Analysis of selected bile acids in saliva by high-performance
liquid chromatography-mass spectrometry as a potential new
non-invasive diagnostic method for Barrett ’s esophagus

a 2019

Analysis of selected bile acids in saliva by high-performance liquid chromatography-mass spectrometry as a potential new non-invasive diagnostic method for Barrett ’s esophagus

KONEČNÝ, Štefan, Petr KUBÁŇ, Pavol ĎURČ, Jiří DOLINA, Věra DOSEDĚLOVÁ et. al.

Základní údaje

Originální název

Analysis of selected bile acids in saliva by high-performance liquid chromatography-mass spectrometry as a potential new non-invasive diagnostic method for Barrett ’s esophagus

Autoři

KONEČNÝ, Štefan (703 Slovensko, garant, domácí), Petr KUBÁŇ (203 Česká republika, domácí), Pavol ĎURČ (703 Slovensko, domácí), Jiří DOLINA (203 Česká republika, domácí), Věra DOSEDĚLOVÁ (203 Česká republika, domácí), M. HIMMELSBACH (40 Rakousko) a W. BUCHBERG (40 Rakousko)

Vydání

27th United European Gastroenterology Week Barcelona 2019, 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30219 Gastroenterology and hepatology

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.549

Kód RIV

RIV/00216224:14110/19:00108553

Organizační jednotka

Lékařská fakulta

ISSN

Klíčová slova anglicky

bile acids; Barrett's oesophagus; noninvasive analysis; saliva

Štítky

rivok

Příznaky

Mezinárodní význam

Změněno: 14. 4. 2020 15:48, Mgr. Tereza Miškechová

Anotace

V originále

Introduction: Barrett’s esophagus (BE) is defined as the replacement of squamous epithelium in the distal esophagus with metaplastic intestinal columnar epithelium and develops because of chronic inflammation resulting from gastroesophageal reflux disease. BE is associated with an increased risk of developing esophageal adenocarcinoma (EAC). Experimental, clinical and immunohistochemical studies show that one of the factors in the pathogenesis of esophageal injury, BE and EAC is duodenal reflux and the associated synergistic damage to the esophagus by bile acid (BA) and gastric acid reflux. Oxidative stress secondary to bile acids (BAs) exposure has been associated with metaplastic degeneration of normal esophageal mucosa into BE cells and eventually EAC. At the current time, a diagnosis of Barrett’s esophagus can only be made using endoscopy and detecting a change in the lining of the esophagus. The definitive diagnosis of Barrett’s esophagus requires biopsy confirmation of the change in the lining of the esophagus. The Prague classification system is universally accepted standardized endoscopic grading system for BE and uses the “C” value as the “circumferential extent” and the “M” value as “maximal extent” of BE above the gastroesophageal junction in centimeters for endoscopic standardization of BE lengths. Aims & Methods: We studied the content of selected BAs in saliva samples in a cohort of 15 patients with endoscopically and histologically proven BE (Prague Classification C: 1-10, M: 2-13) and a control group consisting of10 subjects without known medical history or treatment for GERD or BE at least in the last 5 years. Concentration of major salivary BAs (glycocholic acid, glycodeoxycholic acid, and glycochenodeoxycholic acid) in saliva were analyzed using high-performance liquid chromatography-mass spectrometry (HPLC-MS) detection. Saliva samples were collected from healthy individuals and patients with BE during the day, but all subjects were asked not to eat, drink nor brush their teeth at least 3 hours before sampling. Afterwards were samples stored at -80 °C. Before the measurement saliva samples were let to thaw at room temperature. After that 900 ul of MeOH was added to 300 ul of saliva sample for protein precipitation. This mixture was vortexed, sonicated and centrifugated and 1 mL of the supernatant was transferred to a new vial. Supernatant was dried under stream of nitrogen. Dried sample was re-dissolved in 300 ul of 80% MeOH, vortexed, sonicated and the supernatant was analyzed by HPLC-MS. The analysis was completed under 4 minutes. Results: We compared the concentrations of selected BAs in both groups of patients, and we found significantly increased concentrations of glycochenodeoxycholic acid (p<0.01) in the group of patients with BE. Significantly better statistical differentiation of samples was observed by using principal component analysis, especially in patients with high C and M values of BE length by Prague classification. Conclusion: Analysis of selected bile acids in saliva by HPLC-MS appears to be a potential new non-invasive diagnostic method for detection of Barrett’s esophagus and for estimation of its severity. HPLC-MS is a highly sensitive method to measure low amounts of bile acids in human saliva. The analysis is fast, but the sample preparation is more complicated. Further refinement of this new diagnostic method is needed.

Návaznosti

NV17-31945A, projekt VaV

Název: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu

Citovat

KONEČNÝ, Štefan, Petr KUBÁŇ, Pavol ĎURČ, Jiří DOLINA, Věra DOSEDĚLOVÁ, M. HIMMELSBACH a W. BUCHBERG. Analysis of selected bile acids in saliva by high-performance liquid chromatography-mass spectrometry as a potential new non-invasive diagnostic method for Barrett ’s esophagus. In 27th United European Gastroenterology Week Barcelona 2019. 2019. ISSN 2050-6414.

