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@article{1559636, author = {Vargova, V and Helma, R and Palecek, E and Ostatna, V}, article_location = {Amsterdam}, doi = {http://dx.doi.org/10.1016/j.aca.2016.06.055}, keywords = {Protein-protein interactions; Lectin-glycoprotein interactions; Ovalbumin; Concanavalin A; Constant current chronopotentiometric stripping; Mercury electrodes}, language = {eng}, issn = {0003-2670}, journal = {Analytica Chimica Acta}, title = {Electrochemical sensing of concanavalin A and ovalbumin interaction in solution}, url = {http://dx.doi.org/10.1016/j.aca.2016.06.055}, volume = {935}, year = {2016} }
TY - JOUR ID - 1559636 AU - Vargova, V - Helma, R - Palecek, E - Ostatna, V PY - 2016 TI - Electrochemical sensing of concanavalin A and ovalbumin interaction in solution JF - Analytica Chimica Acta VL - 935 SP - 97-103 EP - 97-103 PB - Elsevier Science publishers SN - 00032670 KW - Protein-protein interactions KW - Lectin-glycoprotein interactions KW - Ovalbumin KW - Concanavalin A KW - Constant current chronopotentiometric stripping KW - Mercury electrodes UR - http://dx.doi.org/10.1016/j.aca.2016.06.055 L2 - http://dx.doi.org/10.1016/j.aca.2016.06.055 N2 - In an attempt to develop a label- and reagent-free electrochemical method for the detection of lectin-glycoprotein interactions, we tested lectin-concanavalin A (ConA), glycoprotein-ovalbumin (Ova) and their complex using chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode. Incubation of ConA with Ova resulted in an increase of the CPS peak H of the complex as compared to the CPS peaks of individual Ova and ConA proteins. Qualitatively similar results were obtained with other glycoprotein-lectin couples (ConA-RNase B and lectin from Sambucus nigra-fetuin). Using the CPS method, we were able to follow the course of complex formation in solution. Comparable responses of Ova, ConA and ConA-Ova complex were obtained not only at the mercury electrode but also with solid amalgam electrodes, which are more suitable for parallel analysis. It can be anticipated that electrochemical methods, namely CPS, will find application in glycomics and proteomics. (C) 2016 Elsevier B.V. All rights reserved. ER -
VARGOVA, V, R HELMA, E PALECEK and V OSTATNA. Electrochemical sensing of concanavalin A and ovalbumin interaction in solution. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2016, vol.~935, p.~97-103. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2016.06.055.
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