Bovine lactoferrin free of lipopolysaccharide can induce a
proinflammatory response of macrophages

J 2016

Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages

ZEMÁNKOVÁ, Naďa, Katarína CHLEBOVÁ, Jan MATIASOVIC, Jana PRODELALOVA, Jan GEBAUER et. al.

Základní údaje

Originální název

Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages

Autoři

ZEMÁNKOVÁ, Naďa (203 Česká republika, domácí), Katarína CHLEBOVÁ (703 Slovensko, domácí), Jan MATIASOVIC, Jana PRODELALOVA, Jan GEBAUER (203 Česká republika, domácí) a Martin FALDYNA (garant)

Vydání

BMC Veterinary Research, London, BMC, 2016, 1746-6148

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 1.750

Kód RIV

RIV/00216224:14310/16:00111639

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1186/s12917-016-0878-2

UT WoS

000387286300001

Klíčová slova anglicky

Inflammatory cytokines; LPS; NF kappa B; NIK; TLR4

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 5. 2020 10:24, Mgr. Marie Šípková, DiS.

Anotace

V originále

Background: Lactoferrin (LF) is an 80 kDa glycoprotein which is known for its effects against bacteria, viruses and other pathogens. It also has a high potential in nutrition therapy and welfare of people and a variety of animals, including piglets. The ability to bind lipopolysaccharide (LPS) is one of the described anti-inflammatory mechanisms of LF. Previous studies suggested that cells can be stimulated even by LPS-free LF. Therefore, the aim of our study was to bring additional information about this possibility. Porcine monocyte derived macrophages (MDMF) and human embryonic kidney (HEK) cells were stimulated with unpurified LF in complex with LPS and with purified LF without bound LPS. Results: Both cell types were stimulated with unpurified as well as purified LF. On the other hand, neither HEK0 cells not expressing any TLR nor HEK4a cells transfected with TLR4 produced any pro-inflammatory cytokine transcripts after stimulation with purified LF. This suggests that purified LF without LPS stimulates cells via another receptor than TLR4. An alternative, TLR4-independent, pathway was further confirmed by analyses of the NF-kappa-B- inducing kinase (NIK) activation. Western blot analyses showed NIK which activates different NF kappa B subunits compared to LF-LPS signaling via TLR4. Though, this confirmed an alternative pathway which is used by the purified LF free of LPS. This stimulation of MDMF led to low, but significant amounts of pro-inflammatory cytokines, which can be considered as a positive stimulation of the immune system. Conclusion: Our results suggest that LF's ability is not only to bind LPS, but LF itself may be a stimulant of pro-inflammatory pathways.

Citovat

ZEMÁNKOVÁ, Naďa, Katarína CHLEBOVÁ, Jan MATIASOVIC, Jana PRODELALOVA, Jan GEBAUER a Martin FALDYNA. Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages. BMC Veterinary Research. London: BMC, 2016, roč. 12, č. 251, s. 1-9. ISSN 1746-6148. Dostupné z: https://dx.doi.org/10.1186/s12917-016-0878-2.

@article{1590616,
   author = {Zemánková, Naďa and Chlebová, Katarína and Matiasovic, Jan and Prodelalova, Jana and Gebauer, Jan and Faldyna, Martin},
   article_location = {London},
   article_number = {251},
   doi = {http://dx.doi.org/10.1186/s12917-016-0878-2},
   keywords = {Inflammatory cytokines; LPS; NF kappa B; NIK; TLR4},
   language = {eng},
   issn = {1746-6148},
   journal = {BMC Veterinary Research},
   title = {Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages},
   url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103330/pdf/12917_2016_Article_878.pdf},
   volume = {12},
   year = {2016}
}

TY  - JOUR
ID  - 1590616
AU  - Zemánková, Naďa - Chlebová, Katarína - Matiasovic, Jan - Prodelalova, Jana - Gebauer, Jan - Faldyna, Martin
PY  - 2016
TI  - Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages
JF  - BMC Veterinary Research
VL  - 12
IS  - 251
SP  - 1-9
EP  - 1-9
PB  - BMC
SN  - 17466148
KW  - Inflammatory cytokines
KW  - LPS
KW  - NF kappa B
KW  - NIK
KW  - TLR4
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103330/pdf/12917_2016_Article_878.pdf
L2  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5103330/pdf/12917_2016_Article_878.pdf
N2  - Background: Lactoferrin (LF) is an 80 kDa glycoprotein which is known for its effects against bacteria, viruses and other pathogens. It also has a high potential in nutrition therapy and welfare of people and a variety of animals, including piglets. The ability to bind lipopolysaccharide (LPS) is one of the described anti-inflammatory mechanisms of LF. Previous studies suggested that cells can be stimulated even by LPS-free LF. Therefore, the aim of our study was to bring additional information about this possibility. Porcine monocyte derived macrophages (MDMF) and human embryonic kidney (HEK) cells were stimulated with unpurified LF in complex with LPS and with purified LF without bound LPS. Results: Both cell types were stimulated with unpurified as well as purified LF. On the other hand, neither HEK0 cells not expressing any TLR nor HEK4a cells transfected with TLR4 produced any pro-inflammatory cytokine transcripts after stimulation with purified LF. This suggests that purified LF without LPS stimulates cells via another receptor than TLR4. An alternative, TLR4-independent, pathway was further confirmed by analyses of the NF-kappa-B- inducing kinase (NIK) activation. Western blot analyses showed NIK which activates different NF kappa B subunits compared to LF-LPS signaling via TLR4. Though, this confirmed an alternative pathway which is used by the purified LF free of LPS. This stimulation of MDMF led to low, but significant amounts of pro-inflammatory cytokines, which can be considered as a positive stimulation of the immune system. Conclusion: Our results suggest that LF's ability is not only to bind LPS, but LF itself may be a stimulant of pro-inflammatory pathways.
ER  -

ZEMÁNKOVÁ, Naďa, Katarína CHLEBOVÁ, Jan MATIASOVIC, Jana PRODELALOVA, Jan GEBAUER a Martin FALDYNA. Bovine lactoferrin free of lipopolysaccharide can induce a proinflammatory response of macrophages. \textit{BMC Veterinary Research}. London: BMC, 2016, roč.~12, č.~251, s.~1-9. ISSN~1746-6148. Dostupné z: https://dx.doi.org/10.1186/s12917-016-0878-2.

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