Ultrasound-assisted production of highly-purified beta-glucan
schizophyllan and characterization of its immune properties

J 2017

Ultrasound-assisted production of highly-purified beta-glucan schizophyllan and characterization of its immune properties

SMIRNOU, Dzianis; Petr KNOTEK; Kristina NEŠPOROVÁ; Daniela SMEJKALOVA; Vojtech PAVLIK et al.

Základní údaje

Originální název

Ultrasound-assisted production of highly-purified beta-glucan schizophyllan and characterization of its immune properties

Autoři

SMIRNOU, Dzianis; Petr KNOTEK; Kristina NEŠPOROVÁ; Daniela SMEJKALOVA; Vojtech PAVLIK; Lukas FRANKE a Vladimir VELEBNY

Vydání

Process Biochemistry, Oxford, ELSEVIER SCI LTD, 2017, 1359-5113

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.616

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/17:00111923

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Schizophyllan ultrasound; Downstream; Proteins; Endotoxins; Immune effect

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 1. 2020 16:15, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

In this work, we suggest procedure for manufacturing of highly pure schizophyllan (SPG) cleaved into shorter fragments by ultrasonication. The ultrasonicated SPG (uSPG) was prepared from Schizophyllum commune submerged cultivation in bioreactor, culture broth ultrasonication with 100 Ws/mL energy input, followed by depth filtration and ultrafiltration. The uSPG contained less than 0.3%w/w protein and 0.078 IU/mg endotoxin. Chemical composition and structure of the uSPG were analyzed by GC and NMR, and conformation of the molecules was documented by AFM. Apyrogenicity of uSPG was proved by MAT test. Immunomodulatory properties of native and denatured uSPG were studied in vitro with human whole blood. The LPS-spiked samples revealed immunomodulatory activity of both native and denatured uSPG. Protein array showed that native uSPG activates certain cytokines and chemokines significantly stronger than denatured uSPG.

Citovat

SMIRNOU, Dzianis; Petr KNOTEK; Kristina NEŠPOROVÁ; Daniela SMEJKALOVA; Vojtech PAVLIK; Lukas FRANKE a Vladimir VELEBNY. Ultrasound-assisted production of highly-purified beta-glucan schizophyllan and characterization of its immune properties. Process Biochemistry. Oxford: ELSEVIER SCI LTD, 2017, roč. 58, JUL, s. 313-319. ISSN 1359-5113. Dostupné z: https://doi.org/10.1016/j.procbio.2017.04.016.

@article{1597139,
   author = {Smirnou, Dzianis and Knotek, Petr and Nešporová, Kristina and Smejkalova, Daniela and Pavlik, Vojtech and Franke, Lukas and Velebny, Vladimir},
   article_location = {Oxford},
   article_number = {JUL},
   doi = {https://doi.org/10.1016/j.procbio.2017.04.016},
   keywords = {Schizophyllan ultrasound; Downstream; Proteins; Endotoxins; Immune effect},
   language = {eng},
   issn = {1359-5113},
   journal = {Process Biochemistry},
   title = {Ultrasound-assisted production of highly-purified beta-glucan schizophyllan and characterization of its immune properties},
   url = {https://www.sciencedirect.com/science/article/pii/S1359511316310613},
   volume = {58},
   year = {2017}
}

TY  - JOUR
ID  - 1597139
AU  - Smirnou, Dzianis - Knotek, Petr - Nešporová, Kristina - Smejkalova, Daniela - Pavlik, Vojtech - Franke, Lukas - Velebny, Vladimir
PY  - 2017
TI  - Ultrasound-assisted production of highly-purified beta-glucan schizophyllan and characterization of its immune properties
JF  - Process Biochemistry
VL  - 58
IS  - JUL
SP  - 313-319
EP  - 313-319
PB  - ELSEVIER SCI LTD
SN  - 13595113
KW  - Schizophyllan ultrasound
KW  - Downstream
KW  - Proteins
KW  - Endotoxins
KW  - Immune effect
UR  - https://www.sciencedirect.com/science/article/pii/S1359511316310613
L2  - https://www.sciencedirect.com/science/article/pii/S1359511316310613
N2  - In this work, we suggest procedure for manufacturing of highly pure schizophyllan (SPG) cleaved into shorter fragments by ultrasonication. The ultrasonicated SPG (uSPG) was prepared from Schizophyllum commune submerged cultivation in bioreactor, culture broth ultrasonication with 100 Ws/mL energy input, followed by depth filtration and ultrafiltration. The uSPG contained less than 0.3%w/w protein and 0.078 IU/mg endotoxin. Chemical composition and structure of the uSPG were analyzed by GC and NMR, and conformation of the molecules was documented by AFM. Apyrogenicity of uSPG was proved by MAT test. Immunomodulatory properties of native and denatured uSPG were studied in vitro with human whole blood. The LPS-spiked samples revealed immunomodulatory activity of both native and denatured uSPG. Protein array showed that native uSPG activates certain cytokines and chemokines significantly stronger than denatured uSPG.
ER  -

SMIRNOU, Dzianis; Petr KNOTEK; Kristina NEŠPOROVÁ; Daniela SMEJKALOVA; Vojtech PAVLIK; Lukas FRANKE a Vladimir VELEBNY. Ultrasound-assisted production of highly-purified beta-glucan schizophyllan and characterization of its immune properties. \textit{Process Biochemistry}. Oxford: ELSEVIER SCI LTD, 2017, roč.~58, JUL, s.~313-319. ISSN~1359-5113. Dostupné z: https://doi.org/10.1016/j.procbio.2017.04.016.

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