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@article{1559636,
author = {Vargova, V and Helma, R and Palecek, E and Ostatna, V},
article_location = {Amsterdam},
doi = {http://dx.doi.org/10.1016/j.aca.2016.06.055},
keywords = {Protein-protein interactions; Lectin-glycoprotein interactions; Ovalbumin; Concanavalin A; Constant current chronopotentiometric stripping; Mercury electrodes},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Electrochemical sensing of concanavalin A and ovalbumin interaction in solution},
url = {http://dx.doi.org/10.1016/j.aca.2016.06.055},
volume = {935},
year = {2016}
}
TY - JOUR
ID - 1559636
AU - Vargova, V - Helma, R - Palecek, E - Ostatna, V
PY - 2016
TI - Electrochemical sensing of concanavalin A and ovalbumin interaction in solution
JF - Analytica Chimica Acta
VL - 935
SP - 97-103
EP - 97-103
PB - Elsevier Science publishers
SN - 00032670
KW - Protein-protein interactions
KW - Lectin-glycoprotein interactions
KW - Ovalbumin
KW - Concanavalin A
KW - Constant current chronopotentiometric stripping
KW - Mercury electrodes
UR - http://dx.doi.org/10.1016/j.aca.2016.06.055
L2 - http://dx.doi.org/10.1016/j.aca.2016.06.055
N2 - In an attempt to develop a label- and reagent-free electrochemical method for the detection of lectin-glycoprotein interactions, we tested lectin-concanavalin A (ConA), glycoprotein-ovalbumin (Ova) and their complex using chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode. Incubation of ConA with Ova resulted in an increase of the CPS peak H of the complex as compared to the CPS peaks of individual Ova and ConA proteins. Qualitatively similar results were obtained with other glycoprotein-lectin couples (ConA-RNase B and lectin from Sambucus nigra-fetuin). Using the CPS method, we were able to follow the course of complex formation in solution. Comparable responses of Ova, ConA and ConA-Ova complex were obtained not only at the mercury electrode but also with solid amalgam electrodes, which are more suitable for parallel analysis. It can be anticipated that electrochemical methods, namely CPS, will find application in glycomics and proteomics. (C) 2016 Elsevier B.V. All rights reserved.
ER -
VARGOVA, V, R HELMA, E PALECEK a V OSTATNA. Electrochemical sensing of concanavalin A and ovalbumin interaction in solution. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2016, roč.~935, s.~97-103. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2016.06.055.
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