Development of a Magnetic Electrochemical Bar Code Array for
Point Mutation Detection in the H5N1 Neuraminidase Gene

J 2013

Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene

KREJCOVA, Ludmila, David HYNEK, Pavel KOPEL, Miguel Angel Merlos RODRIGO, Vojtech ADAM et. al.

Základní údaje

Originální název

Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene

Autoři

KREJCOVA, Ludmila, David HYNEK, Pavel KOPEL, Miguel Angel Merlos RODRIGO, Vojtech ADAM, Jaromir HUBALEK, Petr BABULA, Libuše TRNKOVÁ (203 Česká republika, domácí) a Rene KIZEK

Vydání

VIRUSES-BASEL, Basel, MDPI AG, 2013, 1999-4915

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 3.279

Kód RIV

RIV/00216224:14310/13:00107096

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/v5071719

UT WoS

000322172200009

Klíčová slova anglicky

voltammetry; highly pathogenic influenza; antiviral resistance; paramagnetic particles; hybridization; quantum dots; automated separation; electrochemistry

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 12. 2019 14:51, Mgr. Marie Šípková, DiS.

Anotace

V originále

Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads (R) with covalently bound oligo (dT)(25) were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual probes hybridized to target molecules specifically with efficiency higher than 60%. The obtained signals identified mutations present in the sequence. Suggested experimental procedure allows obtaining further information from the redox signals of nucleic acids. Moreover, the used biosensor exhibits sequence specificity and low limits of detection of subnanogram quantities of target nucleic acids.

Návaznosti

CZ.1.07/2.4.00/31.0023, interní kód MU

Název: Partnerská siť centra excelentního bionanotechnologického výzkumu (Akronym: NanoBioMetalTechNet)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Partnerská siť centra excelentního bionanotechnologického výzkumu, 2.2 Vysokoškolské vzdělávání

Citovat

KREJCOVA, Ludmila, David HYNEK, Pavel KOPEL, Miguel Angel Merlos RODRIGO, Vojtech ADAM, Jaromir HUBALEK, Petr BABULA, Libuše TRNKOVÁ a Rene KIZEK. Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene. VIRUSES-BASEL. Basel: MDPI AG, 2013, roč. 5, č. 7, s. 1719-1739. ISSN 1999-4915. Dostupné z: https://dx.doi.org/10.3390/v5071719.

@article{1592844,
   author = {Krejcova, Ludmila and Hynek, David and Kopel, Pavel and Rodrigo, Miguel Angel Merlos and Adam, Vojtech and Hubalek, Jaromir and Babula, Petr and Trnková, Libuše and Kizek, Rene},
   article_location = {Basel},
   article_number = {7},
   doi = {http://dx.doi.org/10.3390/v5071719},
   keywords = {voltammetry; highly pathogenic influenza; antiviral resistance; paramagnetic particles; hybridization; quantum dots; automated separation; electrochemistry},
   language = {eng},
   issn = {1999-4915},
   journal = {VIRUSES-BASEL},
   title = {Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene},
   url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738958/pdf/viruses-05-01719.pdf},
   volume = {5},
   year = {2013}
}

TY  - JOUR
ID  - 1592844
AU  - Krejcova, Ludmila - Hynek, David - Kopel, Pavel - Rodrigo, Miguel Angel Merlos - Adam, Vojtech - Hubalek, Jaromir - Babula, Petr - Trnková, Libuše - Kizek, Rene
PY  - 2013
TI  - Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene
JF  - VIRUSES-BASEL
VL  - 5
IS  - 7
SP  - 1719-1739
EP  - 1719-1739
PB  - MDPI AG
SN  - 19994915
KW  - voltammetry
KW  - highly pathogenic influenza
KW  - antiviral resistance
KW  - paramagnetic particles
KW  - hybridization
KW  - quantum dots
KW  - automated separation
KW  - electrochemistry
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738958/pdf/viruses-05-01719.pdf
L2  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738958/pdf/viruses-05-01719.pdf
N2  - Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads (R) with covalently bound oligo (dT)(25) were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual probes hybridized to target molecules specifically with efficiency higher than 60%. The obtained signals identified mutations present in the sequence. Suggested experimental procedure allows obtaining further information from the redox signals of nucleic acids. Moreover, the used biosensor exhibits sequence specificity and low limits of detection of subnanogram quantities of target nucleic acids.
ER  -

KREJCOVA, Ludmila, David HYNEK, Pavel KOPEL, Miguel Angel Merlos RODRIGO, Vojtech ADAM, Jaromir HUBALEK, Petr BABULA, Libuše TRNKOVÁ a Rene KIZEK. Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene. \textit{VIRUSES-BASEL}. Basel: MDPI AG, 2013, roč.~5, č.~7, s.~1719-1739. ISSN~1999-4915. Dostupné z: https://dx.doi.org/10.3390/v5071719.

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