The detection of DNA methylation of tumour suppressor genes in
cervical high-grade squamous intraepithelial lesion: A
prospective cytological-histological correlation study of 70
cases

J 2019

The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases

ONDIČ, Ondrej, Jana NĚMCOVÁ, Reza ALAGHEHBANDAN, Kateřina ČERNÁ, Barbora GOMOLČÁKOVÁ et. al.

Základní údaje

Originální název

The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases

Autoři

ONDIČ, Ondrej (203 Česká republika, garant), Jana NĚMCOVÁ (203 Česká republika), Reza ALAGHEHBANDAN (124 Kanada), Kateřina ČERNÁ (203 Česká republika), Barbora GOMOLČÁKOVÁ (203 Česká republika), Iva KINKOROVÁ-LUŇÁČKOVÁ (203 Česká republika), Jan CHYTRA (203 Česká republika), Henrieta ŠIDLOVÁ (703 Slovensko), Ondřej MÁJEK (203 Česká republika, domácí) a Jiří BOUDA (203 Česká republika)

Vydání

CYTOPATHOLOGY, HOBOKEN, WILEY, 2019, 0956-5507

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.493

Kód RIV

RIV/00216224:14110/19:00110577

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1111/cyt.12718

UT WoS

000472237900011

Klíčová slova anglicky

cervix; cytology; HSIL; hypermethylation; methylation

Štítky

14119612, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 9. 2019 15:18, Mgr. Tereza Miškechová

Anotace

V originále

Background DNA methylation has been suggested as one of the epigenetic changes promoting carcinogenesis. The aim of this study was to prospectively evaluate the methylation status of CADM 1, MAL and hsa-miR-124 genes in high-grade squamous intraepithelial lesion (HSIL) liquid-based cytology (LBC) samples with a histological correlation. Methods Seventy histologically confirmed cases of HSIL paired with prior screening LBC diagnosis of HSIL within a 3-month interval were selected. Histologically, the lesions were reviewed and assessed including: (a) number of blocks harbouring dysplastic squamous epithelium; (b) number of blocks containing glandular extension of dysplastic epithelium; and (c) the depth of glandular extension (which was assessed semi-quantitatively as graded 1-3). Human papillomavirus (HPV) subtyping was performed from residual LBC materials using the LINEAR ARRAY HPV Genotyping Test and in-house polymerase chain reaction targeting the HPV E1 gene. The detection of methylation silencing of tumour suppressor genes CADM1, MAL and hsa-miR-124 was performed by multiplex methylation-specific real-time polymerase chain reaction. Results A positive methylation status was detected in 41 cases (58.6%). The number of blocks with HSIL varied from one to 13. Glandular extension was seen in 44 cases with the number of blocks involved ranging from one to 10. The depth of HSIL glandular extension varied. Conclusion The DNA methylation test allows HSIL lesions to be divided into two distinct groups of methylated HSIL in significantly older patients and unmethylated HSIL in younger patients. This study was not able to prove that methylation status in cervical HSIL correlates with the size of the lesion (measured by the number of blocks involved) or with HSIL propensity for endocervical glandular extension, nor with HPV type or multi-infection.

Citovat

ONDIČ, Ondrej, Jana NĚMCOVÁ, Reza ALAGHEHBANDAN, Kateřina ČERNÁ, Barbora GOMOLČÁKOVÁ, Iva KINKOROVÁ-LUŇÁČKOVÁ, Jan CHYTRA, Henrieta ŠIDLOVÁ, Ondřej MÁJEK a Jiří BOUDA. The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases. CYTOPATHOLOGY. HOBOKEN: WILEY, 2019, roč. 30, č. 4, s. 426-431. ISSN 0956-5507. Dostupné z: https://dx.doi.org/10.1111/cyt.12718.

