Production and characterization of galactose-specific lectin
PluLec from plant pathogen Photorhabdus luminescens

a 2019

Production and characterization of galactose-specific lectin PluLec from plant pathogen Photorhabdus luminescens

KORSÁK, Marek, Josef HOUSER, Eva FUJDIAROVÁ, Marie POKORNÁ, Jan KOMÁREK et. al.

Základní údaje

Originální název

Production and characterization of galactose-specific lectin PluLec from plant pathogen Photorhabdus luminescens

Autoři

KORSÁK, Marek (703 Slovensko, domácí), Josef HOUSER (203 Česká republika, domácí), Eva FUJDIAROVÁ (203 Česká republika, domácí), Marie POKORNÁ (203 Česká republika, domácí), Jan KOMÁREK (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí)

Vydání

XX. setkání biochemiků a molekulárních biologů, 2019. 2019

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/19:00112191

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-9420-8

Klíčová slova česky

Photorhabdus luminescens;lektin

Klíčová slova anglicky

Photorhabdus luminescens;lectin

Změněno: 23. 1. 2020 01:33, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

V originále

Lectins are a group of proteins of non-immune origin that can reversibly and specifically interact with carbohydrates. They are found in all known living organisms and are involved in recognition events in various physiological and pathological processes like intercellular communication, adhesion, migration and host-pathogen interactions. Lectins are commonly used for characterization of carbohydrate structures, for purification of glycoproteins and to specifically label the cell surface structures. They are also used for cell separation and typing. Photorhabdus luminescens is a naturally bioluminescent Gram-negative bacterium which symbiotically lives in Heterorhabditidae nematodes and is strongly pathogenic to insect larvae. PluLec, a putative lectin from Photorhabdus luminescens that we discovered, is a homologue of PA-IL lectin, which is D galactose specific, Ca2+ dependent, cytotoxic lectin from opportunistic pathogen Pseudomonas aeruginosa, involved in facilitating infection in patients with compromised immunity. This research is focused on cloning, production and structural-functional characterization of recombinant protein PluLec using various methods, namely dynamic light scattering, differential scanning fluorimetry, isothermal titration calorimetry, hemagglutination, glycan array, surface plasmon resonance, analytical ultracentrifugation and protein X-ray crystallography. Detailed knowledge of the structure and function of PluLec can help to understand the mechanism of P. luminescens infection.

Návaznosti

MUNI/A/1575/2018, interní kód MU

Název: Podpora biochemického výzkumu v roce 2019

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2019, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KORSÁK, Marek, Josef HOUSER, Eva FUJDIAROVÁ, Marie POKORNÁ, Jan KOMÁREK a Michaela WIMMEROVÁ. Production and characterization of galactose-specific lectin PluLec from plant pathogen Photorhabdus luminescens. In XX. setkání biochemiků a molekulárních biologů, 2019. 2019. ISBN 978-80-210-9420-8.

@proceedings{1601178,
   author = {Korsák, Marek and Houser, Josef and Fujdiarová, Eva and Pokorná, Marie and Komárek, Jan and Wimmerová, Michaela},
   booktitle = {XX. setkání biochemiků a molekulárních biologů, 2019.},
   keywords = {Photorhabdus luminescens;lectin},
   language = {eng},
   isbn = {978-80-210-9420-8},
   title = {Production and characterization of galactose-specific lectin PluLec from plant pathogen Photorhabdus luminescens},
   year = {2019}
}

TY  - CONF
ID  - 1601178
AU  - Korsák, Marek - Houser, Josef - Fujdiarová, Eva - Pokorná, Marie - Komárek, Jan - Wimmerová, Michaela
PY  - 2019
TI  - Production and characterization of galactose-specific lectin PluLec from plant pathogen Photorhabdus luminescens
SN  - 9788021094208
KW  - Photorhabdus luminescens;lectin
N2  - Lectins are a group of proteins of non-immune origin that can reversibly and specifically interact with carbohydrates. They are found in all known living organisms and are involved in recognition events in various physiological and pathological processes like intercellular communication, adhesion, migration and host-pathogen interactions. Lectins are commonly used for characterization of carbohydrate structures, for purification of glycoproteins and to specifically label the cell surface structures. They are also used for cell separation and typing. Photorhabdus luminescens is a naturally bioluminescent Gram-negative bacterium which symbiotically lives in Heterorhabditidae nematodes and is strongly pathogenic to insect larvae. PluLec, a putative lectin from Photorhabdus luminescens that we discovered, is a homologue of PA-IL lectin, which is D galactose specific, Ca2+ dependent, cytotoxic lectin from opportunistic pathogen Pseudomonas aeruginosa, involved in facilitating infection in patients with compromised immunity. This research is focused on cloning, production and structural-functional characterization of recombinant protein PluLec using various methods, namely dynamic light scattering, differential scanning fluorimetry, isothermal titration calorimetry, hemagglutination, glycan array, surface plasmon resonance, analytical ultracentrifugation and protein X-ray crystallography. Detailed knowledge of the structure and function of PluLec can help to understand the mechanism of P. luminescens infection.
ER  -

KORSÁK, Marek, Josef HOUSER, Eva FUJDIAROVÁ, Marie POKORNÁ, Jan KOMÁREK a Michaela WIMMEROVÁ. Production and characterization of galactose-specific lectin PluLec from plant pathogen Photorhabdus luminescens. In \textit{XX. setkání biochemiků a molekulárních biologů, 2019.}. 2019. ISBN~978-80-210-9420-8.

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