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@proceedings{1559276,
author = {Svobodníková, Lucie and Kummerová, Marie and Zezulka, Štěpán and Babula, Petr},
booktitle = {Plant Biology CS 2019 (Bulletin CSEBR 1/2019 - Book of Abstracts)},
keywords = {Nicotiana tabacum; cell suspension BY-2; pharmaceuticals; mitotic index; oxidative stress; apoptosis;},
language = {eng},
title = {Biochemical and cytological changes of tobacco BY-2 cells under diclofenac impact},
year = {2019}
}
TY - CONF
ID - 1559276
AU - Svobodníková, Lucie - Kummerová, Marie - Zezulka, Štěpán - Babula, Petr
PY - 2019
TI - Biochemical and cytological changes of tobacco BY-2 cells under diclofenac impact
KW - Nicotiana tabacum
KW - cell suspension BY-2
KW - pharmaceuticals
KW - mitotic index
KW - oxidative stress
KW - apoptosis;
N2 - Studies focusing on environmental contamination have shown that besides “classic” persistent pollutants, pseudo-persistent emerging contaminants are also present. As highly specific biologically active substances, pharmaceuticals and pharmaceutically active substances entering the environment can influence non-target organisms including plants. One of the commonly detected and monitored pharmaceuticals in water is a non-steroidal anti-inflammatory drug diclofenac (DCF). Plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity of wide range of inorganic and organic pollutants at the cellular and subcellular levels. The question is, if suspension BY-2 can be used even for drug toxicity evaluation. Results of this study suggest that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, mitotic index, suspension density and dry weight. DCF-induced oxidative stress documented by increased production of total reactive oxygen species (ROS) triggered apoptotic alterations in BY-2 cells leading to programmed cell death – occurrence of acidic compartments and autophagic vacuoles in BY-2 cells and apoptotic chromatin condensation and DNA fragmentation in their nuclei. Due to its prompt and sensitive response tobacco BY-2 cell suspension is suitable for evaluation of phytotoxicity of drugs.
ER -
SVOBODNÍKOVÁ, Lucie, Marie KUMMEROVÁ, Štěpán ZEZULKA a Petr BABULA. Biochemical and cytological changes of tobacco BY-2 cells under diclofenac impact. In \textit{Plant Biology CS 2019 (Bulletin CSEBR 1/2019 - Book of Abstracts)}. 2019. ISSN~1213-6670.
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