Selectively Oxidized Cellulose with Adjustable Molecular Weight
for Controlled Release of Platinum Anticancer Drugs

J 2019

Selectively Oxidized Cellulose with Adjustable Molecular Weight for Controlled Release of Platinum Anticancer Drugs

MUNSTER, Lukas; Michaela FOJTŮ; Zdenka CAPAKOVA; Tomáš VACULOVIČ; Michaela TVRDOŇOVÁ et. al.

Základní údaje

Originální název

Selectively Oxidized Cellulose with Adjustable Molecular Weight for Controlled Release of Platinum Anticancer Drugs

Autoři

MUNSTER, Lukas; Michaela FOJTŮ; Zdenka CAPAKOVA; Tomáš VACULOVIČ; Michaela TVRDOŇOVÁ; Ivo KURITKA; Michal MASAŘÍK a Jan VICHA

Vydání

Biomacromolecules, Washington, American Chemical Society, 2019, 1525-7797

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.092

Kód RIV

RIV/00216224:14110/19:00107804

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1021/acs.biomac.8b01807

UT WoS

000464248300015

EID Scopus

2-s2.0-85063044087

Klíčová slova anglicky

NMR CHEMICAL-SHIFTS; DENSITY-FUNCTIONAL THEORY; RELATIVISTIC DOUBLE-ZETA; HYALURONIC-ACID; DIALDEHYDE CELLULOSE; TRIPLE-ZETA; BASIS-SETS; PT-195 NMR; CISPLATIN; PERIODATE

Štítky

14110515, 14110518, CF NMR, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 7. 2025 11:15, Mgr. Petra Trembecká, Ph.D.

Anotace

V originále

The synthesis of selectively oxidized cellulose, 2,3-dicarboxycellulose (DCC), is optimized for preparation of highly oxidized material for biological applications, which includes control over the molecular weight of the product during its synthesis. Conjugates of DCC and cisplatin simultaneously offer a very high drug binding efficiency (>90%) and drug loading capacity (up to 50 wt %), while retaining good aqueous solubility. The adjustable molecular weight of the DCC together with variances in drug feeding ratio allows to optimize cisplatin release profiles from delayed (<2% of cisplatin released during 6 h) to classical burst release with more than 60% of cisplatin released after 24 h. The release rates are also pH-dependent (up to 2 times faster release at pH 5.5 than at pH 7.4), which allows to exploit the acidic nature of tumor microenvironment. Extensive in vitro studies were performed on eight different cell lines for two cisplatin-DCC conjugates with different release profiles. In comparison with free cisplatin, both cisplatin-DCC conjugates demonstrated considerably lower cytotoxicity toward healthy cells. Conjugates with burst release profiles were found more effective against prostate cell lines, while DCC conjugates with slower release were more cytotoxic against ovarian and lung carcinoma cell lines. In vivo studies indicated a significantly longer survival rate, a reduction in tumor volume, and a higher accumulation of platinum in tumors of mice treated with the cisplatin-DCC conjugate in comparison to those treated by free cisplatin.

Návaznosti

GA16-05961S, projekt VaV

Název: Pokročilé nosiče platinových léčiv

Investor: Grantová agentura ČR, Pokročilé nosiče platinových léčiv

LM2015085, projekt VaV

Název: CERIT Scientific Cloud (Akronym: CERIT-SC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CERIT Scientific Cloud

MUNI/A/1255/2018, interní kód MU

Název: Kardiovaskulární systém a jeho regulace a dysregulace pod vlivem farmak (Akronym: KAREDYSFAR)

Investor: Masarykova univerzita, Kardiovaskulární systém a jeho regulace a dysregulace pod vlivem farmak, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

MUNI/A/1553/2018, interní kód MU

Název: Genetické, environmentální a tkáňové charakteristiky vybraných patologických stavů a nemocí (Akronym: genetika; stres; biomateriály; tkáňové kultury)

Investor: Masarykova univerzita, Genetické, environmentální a tkáňové charakteristiky vybraných patologických stavů a nemocí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

MUNSTER, Lukas; Michaela FOJTŮ; Zdenka CAPAKOVA; Tomáš VACULOVIČ; Michaela TVRDOŇOVÁ; Ivo KURITKA; Michal MASAŘÍK a Jan VICHA. Selectively Oxidized Cellulose with Adjustable Molecular Weight for Controlled Release of Platinum Anticancer Drugs. Biomacromolecules. Washington: American Chemical Society, 2019, roč. 20, č. 4, s. 1623-1634. ISSN 1525-7797. Dostupné z: https://doi.org/10.1021/acs.biomac.8b01807.

