a 2019

PORCN inhibition stimulates chondrocyte differentiation

KILLINGER, Michael a marcela BUCHTOVÁ

Základní údaje

Originální název

PORCN inhibition stimulates chondrocyte differentiation

Autoři

KILLINGER, Michael a marcela BUCHTOVÁ

Vydání

International Congress of Vertebrate Morphology. 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30106 Anatomy and morphology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 1.563

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00110591

Organizační jednotka

Přírodovědecká fakulta

ISSN

UT WoS

Klíčová slova anglicky

morphology

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 21. 4. 2020 17:05, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Porcupine (PORCN) is an endoplasmic reticulum protein belonging tothe membrane bound O–acyl transferase superfamily. This molecule isnecessary for the attachment of long chain fatty acids to WNT pro-teins. PORCN is expressed in most tissues of the body and animalswith loss of function in PORCN exhibit embryonic lethality at earlydevelopmental stages with extensive gastrulation defects. Severaldominant mutations in PORCN were described in human patients,who demonstrated significant skeletal abnormalities. Recently, a pow-erful PORCN inhibitor C59 has become commercially available andhas been successfully used to inhibit WNT pathway in several cancerlines. C59 negatively affects WNT signaling by inhibition of WNTpalmitoylation, which is necessary to acetylate WNT ligands beforetheir secretion and binding to a carrier protein. Here, we analyzed thepossible effect of this inhibitor on skeletogenesis with focus on endo-chondral bone formation. We observed, enhanced growth of mesen-chymal condensations and also increased number of cartilage nodulesin primary micromass cultures established from the early limb bud,which were treated by increasing concentrations of C59.