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@article{1592978, author = {Mazur, Antonina Joanna and Radaszkiewicz, Tomasz Witold and Makowiecka, Aleksandra and MalickaandBlaszkiewicz, Maria and Mannherz, Hans Georg and Nowak, Dorota}, article_location = {Jena}, article_number = {1}, doi = {http://dx.doi.org/10.1016/j.ejcb.2015.11.001}, keywords = {Gelsolin; actbl2; LamR; hnRNP U; Nestin; beta Tubulin}, language = {eng}, issn = {0171-9335}, journal = {European Journal of Cell Biology}, title = {Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and beta-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry}, url = {https://www.sciencedirect.com/science/article/pii/S0171933515300182?via%3Dihub}, volume = {95}, year = {2016} }
TY - JOUR ID - 1592978 AU - Mazur, Antonina Joanna - Radaszkiewicz, Tomasz Witold - Makowiecka, Aleksandra - Malicka-Blaszkiewicz, Maria - Mannherz, Hans Georg - Nowak, Dorota PY - 2016 TI - Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and beta-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry JF - European Journal of Cell Biology VL - 95 IS - 1 SP - 26-41 EP - 26-41 PB - Elsevier GMBH SN - 01719335 KW - Gelsolin KW - actbl2 KW - LamR KW - hnRNP U KW - Nestin KW - beta Tubulin UR - https://www.sciencedirect.com/science/article/pii/S0171933515300182?via%3Dihub L2 - https://www.sciencedirect.com/science/article/pii/S0171933515300182?via%3Dihub N2 - Gelsolin, a multifunctional actin binding protein, plays a not yet fully understood role in tumorigenesis. Therefore the goal of this study was to identify additional molecular partners of gelsolin in human melanoma cells, separately in the cytoplasmic compartment and cell nuclei. For this purpose we performed immunoprecipitation experiments based on a modified protocol followed by mass spectrometry. The obtained results were confirmed by Western blot analysis, proximity ligation assays and confocal microscopy. As expected gelsolin interacted with actin, in particular we demonstrate its interaction with cytoplasmic beta and gamma actins, and a newly discovered actin isoform, actbl2. As new gelsolin-interacting partners we identified the ribosomal protein Rpsa, also known as a non-integrin laminin receptor (LamR), and the heterogeneous nuclear ribonucleoprotein hnRNP U. Our data furthermore indicate that gelsolin interacts with particular components of the three cytoskeleton systems: nestin (intermediate filaments), Arp3 (actin cytoskeleton) and beta-tubulin (microtubules). We also report for the first time that gelsolin is a constituent of midbodies, a tubulin containing structure formed at the end of cytokinesis. ER -
MAZUR, Antonina Joanna, Tomasz Witold RADASZKIEWICZ, Aleksandra MAKOWIECKA, Maria MALICKA-BLASZKIEWICZ, Hans Georg MANNHERZ a Dorota NOWAK. Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and beta-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry. \textit{European Journal of Cell Biology}. Jena: Elsevier GMBH, 2016, roč.~95, č.~1, s.~26-41. ISSN~0171-9335. Dostupné z: https://dx.doi.org/10.1016/j.ejcb.2015.11.001.
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