@article{1582276,
   author = {Munster, Lukas and Fojtů, Michaela and Capakova, Zdenka and Vaculovič, Tomáš and Tvrdoňová, Michaela and Kuritka, Ivo and Masařík, Michal and Vicha, Jan},
   article_location = {Washington},
   article_number = {4},
   doi = {https://doi.org/10.1021/acs.biomac.8b01807},
   keywords = {NMR CHEMICAL-SHIFTS; DENSITY-FUNCTIONAL THEORY; RELATIVISTIC DOUBLE-ZETA; HYALURONIC-ACID; DIALDEHYDE CELLULOSE; TRIPLE-ZETA; BASIS-SETS; PT-195 NMR; CISPLATIN; PERIODATE},
   language = {eng},
   issn = {1525-7797},
   journal = {Biomacromolecules},
   title = {Selectively Oxidized Cellulose with Adjustable Molecular Weight for Controlled Release of Platinum Anticancer Drugs},
   url = {http://dx.doi.org/10.1021/acs.biomac.8b01807},
   volume = {20},
   year = {2019}
}

TY  - JOUR
ID  - 1582276
AU  - Munster, Lukas - Fojtů, Michaela - Capakova, Zdenka - Vaculovič, Tomáš - Tvrdoňová, Michaela - Kuritka, Ivo - Masařík, Michal - Vicha, Jan
PY  - 2019
TI  - Selectively Oxidized Cellulose with Adjustable Molecular Weight for Controlled Release of Platinum Anticancer Drugs
JF  - Biomacromolecules
VL  - 20
IS  - 4
SP  - 1623-1634
EP  - 1623-1634
PB  - American Chemical Society
SN  - 15257797
KW  - NMR CHEMICAL-SHIFTS
KW  - DENSITY-FUNCTIONAL THEORY
KW  - RELATIVISTIC DOUBLE-ZETA
KW  - HYALURONIC-ACID
KW  - DIALDEHYDE CELLULOSE
KW  - TRIPLE-ZETA
KW  - BASIS-SETS
KW  - PT-195 NMR
KW  - CISPLATIN
KW  - PERIODATE
UR  - http://dx.doi.org/10.1021/acs.biomac.8b01807
L2  - http://dx.doi.org/10.1021/acs.biomac.8b01807
N2  - The synthesis of selectively oxidized cellulose, 2,3-dicarboxycellulose (DCC), is optimized for preparation of highly oxidized material for biological applications, which includes control over the molecular weight of the product during its synthesis. Conjugates of DCC and cisplatin simultaneously offer a very high drug binding efficiency (>90%) and drug loading capacity (up to 50 wt %), while retaining good aqueous solubility. The adjustable molecular weight of the DCC together with variances in drug feeding ratio allows to optimize cisplatin release profiles from delayed (<2% of cisplatin released during 6 h) to classical burst release with more than 60% of cisplatin released after 24 h. The release rates are also pH-dependent (up to 2 times faster release at pH 5.5 than at pH 7.4), which allows to exploit the acidic nature of tumor microenvironment. Extensive in vitro studies were performed on eight different cell lines for two cisplatin-DCC conjugates with different release profiles. In comparison with free cisplatin, both cisplatin-DCC conjugates demonstrated considerably lower cytotoxicity toward healthy cells. Conjugates with burst release profiles were found more effective against prostate cell lines, while DCC conjugates with slower release were more cytotoxic against ovarian and lung carcinoma cell lines. In vivo studies indicated a significantly longer survival rate, a reduction in tumor volume, and a higher accumulation of platinum in tumors of mice treated with the cisplatin-DCC conjugate in comparison to those treated by free cisplatin.
ER  -

MUNSTER, Lukas; Michaela FOJTŮ; Zdenka CAPAKOVA; Tomáš VACULOVIČ; Michaela TVRDOŇOVÁ; Ivo KURITKA; Michal MASAŘÍK a Jan VICHA. Selectively Oxidized Cellulose with Adjustable Molecular Weight for Controlled Release of Platinum Anticancer Drugs. \textit{Biomacromolecules}. Washington: American Chemical Society, 2019, roč.~20, č.~4, s.~1623-1634. ISSN~1525-7797. Dostupné z: https://doi.org/10.1021/acs.biomac.8b01807.

Přehled o publikaci