| FAJKUS, Petr, Vratislav PEŠKA, Michal ZÁVODNÍK, Miloslava FOJTOVÁ, Jana FULNEČKOVÁ, Šimon DOBIAS, Agata Magdalena KILAR, Martina DVOŘÁČKOVÁ, Dagmar ZACHOVÁ, Ivona NEČASOVÁ, Jason SIMS, Eva SÝKOROVÁ a Jiří FAJKUS. Telomerase RNAs in land plants. Nucleic acids research. Oxford: Oxford University Press, 2019, roč. 47, č. 18, s. 9842-9856. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkz695. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1561456,
author = {Fajkus, Petr and Peška, Vratislav and Závodník, Michal and Fojtová, Miloslava and Fulnečková, Jana and Dobias, Šimon and Kilar, Agata Magdalena and Dvořáčková, Martina and Zachová, Dagmar and Nečasová, Ivona and Sims, Jason and Sýkorová, Eva and Fajkus, Jiří},
article_location = {Oxford},
article_number = {18},
doi = {http://dx.doi.org/10.1093/nar/gkz695},
keywords = {Telomerase RNA subunit; plant},
language = {eng},
issn = {0305-1048},
journal = {Nucleic acids research},
title = {Telomerase RNAs in land plants},
url = {https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz695/5545009},
volume = {47},
year = {2019}
}
TY - JOUR
ID - 1561456
AU - Fajkus, Petr - Peška, Vratislav - Závodník, Michal - Fojtová, Miloslava - Fulnečková, Jana - Dobias, Šimon - Kilar, Agata Magdalena - Dvořáčková, Martina - Zachová, Dagmar - Nečasová, Ivona - Sims, Jason - Sýkorová, Eva - Fajkus, Jiří
PY - 2019
TI - Telomerase RNAs in land plants
JF - Nucleic acids research
VL - 47
IS - 18
SP - 9842-9856
EP - 9842-9856
PB - Oxford University Press
SN - 03051048
KW - Telomerase RNA subunit
KW - plant
UR - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz695/5545009
L2 - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkz695/5545009
N2 - To elucidate the molecular nature of evolutionary changes of telomeres in the plant order Asparagales, we aimed to characterize telomerase RNA subunits (TRs) in these plants. The unusually long telomere repeat unit in Allium plants (12 nt) allowed us to identify TRs in transcriptomic data of representative species of the Allium genus. Orthologous TRs were then identified in Asparagales plants harbouring telomere DNA composed of TTAGGG (human type) or TTTAGGG (Arabidopsis-type) repeats. Further, we identified TRs across the land plant phylogeny, including common model plants, crop plants, and plants with unusual telomeres. Several lines of functional testing demonstrate the templating telomerase function of the identified TRs and disprove a functionality of the only previously reported plant telomerase RNA in Arabidopsis thaliana. Importantly, our results change the existing paradigm in plant telomere biology which has been based on the existence of a relatively conserved telomerase reverse transcriptase subunit (TERT) associating with highly divergent TRs even between closely related plant taxa. The finding of a monophyletic origin of genuine TRs across land plants opens the possibility to identify TRs directly in transcriptomic or genomic data and/or predict telomere sequences synthesized according to the respective TR template region.
ER -
FAJKUS, Petr, Vratislav PEŠKA, Michal ZÁVODNÍK, Miloslava FOJTOVÁ, Jana FULNEČKOVÁ, Šimon DOBIAS, Agata Magdalena KILAR, Martina DVOŘÁČKOVÁ, Dagmar ZACHOVÁ, Ivona NEČASOVÁ, Jason SIMS, Eva SÝKOROVÁ a Jiří FAJKUS. Telomerase RNAs in land plants. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2019, roč.~47, č.~18, s.~9842-9856. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkz695.
|
