Osteogenic Potential of Caspases Related to Endochondral
Ossification

J 2018

Osteogenic Potential of Caspases Related to Endochondral Ossification

JANEČKOVÁ, Eva; Petra BILIKOVA a Eva MATALOVA

Základní údaje

Originální název

Osteogenic Potential of Caspases Related to Endochondral Ossification

Autoři

JANEČKOVÁ, Eva; Petra BILIKOVA a Eva MATALOVA

Vydání

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, LONDON, SAGE PUBLICATIONS LTD, 2018, 0022-1554

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.370

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00111965

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

caspases; endochondral ossification; growth plate; immunohistochemistry; micromass cultures; PCR Array analysis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 5. 2020 10:32, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Caspases have functions particularly in apoptosis and inflammation. Increasing evidence indicates novel roles of these proteases in cell differentiation, including those involved in osteogenesis. This investigation provides a complex screening of osteogenic markers affected by pan caspase inhibition in micromass cultures derived from mouse forelimbs. PCR Array analysis showed significant alterations in expression of 49 osteogenic genes after 7 days of inhibition. The largest change was a decrease in CD36 expression, which was confirmed at organ level by caspase inhibition in cultured mouse ulnae followed by CD36 immunohistochemical analysis. So far, available data point to osteogenic potential of pro-apoptotic caspases. Therefore, the expression of pro-apoptotic caspases (-3, -6, -7, -8, -9) within the growth plate of mouse forelimbs at the stage where the individual zones are clearly apparent was studied. Caspase-9 was reported in the growth plate for the first time as well as caspase-6 and -7 in the resting zone, caspase-7 in the proliferation, and caspase-6 and -8 in the ossification zone. For all caspases, there was a gradient increase in activation toward the ossification zone. The distribution of staining varied significantly from that of apoptotic cells, and thus, the results further support non-apoptotic participation of caspases in osteogenesis.

Citovat

JANEČKOVÁ, Eva; Petra BILIKOVA a Eva MATALOVA. Osteogenic Potential of Caspases Related to Endochondral Ossification. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. LONDON: SAGE PUBLICATIONS LTD, 2018, roč. 66, č. 1, s. 47-58. ISSN 0022-1554. Dostupné z: https://doi.org/10.1369/0022155417739283.

@article{1597596,
   author = {Janečková, Eva and Bilikova, Petra and Matalova, Eva},
   article_location = {LONDON},
   article_number = {1},
   doi = {https://doi.org/10.1369/0022155417739283},
   keywords = {caspases; endochondral ossification; growth plate; immunohistochemistry; micromass cultures; PCR Array analysis},
   language = {eng},
   issn = {0022-1554},
   journal = {JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY},
   title = {Osteogenic Potential of Caspases Related to Endochondral Ossification},
   url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761947/pdf/10.1369_0022155417739283.pdf},
   volume = {66},
   year = {2018}
}

TY  - JOUR
ID  - 1597596
AU  - Janečková, Eva - Bilikova, Petra - Matalova, Eva
PY  - 2018
TI  - Osteogenic Potential of Caspases Related to Endochondral Ossification
JF  - JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
VL  - 66
IS  - 1
SP  - 47-58
EP  - 47-58
PB  - SAGE PUBLICATIONS LTD
SN  - 00221554
KW  - caspases
KW  - endochondral ossification
KW  - growth plate
KW  - immunohistochemistry
KW  - micromass cultures
KW  - PCR Array analysis
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761947/pdf/10.1369_0022155417739283.pdf
L2  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5761947/pdf/10.1369_0022155417739283.pdf
N2  - Caspases have functions particularly in apoptosis and inflammation. Increasing evidence indicates novel roles of these proteases in cell differentiation, including those involved in osteogenesis. This investigation provides a complex screening of osteogenic markers affected by pan caspase inhibition in micromass cultures derived from mouse forelimbs. PCR Array analysis showed significant alterations in expression of 49 osteogenic genes after 7 days of inhibition. The largest change was a decrease in CD36 expression, which was confirmed at organ level by caspase inhibition in cultured mouse ulnae followed by CD36 immunohistochemical analysis. So far, available data point to osteogenic potential of pro-apoptotic caspases. Therefore, the expression of pro-apoptotic caspases (-3, -6, -7, -8, -9) within the growth plate of mouse forelimbs at the stage where the individual zones are clearly apparent was studied. Caspase-9 was reported in the growth plate for the first time as well as caspase-6 and -7 in the resting zone, caspase-7 in the proliferation, and caspase-6 and -8 in the ossification zone. For all caspases, there was a gradient increase in activation toward the ossification zone. The distribution of staining varied significantly from that of apoptotic cells, and thus, the results further support non-apoptotic participation of caspases in osteogenesis.
ER  -

JANEČKOVÁ, Eva; Petra BILIKOVA a Eva MATALOVA. Osteogenic Potential of Caspases Related to Endochondral Ossification. \textit{JOURNAL OF HISTOCHEMISTRY \&{}amp; CYTOCHEMISTRY}. LONDON: SAGE PUBLICATIONS LTD, 2018, roč.~66, č.~1, s.~47-58. ISSN~0022-1554. Dostupné z: https://doi.org/10.1369/0022155417739283.

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