Full genome sequences and molecular characterization of
tick-borne encephalitis virus strains isolated from human
patients

J 2015

Full genome sequences and molecular characterization of tick-borne encephalitis virus strains isolated from human patients

FORMANOVÁ, Petra; Jiri CERNY; Barbora Cerna BOLFIKOVA; James J VALDES; Irina KOZLOVA et al.

Základní údaje

Originální název

Full genome sequences and molecular characterization of tick-borne encephalitis virus strains isolated from human patients

Autoři

FORMANOVÁ, Petra; Jiri CERNY; Barbora Cerna BOLFIKOVA; James J VALDES; Irina KOZLOVA; Yuri DZHIOEV a Daniel RŮŽEK

Vydání

Ticks and Tick-borne Diseases, MUNICH, ELSEVIER GMBH, 2015, 1877-959X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30303 Infectious Diseases

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.690

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00111738

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Tick-borne encephalitis virus; Tick-borne encephalitis; Genome analysis; Human patients

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 12. 2019 20:26, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Tick-borne encephalitis virus (TBEV) causes tick-borne encephalitis (TBE), one of the most important human neuroinfections across Eurasia. Up to date, only three full genome sequences of human European TBEV isolates are available, mostly due to difficulties with isolation of the virus from human patients. Here we present full genome characterization of an additional five low-passage TBEV strains isolated from human patients with severe forms of TBE. These strains were isolated in 1953 within Central Bohemia in the former Czechoslovakia, and belong to the historically oldest human TBEV isolates in Europe. We demonstrate here that all analyzed isolates are distantly phylogenetically related, indicating that the emergence of TBE in Central Europe was not caused by one predominant strain, but rather a pool of distantly related TBEV strains. Nucleotide identity between individual sequenced TBEV strains ranged from 97.5% to 99.6% and all strains shared large deletions in the 3' non-coding region, which has been recently suggested to be an important determinant of virulence. The number of unique amino acid substitutions varied from 3 to 9 in individual isolates, but no characteristic amino acid substitution typical exclusively for all human TBEV isolates was identified when compared to the isolates from ticks. We did, however, correlate that the exploration of the TBEV envelope glycoprotein by specific antibodies were in close proximity to these unique amino acid substitutions. Taken together, we report here the largest number of patient-derived European TBEV full genome sequences to date and provide a platform for further studies on evolution of TBEV since the first emergence of human TBE in Europe.

Citovat

FORMANOVÁ, Petra; Jiri CERNY; Barbora Cerna BOLFIKOVA; James J VALDES; Irina KOZLOVA; Yuri DZHIOEV a Daniel RŮŽEK. Full genome sequences and molecular characterization of tick-borne encephalitis virus strains isolated from human patients. Ticks and Tick-borne Diseases. MUNICH: ELSEVIER GMBH, 2015, roč. 6, č. 1, s. 38-46. ISSN 1877-959X. Dostupné z: https://doi.org/10.1016/j.ttbdis.2014.09.002.

@article{1593024,
   author = {Formanová, Petra and Cerny, Jiri and Bolfikova, Barbora Cerna and Valdes, James J and Kozlova, Irina and Dzhioev, Yuri and Růžek, Daniel},
   article_location = {MUNICH},
   article_number = {1},
   doi = {https://doi.org/10.1016/j.ttbdis.2014.09.002},
   keywords = {Tick-borne encephalitis virus; Tick-borne encephalitis; Genome analysis; Human patients},
   language = {eng},
   issn = {1877-959X},
   journal = {Ticks and Tick-borne Diseases},
   title = {Full genome sequences and molecular characterization of tick-borne encephalitis virus strains isolated from human patients},
   url = {https://www.sciencedirect.com/science/article/pii/S1877959X1400185X},
   volume = {6},
   year = {2015}
}

TY  - JOUR
ID  - 1593024
AU  - Formanová, Petra - Cerny, Jiri - Bolfikova, Barbora Cerna - Valdes, James J - Kozlova, Irina - Dzhioev, Yuri - Růžek, Daniel
PY  - 2015
TI  - Full genome sequences and molecular characterization of tick-borne encephalitis virus strains isolated from human patients
JF  - Ticks and Tick-borne Diseases
VL  - 6
IS  - 1
SP  - 38-46
EP  - 38-46
PB  - ELSEVIER GMBH
SN  - 1877959X
KW  - Tick-borne encephalitis virus
KW  - Tick-borne encephalitis
KW  - Genome analysis
KW  - Human patients
UR  - https://www.sciencedirect.com/science/article/pii/S1877959X1400185X
L2  - https://www.sciencedirect.com/science/article/pii/S1877959X1400185X
N2  - Tick-borne encephalitis virus (TBEV) causes tick-borne encephalitis (TBE), one of the most important human neuroinfections across Eurasia. Up to date, only three full genome sequences of human European TBEV isolates are available, mostly due to difficulties with isolation of the virus from human patients. Here we present full genome characterization of an additional five low-passage TBEV strains isolated from human patients with severe forms of TBE. These strains were isolated in 1953 within Central Bohemia in the former Czechoslovakia, and belong to the historically oldest human TBEV isolates in Europe. We demonstrate here that all analyzed isolates are distantly phylogenetically related, indicating that the emergence of TBE in Central Europe was not caused by one predominant strain, but rather a pool of distantly related TBEV strains. Nucleotide identity between individual sequenced TBEV strains ranged from 97.5% to 99.6% and all strains shared large deletions in the 3' non-coding region, which has been recently suggested to be an important determinant of virulence. The number of unique amino acid substitutions varied from 3 to 9 in individual isolates, but no characteristic amino acid substitution typical exclusively for all human TBEV isolates was identified when compared to the isolates from ticks. We did, however, correlate that the exploration of the TBEV envelope glycoprotein by specific antibodies were in close proximity to these unique amino acid substitutions. Taken together, we report here the largest number of patient-derived European TBEV full genome sequences to date and provide a platform for further studies on evolution of TBEV since the first emergence of human TBE in Europe.
ER  -

FORMANOVÁ, Petra; Jiri CERNY; Barbora Cerna BOLFIKOVA; James J VALDES; Irina KOZLOVA; Yuri DZHIOEV a Daniel RŮŽEK. Full genome sequences and molecular characterization of tick-borne encephalitis virus strains isolated from human patients. \textit{Ticks and Tick-borne Diseases}. MUNICH: ELSEVIER GMBH, 2015, roč.~6, č.~1, s.~38-46. ISSN~1877-959X. Dostupné z: https://doi.org/10.1016/j.ttbdis.2014.09.002.

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