Capillary electrophoresis, a method for the determination of
nucleic acid ligands covalently attached to quantum dots
representing a donor of Forster resonance energy transfer

J 2018

Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer

DATINSKÁ, Vladimíra; Karel KLEPARNIK; Barbora BELSANOVA; Marek MINARIK; Frantisek FORET et al.

Základní údaje

Originální název

Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer

Autoři

DATINSKÁ, Vladimíra; Karel KLEPARNIK; Barbora BELSANOVA; Marek MINARIK a Frantisek FORET

Vydání

Journal of Separation Science, Weinheim, WILEY-V C H VERLAG GMBH, 2018, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 2.516

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00112184

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

capillary electrophoresis; Forster resonance energy transfer; nucleic acids; quantum dots; sensors

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 1. 2020 11:24, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The synthesis and determination of the structure of a Forster resonance energy transfer probe intended for the detection of specific nucleic acid sequences are described here. The probe is based on the hybridization of oligonucleotide modified quantum dots with a fluorescently labeled nucleic acid sample resulting in changes of the fluorescence emission due to the energy transfer effect. The stoichiometry distribution of oligonucleotides conjugated to quantum dots was determined by capillary electrophoresis separation. The results indicate that one to four molecules of oligonucleotide are conjugated to the surface of a single nanoparticle. This conclusion is confirmed by the course of the dependence of Forster resonance energy transfer efficiency on the concentration of fluorescently labeled complementary single-stranded nucleic acid, showing saturation. While the energy transfer efficiency of the probe hybridized with complementary nucleic acid strands was 30%, negligible efficiency was observed with a noncomplementary strand.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

DATINSKÁ, Vladimíra; Karel KLEPARNIK; Barbora BELSANOVA; Marek MINARIK a Frantisek FORET. Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer. Journal of Separation Science. Weinheim: WILEY-V C H VERLAG GMBH, 2018, roč. 41, č. 14, s. 1-9. ISSN 1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201800248.

@article{1601051,
   author = {Datinská, Vladimíra and Kleparnik, Karel and Belsanova, Barbora and Minarik, Marek and Foret, Frantisek},
   article_location = {Weinheim},
   article_number = {14},
   doi = {https://doi.org/10.1002/jssc.201800248},
   keywords = {capillary electrophoresis; Forster resonance energy transfer; nucleic acids; quantum dots; sensors},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer},
   url = {https://onlinelibrary.wiley.com/doi/pdf/10.1002/jssc.201800248},
   volume = {41},
   year = {2018}
}

TY  - JOUR
ID  - 1601051
AU  - Datinská, Vladimíra - Kleparnik, Karel - Belsanova, Barbora - Minarik, Marek - Foret, Frantisek
PY  - 2018
TI  - Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer
JF  - Journal of Separation Science
VL  - 41
IS  - 14
SP  - 1-9
EP  - 1-9
PB  - WILEY-V C H VERLAG GMBH
SN  - 16159306
KW  - capillary electrophoresis
KW  - Forster resonance energy transfer
KW  - nucleic acids
KW  - quantum dots
KW  - sensors
UR  - https://onlinelibrary.wiley.com/doi/pdf/10.1002/jssc.201800248
L2  - https://onlinelibrary.wiley.com/doi/pdf/10.1002/jssc.201800248
N2  - The synthesis and determination of the structure of a Forster resonance energy transfer probe intended for the detection of specific nucleic acid sequences are described here. The probe is based on the hybridization of oligonucleotide modified quantum dots with a fluorescently labeled nucleic acid sample resulting in changes of the fluorescence emission due to the energy transfer effect. The stoichiometry distribution of oligonucleotides conjugated to quantum dots was determined by capillary electrophoresis separation. The results indicate that one to four molecules of oligonucleotide are conjugated to the surface of a single nanoparticle. This conclusion is confirmed by the course of the dependence of Forster resonance energy transfer efficiency on the concentration of fluorescently labeled complementary single-stranded nucleic acid, showing saturation. While the energy transfer efficiency of the probe hybridized with complementary nucleic acid strands was 30%, negligible efficiency was observed with a noncomplementary strand.
ER  -

DATINSKÁ, Vladimíra; Karel KLEPARNIK; Barbora BELSANOVA; Marek MINARIK a Frantisek FORET. Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer. \textit{Journal of Separation Science}. Weinheim: WILEY-V C H VERLAG GMBH, 2018, roč.~41, č.~14, s.~1-9. ISSN~1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201800248.

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