Laser-Induced Breakdown Spectroscopy as a Novel Readout Method
for Nanoparticle-Based Immunoassays

a 2019

Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays

MODLITBOVÁ, Pavlína; Zdeněk FARKA; Matěj PASTUCHA; Pavel POŘÍZKA; Karel NOVOTNÝ et al.

Základní údaje

Originální název

Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays

Autoři

MODLITBOVÁ, Pavlína; Zdeněk FARKA; Matěj PASTUCHA; Pavel POŘÍZKA; Karel NOVOTNÝ; Petr SKLÁDAL a Jozef KAISER

Vydání

EMSLIBS 2019: 10th Euro-Mediterranean Symposium on Laser-Induced Breakdown Spectroscopy, 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

ISBN

978-80-88195-13-9

Příznaky

Mezinárodní význam

Změněno: 7. 9. 2019 12:53, doc. Mgr. Zdeněk Farka, Ph.D.

Anotace

V originále

Laser-Induced Breakdown Spectroscopy (LIBS) was examined as a novel method to provide the readout of microtiter plate immunoassays involving nanoparticles (NP). The so-called Tag-LIBS, i.e. a sensitive detection of specific biomarkers, was applied to detect the NP labels using the nanosecond ablation sampling. The NP labels were examined from the bottom of the standard 96-well microtiter plate. Thanks to the well-known flexibility in the LIBS instrumentation, the plasma emission collection as well as the focusing optics can be rebuilt into a collinear arrangement. The presented experiments proved the suitable detection of silver NPs from the microtiter plate’s bottom without any negative effects on the NPs’ qualitative and quantitative analysis. Subsequently, a sandwich immunoassay tha tdetects human serum albumin was successfully developed, using streptavidin-coated AgNP labels. The assay provided a detection limit of 10 ng/mL, which is comparable with the fluorescence-based readout. The main advantage of LIBS readout is that it enables a detection of any type of NP labels independent on their fluorescence or catalytic properties. Owing to the immediate signal response, a relatively simple instrumentation enabling the automation, and the capability of multi-elemental analyses, LIBS technique offers a promising and fast alternative to other readout techniques allowing a multiplex detection of various biomarkers.

Návaznosti

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MODLITBOVÁ, Pavlína; Zdeněk FARKA; Matěj PASTUCHA; Pavel POŘÍZKA; Karel NOVOTNÝ; Petr SKLÁDAL a Jozef KAISER. Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays. In EMSLIBS 2019: 10th Euro-Mediterranean Symposium on Laser-Induced Breakdown Spectroscopy. 2019. ISBN 978-80-88195-13-9.

@proceedings{1554077,
   author = {Modlitbová, Pavlína and Farka, Zdeněk and Pastucha, Matěj and Pořízka, Pavel and Novotný, Karel and Skládal, Petr and Kaiser, Jozef},
   booktitle = {EMSLIBS 2019: 10th Euro-Mediterranean Symposium on Laser-Induced Breakdown Spectroscopy},
   language = {eng},
   isbn = {978-80-88195-13-9},
   title = {Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays},
   url = {https://emslibs.com/wp-content/uploads/2019/09/b3.2.pdf},
   year = {2019}
}

TY  - CONF
ID  - 1554077
AU  - Modlitbová, Pavlína - Farka, Zdeněk - Pastucha, Matěj - Pořízka, Pavel - Novotný, Karel - Skládal, Petr - Kaiser, Jozef
PY  - 2019
TI  - Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays
SN  - 9788088195139
UR  - https://emslibs.com/wp-content/uploads/2019/09/b3.2.pdf
N2  - Laser-Induced Breakdown Spectroscopy (LIBS) was examined as a novel method to provide the readout of microtiter plate immunoassays involving nanoparticles (NP). The so-called Tag-LIBS, i.e. a sensitive detection of specific biomarkers, was applied to detect the NP labels using the nanosecond ablation sampling. The NP labels were examined from the bottom of the standard 96-well microtiter plate. Thanks to the well-known flexibility in the LIBS instrumentation, the plasma emission collection as well as the focusing optics can be rebuilt into a collinear arrangement. The presented experiments proved the suitable detection of silver NPs from the microtiter plate’s bottom without any negative effects on the NPs’ qualitative and quantitative analysis. Subsequently, a sandwich immunoassay tha tdetects human serum albumin was successfully developed, using streptavidin-coated AgNP labels. The assay provided a detection limit of 10 ng/mL, which is comparable with the fluorescence-based readout. The main advantage of LIBS readout is that it enables a detection of any type of NP labels independent on their fluorescence or catalytic properties. Owing to the immediate signal response, a relatively simple instrumentation enabling the automation, and the capability of multi-elemental analyses, LIBS technique offers a promising and fast alternative to other readout techniques allowing a multiplex detection of various biomarkers.
ER  -

MODLITBOVÁ, Pavlína; Zdeněk FARKA; Matěj PASTUCHA; Pavel POŘÍZKA; Karel NOVOTNÝ; Petr SKLÁDAL a Jozef KAISER. Laser-Induced Breakdown Spectroscopy as a Novel Readout Method for Nanoparticle-Based Immunoassays. In \textit{EMSLIBS 2019: 10th Euro-Mediterranean Symposium on Laser-Induced Breakdown Spectroscopy}. 2019. ISBN~978-80-88195-13-9.

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