Fast blood plasma separation device for point-of-care
applications

J 2018

Fast blood plasma separation device for point-of-care applications

ĎURČ, Pavol; František FORET a Petr KUBÁŇ

Základní údaje

Originální název

Fast blood plasma separation device for point-of-care applications

Autoři

ĎURČ, Pavol (703 Slovensko, domácí); František FORET (203 Česká republika) a Petr KUBÁŇ (203 Česká republika, garant)

Vydání

Talanta, Amsterdam, ELSEVIER SCIENCE BV, 2018, 0039-9140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 4.916

Kód RIV

RIV/00216224:14310/18:00111646

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.talanta.2018.02.004

UT WoS

000430645800008

EID Scopus

2-s2.0-85042222134

Klíčová slova anglicky

Blood plasma separation; Point of care analysis; Capillary electrophoresis; Alcohol intoxication; Metabolites; Methanol

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 12. 2019 16:59, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

In this work, a simple device for extremely fast separation of blood plasma from diluted whole blood was developed. The device accommodates an asymmetric polysulfone membrane/supporting membrane sandwich that allows collection of 10 mu L blood plasma into a narrow glass capillary in less than 10 s. The composition of diluent solution was optimized in order to achieve maximum recoveries for selected metabolites of alcohol intoxication. 5% solution of [tris(hydroxymethyl)methylamino] propanesulfonic acid provided recoveries of formate, oxalate and glycolate close to 100% and only moderate erythrocyte lysis. Both charged and uncharged compounds from the whole blood samples can be analyzed in the separated blood plasma by capillary electrophoresis with contactless conductometric detection and spectrophotometry, respectively. The developed device might find wide application in on-site testing and point-of-care analysis, when only microliter volumes of whole blood are available.

Citovat

ĎURČ, Pavol; František FORET a Petr KUBÁŇ. Fast blood plasma separation device for point-of-care applications. Talanta. Amsterdam: ELSEVIER SCIENCE BV, 2018, roč. 183, JUN, s. 55-60. ISSN 0039-9140. Dostupné z: https://doi.org/10.1016/j.talanta.2018.02.004.

@article{1590677,
   author = {Ďurč, Pavol and Foret, František and Kubáň, Petr},
   article_location = {Amsterdam},
   article_number = {JUN},
   doi = {https://doi.org/10.1016/j.talanta.2018.02.004},
   keywords = {Blood plasma separation; Point of care analysis; Capillary electrophoresis; Alcohol intoxication; Metabolites; Methanol},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Fast blood plasma separation device for point-of-care applications},
   url = {https://www.sciencedirect.com/science/article/pii/S0039914018301127},
   volume = {183},
   year = {2018}
}

TY  - JOUR
ID  - 1590677
AU  - Ďurč, Pavol - Foret, František - Kubáň, Petr
PY  - 2018
TI  - Fast blood plasma separation device for point-of-care applications
JF  - Talanta
VL  - 183
IS  - JUN
SP  - 55-60
EP  - 55-60
PB  - ELSEVIER SCIENCE BV
SN  - 00399140
KW  - Blood plasma separation
KW  - Point of care analysis
KW  - Capillary electrophoresis
KW  - Alcohol intoxication
KW  - Metabolites
KW  - Methanol
UR  - https://www.sciencedirect.com/science/article/pii/S0039914018301127
L2  - https://www.sciencedirect.com/science/article/pii/S0039914018301127
N2  - In this work, a simple device for extremely fast separation of blood plasma from diluted whole blood was developed. The device accommodates an asymmetric polysulfone membrane/supporting membrane sandwich that allows collection of 10 mu L blood plasma into a narrow glass capillary in less than 10 s. The composition of diluent solution was optimized in order to achieve maximum recoveries for selected metabolites of alcohol intoxication. 5% solution of [tris(hydroxymethyl)methylamino] propanesulfonic acid provided recoveries of formate, oxalate and glycolate close to 100% and only moderate erythrocyte lysis. Both charged and uncharged compounds from the whole blood samples can be analyzed in the separated blood plasma by capillary electrophoresis with contactless conductometric detection and spectrophotometry, respectively. The developed device might find wide application in on-site testing and point-of-care analysis, when only microliter volumes of whole blood are available.
ER  -

ĎURČ, Pavol; František FORET a Petr KUBÁŇ. Fast blood plasma separation device for point-of-care applications. \textit{Talanta}. Amsterdam: ELSEVIER SCIENCE BV, 2018, roč.~183, JUN, s.~55-60. ISSN~0039-9140. Dostupné z: https://doi.org/10.1016/j.talanta.2018.02.004.

Přehled o publikaci