Dissemination and Comparison of Genetic Determinants of
mcr-Mediated Colistin Resistance in Enterobacteriaceae via
Retailed Raw Meat Products

J 2019

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

GELBÍČOVÁ, Tereza; Alžběta BARÁKOVÁ; Martina FLORIANOVÁ; Ivana JAMBOROVÁ; Markéta ZELENDOVÁ et al.

Základní údaje

Originální název

Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products

Autoři

GELBÍČOVÁ, Tereza; Alžběta BARÁKOVÁ; Martina FLORIANOVÁ; Ivana JAMBOROVÁ; Markéta ZELENDOVÁ; Lucie POSPÍŠILOVÁ; Ivana KOLÁČKOVÁ a Renata KARPÍŠKOVÁ

Vydání

Frontiers in Microbiology, Lausanne, FRONTIERS MEDIA SA, 2019, 1664-302X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 4.236

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00111721

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

colistin; mcr gene; resistance; Enterobacteriaceae; meat; retail

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2022 13:09, Mgr. Tereza Miškechová

Anotace

V originále

The global food chain may significantly promote the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae with mcr-mediated colistin (CT) resistance in retail meat of different origins. Bacteria of the Enterobacteriaceae family carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of the meat and liver of chicken and other poultry and of pork and beef were negative for the presence of bacteria carrying the mcr-1 to mcr-5 genes. A huge number of isolates belonging to Escherchia coli (n = 54), Klebsiella pneumoniae (n = 6), and Citrobacter braakii (n = 1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, and IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czechia, Germany, Poland, and Brazil. Our findings indicate highly probable interspecies transfer of IncX4 and IncI2 plasmids within one meat sample. The co-resistance of plasmid-mediated CT resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multidrug-resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying the mcr-1 gene in retail meat reflects the need for Europe-wide monitoring of mcr-mediated CT resistance throughout the whole food chain.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

GELBÍČOVÁ, Tereza; Alžběta BARÁKOVÁ; Martina FLORIANOVÁ; Ivana JAMBOROVÁ; Markéta ZELENDOVÁ; Lucie POSPÍŠILOVÁ; Ivana KOLÁČKOVÁ a Renata KARPÍŠKOVÁ. Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products. Frontiers in Microbiology. Lausanne: FRONTIERS MEDIA SA, 2019, roč. 10, DEC 12 2019, s. 1-10. ISSN 1664-302X. Dostupné z: https://doi.org/10.3389/fmicb.2019.02824.

@article{1592796,
   author = {Gelbíčová, Tereza and Baráková, Alžběta and Florianová, Martina and Jamborová, Ivana and Zelendová, Markéta and Pospíšilová, Lucie and Koláčková, Ivana and Karpíšková, Renata},
   article_location = {Lausanne},
   article_number = {DEC 12 2019},
   doi = {https://doi.org/10.3389/fmicb.2019.02824},
   keywords = {colistin; mcr gene; resistance; Enterobacteriaceae; meat; retail},
   language = {eng},
   issn = {1664-302X},
   journal = {Frontiers in Microbiology},
   title = {Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products},
   url = {https://doi.org/10.3389/fmicb.2019.02824},
   volume = {10},
   year = {2019}
}

TY  - JOUR
ID  - 1592796
AU  - Gelbíčová, Tereza - Baráková, Alžběta - Florianová, Martina - Jamborová, Ivana - Zelendová, Markéta - Pospíšilová, Lucie - Koláčková, Ivana - Karpíšková, Renata
PY  - 2019
TI  - Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products
JF  - Frontiers in Microbiology
VL  - 10
IS  - DEC 12 2019
SP  - 1-10
EP  - 1-10
PB  - FRONTIERS MEDIA SA
SN  - 1664302X
KW  - colistin
KW  - mcr gene
KW  - resistance
KW  - Enterobacteriaceae
KW  - meat
KW  - retail
UR  - https://doi.org/10.3389/fmicb.2019.02824
L2  - https://doi.org/10.3389/fmicb.2019.02824
N2  - The global food chain may significantly promote the dissemination of bacteria resistant to antibiotics around the world. This study was aimed at determining the prevalence and genetic characteristics of Enterobacteriaceae with mcr-mediated colistin (CT) resistance in retail meat of different origins. Bacteria of the Enterobacteriaceae family carrying the mcr-1 gene were detected in 21% (18/86) of the examined samples, especially in turkey meat and liver originating from EU and non-EU countries (19%) and in rabbit meat imported from China (2%). The examined samples of the meat and liver of chicken and other poultry and of pork and beef were negative for the presence of bacteria carrying the mcr-1 to mcr-5 genes. A huge number of isolates belonging to Escherchia coli (n = 54), Klebsiella pneumoniae (n = 6), and Citrobacter braakii (n = 1) carrying the mcr-1 gene were obtained. Despite the high heterogeneity of the tested isolates, the mcr-1 gene was localized on only three types of plasmids (IncX4, IncHI2, and IncI2). The most frequent type of plasmid was IncX4, which carried the mcr-1 gene in 77% of E. coli and K. pneumoniae isolates from turkey meat and liver samples from the Czechia, Germany, Poland, and Brazil. Our findings indicate highly probable interspecies transfer of IncX4 and IncI2 plasmids within one meat sample. The co-resistance of plasmid-mediated CT resistance encoded by the mcr-1 and ESBL genes was detected in 18% of the isolates. Another noteworthy finding was the fosA3 gene coding for fosfomycin resistance in a multidrug-resistant isolate of E. coli from rabbit meat imported from China. The observed high level of Enterobacteriaceae with plasmids carrying the mcr-1 gene in retail meat reflects the need for Europe-wide monitoring of mcr-mediated CT resistance throughout the whole food chain.
ER  -

GELBÍČOVÁ, Tereza; Alžběta BARÁKOVÁ; Martina FLORIANOVÁ; Ivana JAMBOROVÁ; Markéta ZELENDOVÁ; Lucie POSPÍŠILOVÁ; Ivana KOLÁČKOVÁ a Renata KARPÍŠKOVÁ. Dissemination and Comparison of Genetic Determinants of mcr-Mediated Colistin Resistance in Enterobacteriaceae via Retailed Raw Meat Products. \textit{Frontiers in Microbiology}. Lausanne: FRONTIERS MEDIA SA, 2019, roč.~10, DEC 12 2019, s.~1-10. ISSN~1664-302X. Dostupné z: https://doi.org/10.3389/fmicb.2019.02824.

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