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@article{1561176, author = {Schořová, Šárka and Fajkus, Jiří and Záveská Drábková, Lenka and Honys, David and Procházková Schrumpfová, Petra}, article_number = {2}, doi = {http://dx.doi.org/10.1111/tpj.14306}, keywords = {Telomerase assembly; Pontin; Reptin; AtTERT; AtTRB; AtRuvBL; AtPOT1a; nucleolus; Arabidopsis.}, language = {eng}, issn = {0960-7412}, journal = {The Plant Journal}, title = {The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis}, url = {https://onlinelibrary.wiley.com/doi/10.1111/tpj.14306}, volume = {98}, year = {2019} }
TY - JOUR ID - 1561176 AU - Schořová, Šárka - Fajkus, Jiří - Záveská Drábková, Lenka - Honys, David - Procházková Schrumpfová, Petra PY - 2019 TI - The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis JF - The Plant Journal VL - 98 IS - 2 SP - 195-212 EP - 195-212 PB - Blackwell Science SN - 09607412 KW - Telomerase assembly KW - Pontin KW - Reptin KW - AtTERT KW - AtTRB KW - AtRuvBL KW - AtPOT1a KW - nucleolus KW - Arabidopsis. UR - https://onlinelibrary.wiley.com/doi/10.1111/tpj.14306 L2 - https://onlinelibrary.wiley.com/doi/10.1111/tpj.14306 N2 - Telomerase maturation and recruitment to telomeres is regulated by several telomerase- and telomereassociated proteins. Among a number of proteins, human Pontin and Reptin play critical roles in telomerase biogenesis. Here we characterized plant orthologues of Pontin and Reptin, RuvBL1 and RuvBL2a, respectively, and show association of Arabidopsis thaliana RuvBL1 (AtRuvBL1) with the catalytic subunit of telomerase (AtTERT) in the nucleolus in vivo. In contrast to mammals, interactions between AtTERT and AtRuvBL proteins in A. thaliana are not direct and they are rather mediated by one of the Arabidopsis thaliana Telomere Repeat Binding (AtTRB) proteins. We further show that plant orthologue of dyskerin, named AtCBF5, is indirectly associated with AtTRB proteins but not with the AtRuvBL proteins in the plant nucleus/nucleolus, and interacts with the Protection of telomere 1 (AtPOT1a) in the nucleolus or cytoplasmic foci. Our genomewide phylogenetic analyses identify orthologues in RuvBL protein family within the plant kingdom. Dysfunction of AtRuvBL genes in heterozygous T-DNA insertion A. thaliana mutants results in reduced telomerase activity and indicate the involvement of AtRuvBL in plant telomerase biogenesis. ER -
SCHOŘOVÁ, Šárka, Jiří FAJKUS, Lenka ZÁVESKÁ DRÁBKOVÁ, David HONYS a Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis. \textit{The Plant Journal}. Blackwell Science, 2019, roč.~98, č.~2, s.~195-212. ISSN~0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.14306.
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