@proceedings{1595744,
   author = {Konečný, Štefan and Kubáň, Petr and Ďurč, Pavol and Dolina, Jiří and Dosedělová, Věra and Himmelsbach, M. and Buchberg, W.},
   booktitle = {27th United European Gastroenterology Week Barcelona 2019},
   keywords = {bile acids; Barrett's oesophagus; noninvasive analysis; saliva},
   language = {eng},
   title = {Analysis of selected bile acids in saliva by high-performance liquid chromatography-mass spectrometry as a potential new non-invasive diagnostic method for Barrett ’s esophagus},
   url = {https://www.ueg.eu/fileadmin/user_upload/documents/week2019/UEG2019_Abstract_book.pdf},
   year = {2019}
}

TY  - CONF
ID  - 1595744
AU  - Konečný, Štefan - Kubáň, Petr - Ďurč, Pavol - Dolina, Jiří - Dosedělová, Věra - Himmelsbach, M. - Buchberg, W.
PY  - 2019
TI  - Analysis of selected bile acids in saliva by high-performance liquid chromatography-mass spectrometry as a potential new non-invasive diagnostic method for Barrett ’s esophagus
KW  - bile acids
KW  - Barrett's oesophagus
KW  - noninvasive analysis
KW  - saliva
UR  - https://www.ueg.eu/fileadmin/user_upload/documents/week2019/UEG2019_Abstract_book.pdf
L2  - https://www.ueg.eu/fileadmin/user_upload/documents/week2019/UEG2019_Abstract_book.pdf
N2  - Introduction: Barrett’s esophagus (BE) is defined as the replacement of squamous epithelium in the distal esophagus with metaplastic intestinal columnar epithelium and develops because of chronic inflammation resulting from gastroesophageal reflux disease. BE is associated with an increased risk of developing esophageal adenocarcinoma (EAC). Experimental, clinical and immunohistochemical studies show that one of the factors in the pathogenesis of esophageal injury, BE and EAC is duodenal reflux and the associated synergistic damage to the esophagus by bile acid (BA) and gastric acid reflux. Oxidative stress secondary to bile acids (BAs) exposure has been associated with metaplastic degeneration of normal esophageal mucosa into BE cells and eventually EAC. At the current time, a diagnosis of Barrett’s esophagus can only be made using endoscopy and detecting a change in the lining of the esophagus. The definitive diagnosis of Barrett’s esophagus requires biopsy confirmation of the change in the lining of the esophagus. The Prague classification system is universally accepted standardized endoscopic grading system for BE and uses the “C” value as the “circumferential extent” and the “M” value as “maximal extent” of BE above the gastroesophageal junction in centimeters for endoscopic standardization of BE lengths. Aims & Methods: We studied the content of selected BAs in saliva samples in a cohort of 15 patients with endoscopically and histologically proven BE (Prague Classification C: 1-10, M: 2-13) and a control group consisting of10 subjects without known medical history or treatment for GERD or BE at least in the last 5 years. Concentration of major salivary BAs (glycocholic acid, glycodeoxycholic acid, and glycochenodeoxycholic acid) in saliva were analyzed using high-performance liquid chromatography-mass spectrometry (HPLC-MS) detection. Saliva samples were collected from healthy individuals and patients with BE during the day, but all subjects were asked not to eat, drink nor brush their teeth at least 3 hours before sampling. Afterwards were samples stored at -80 °C. Before the measurement saliva samples were let to thaw at room temperature. After that 900 ul of MeOH was added to 300 ul of saliva sample for protein precipitation. This mixture was vortexed, sonicated and centrifugated and 1 mL of the supernatant was transferred to a new vial. Supernatant was dried under stream of nitrogen. Dried sample was re-dissolved in 300 ul of 80% MeOH, vortexed, sonicated and the supernatant was analyzed by HPLC-MS. The analysis was completed under 4 minutes. Results: We compared the concentrations of selected BAs in both groups of patients, and we found significantly increased concentrations of glycochenodeoxycholic acid (p<0.01) in the group of patients with BE. Significantly better statistical differentiation of samples was observed by using principal component analysis, especially in patients with high C and M values of BE length by Prague classification. Conclusion: Analysis of selected bile acids in saliva by HPLC-MS appears to be a potential new non-invasive diagnostic method for detection of Barrett’s esophagus and for estimation of its severity. HPLC-MS is a highly sensitive method to measure low amounts of bile acids in human saliva. The analysis is fast, but the sample preparation is more complicated. Further refinement of this new diagnostic method is needed.
ER  -

KONEČNÝ, Štefan, Petr KUBÁŇ, Pavol ĎURČ, Jiří DOLINA, Věra DOSEDĚLOVÁ, M. HIMMELSBACH a W. BUCHBERG. Analysis of selected bile acids in saliva by high-performance liquid chromatography-mass spectrometry as a potential new non-invasive diagnostic method for Barrett ’s esophagus. In \textit{27th United European Gastroenterology Week Barcelona 2019}. 2019. ISSN~2050-6414.

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