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@proceedings{1559256,
author = {Svobodníková, Lucie and Kummerová, Marie and Zezulka, Štěpán and Babula, Petr},
booktitle = {Plant Biology CS 2019 (Bulletin CSEBR 1/2019 - Book of Abstracts)},
keywords = {tobacco; cell suspension; diclofenac; oxidative stress; apoptotic changes;},
language = {eng},
title = {Tobacco BY-2 cell suspension as a model for assessment of pharmaceuticals phytotoxicity},
year = {2019}
}
TY - CONF
ID - 1559256
AU - Svobodníková, Lucie - Kummerová, Marie - Zezulka, Štěpán - Babula, Petr
PY - 2019
TI - Tobacco BY-2 cell suspension as a model for assessment of pharmaceuticals phytotoxicity
KW - tobacco
KW - cell suspension
KW - diclofenac
KW - oxidative stress
KW - apoptotic changes;
N2 - Pharmaceuticals and pharmaceutically active substances nowadays represent an important class of emerging environmental pollutants. Growth and developmental changes in plants induced by pharmaceuticals reflect changes of processes at the cellular and subcellular levels. Due to their growth and cellular characteristics, plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity. Non-steroidal anti-inflammatory drug diclofenac (DCF) belongs to currently monitored compounds in the environment because of its frequent occurrence and high potential toxicity. This study suggests that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, suspension density and mitotic index. The growth and viability changes after 120 h of treatment were accompanied by increased production of total reactive oxygen species (ROS) and mitochondrial superoxide, and by down-regulation of reactive nitrogen species and total thiols in correlation to DCF treatment. DCF-induced oxidative stress triggered apoptotic alterations in BY-2 cells leading to programmed cell death – irregular or stretched nuclei were observed in up to 50% of cells. The response of the tobacco BY-2 cell suspension to DCF treatment is prompt and sensitive and selected parameters are suitable for toxicity evaluation.
ER -
SVOBODNÍKOVÁ, Lucie, Marie KUMMEROVÁ, Štěpán ZEZULKA a Petr BABULA. Tobacco BY-2 cell suspension as a model for assessment of pharmaceuticals phytotoxicity. In \textit{Plant Biology CS 2019 (Bulletin CSEBR 1/2019 - Book of Abstracts)}. 2019. ISSN~1213-6670.
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