@article{1552438,
   author = {Ondič, Ondrej and Němcová, Jana and Alaghehbandan, Reza and Černá, Kateřina and Gomolčáková, Barbora and KinkorováandLuňáčková, Iva and Chytra, Jan and Šidlová, Henrieta and Májek, Ondřej and Bouda, Jiří},
   article_location = {HOBOKEN},
   article_number = {4},
   doi = {http://dx.doi.org/10.1111/cyt.12718},
   keywords = {cervix; cytology; HSIL; hypermethylation; methylation},
   language = {eng},
   issn = {0956-5507},
   journal = {CYTOPATHOLOGY},
   title = {The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases},
   url = {http://dx.doi.org/10.1111/cyt.12718},
   volume = {30},
   year = {2019}
}

TY  - JOUR
ID  - 1552438
AU  - Ondič, Ondrej - Němcová, Jana - Alaghehbandan, Reza - Černá, Kateřina - Gomolčáková, Barbora - Kinkorová-Luňáčková, Iva - Chytra, Jan - Šidlová, Henrieta - Májek, Ondřej - Bouda, Jiří
PY  - 2019
TI  - The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases
JF  - CYTOPATHOLOGY
VL  - 30
IS  - 4
SP  - 426-431
EP  - 426-431
PB  - WILEY
SN  - 09565507
KW  - cervix
KW  - cytology
KW  - HSIL
KW  - hypermethylation
KW  - methylation
UR  - http://dx.doi.org/10.1111/cyt.12718
L2  - http://dx.doi.org/10.1111/cyt.12718
N2  - Background DNA methylation has been suggested as one of the epigenetic changes promoting carcinogenesis. The aim of this study was to prospectively evaluate the methylation status of CADM 1, MAL and hsa-miR-124 genes in high-grade squamous intraepithelial lesion (HSIL) liquid-based cytology (LBC) samples with a histological correlation. Methods Seventy histologically confirmed cases of HSIL paired with prior screening LBC diagnosis of HSIL within a 3-month interval were selected. Histologically, the lesions were reviewed and assessed including: (a) number of blocks harbouring dysplastic squamous epithelium; (b) number of blocks containing glandular extension of dysplastic epithelium; and (c) the depth of glandular extension (which was assessed semi-quantitatively as graded 1-3). Human papillomavirus (HPV) subtyping was performed from residual LBC materials using the LINEAR ARRAY HPV Genotyping Test and in-house polymerase chain reaction targeting the HPV E1 gene. The detection of methylation silencing of tumour suppressor genes CADM1, MAL and hsa-miR-124 was performed by multiplex methylation-specific real-time polymerase chain reaction. Results A positive methylation status was detected in 41 cases (58.6%). The number of blocks with HSIL varied from one to 13. Glandular extension was seen in 44 cases with the number of blocks involved ranging from one to 10. The depth of HSIL glandular extension varied. Conclusion The DNA methylation test allows HSIL lesions to be divided into two distinct groups of methylated HSIL in significantly older patients and unmethylated HSIL in younger patients. This study was not able to prove that methylation status in cervical HSIL correlates with the size of the lesion (measured by the number of blocks involved) or with HSIL propensity for endocervical glandular extension, nor with HPV type or multi-infection.
ER  -

ONDIČ, Ondrej, Jana NĚMCOVÁ, Reza ALAGHEHBANDAN, Kateřina ČERNÁ, Barbora GOMOLČÁKOVÁ, Iva KINKOROVÁ-LUŇÁČKOVÁ, Jan CHYTRA, Henrieta ŠIDLOVÁ, Ondřej MÁJEK a Jiří BOUDA. The detection of DNA methylation of tumour suppressor genes in cervical high-grade squamous intraepithelial lesion: A prospective cytological-histological correlation study of 70 cases. \textit{CYTOPATHOLOGY}. HOBOKEN: WILEY, 2019, roč.~30, č.~4, s.~426-431. ISSN~0956-5507. Dostupné z: https://dx.doi.org/10.1111/cyt.12718.

Podrobný výpis o publikaci