLOS ONE}. Public Library of Science, 2019, roč.~14, č.~7, s.~1-23. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0220318.
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