2019
Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids
MICHÁLEK, Jan, Karel ŠTĚPKA, Michal KOZUBEK, Jarmila NAVRÁTILOVÁ, Barbora PAVLATOVSKÁ et. al.Základní údaje
Originální název
Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids
Autoři
MICHÁLEK, Jan (203 Česká republika, garant, domácí), Karel ŠTĚPKA (203 Česká republika, domácí), Michal KOZUBEK (203 Česká republika, domácí), Jarmila NAVRÁTILOVÁ (203 Česká republika, domácí), Barbora PAVLATOVSKÁ (203 Česká republika, domácí), Markéta MACHÁLKOVÁ (203 Česká republika, domácí), Jan PREISLER (203 Česká republika, domácí) a Adam PRUŠKA (203 Česká republika, domácí)
Vydání
Microscopy and Microanalysis, Cambridge University Press, 2019, 1431-9276
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
20602 Medical laboratory technology ;
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 3.414
Kód RIV
RIV/00216224:14330/19:00110913
Organizační jednotka
Fakulta informatiky
UT WoS
000502313700003
Klíčová slova anglicky
confocal microscopy; image registration; MALDI MS; mass spectrometry imaging; peeling
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 15. 1. 2021 08:47, Mgr. Marie Šípková, DiS.
Anotace
V originále
Spheroids—three-dimensional aggregates of cells grown from a cancer cell line—represent a model of living tissue for chemotherapy inves- tigation. Distribution of chemotherapeutics in spheroid sections was determined using the matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Proliferating or apoptotic cells were immunohistochemically labeled and visualized by laser scanning confocal fluorescence microscopy (LSCM). Drug efficacy was evaluated by comparing coregistered MALDI MSI and LSCM data of drug- treated spheroids with LSCM only data of untreated control spheroids. We developed a fiducial-based workflow for coregistration of low- resolution MALDI MS with high-resolution LSCM images. To allow comparison of drug and cell distribution between the drug-treated and untreated spheroids of different shapes or diameters, we introduced a common diffusion-related coordinate, the distance from the spheroid boundary. In a procedure referred to as “peeling”, we correlated average drug distribution at a certain distance with the average reduction in the affected cells between the untreated and the treated spheroids. This novel approach makes it possible to differentiate between peripheral cells that died due to therapy and the innermost cells which died naturally. Two novel algorithms—for MALDI MS image denoising and for weighting of MALDI MSI and LSCM data by the presence of cell nuclei—are also presented.
Návaznosti
| EF16_013/0001775, projekt VaV |
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| LM2015062, projekt VaV |
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| LQ1601, projekt VaV |
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| LTC17016, projekt VaV |
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| MUNI/A/1359/2018, interní kód MU |
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| MUNI/G/0974/2016, interní kód MU |
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