Cellulose fibrils formation and organisation of cytoskeleton
during encystment are essential for Acanthamoeba cyst wall
architecture

J 2019

Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture

GARAJOVÁ, Mária; Martin MRVA; Naděžda VAŠKOVICOVÁ; Michal MARTINKA; Janka MELICHEROVÁ et al.

Základní údaje

Originální název

Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture

Autoři

GARAJOVÁ, Mária; Martin MRVA; Naděžda VAŠKOVICOVÁ; Michal MARTINKA; Janka MELICHEROVÁ a Andrea BARDŮNEK VALIGUROVÁ

Vydání

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2019, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10613 Zoology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.998

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00108007

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

Acanthamoeba;cysta;endocysta;ektocysta;celulóza;mikroskpie

Klíčová slova anglicky

Acanthamoeba;cyst;endocyst;ectocyst;cellulose;microscopy

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 3. 2020 14:54, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Acanthamoebae success as human pathogens is largely due to the highly resistant cysts which represent a crucial problem in treatment of Acanthamoeba infections. Hence, the study of cyst wall composition and encystment play an important role in finding new therapeutic strategies. For the first time, we detected high activity of cytoskeletal elements-microtubular networks and filamentous actin, in late phases of encystment. Cellulose fibrils-the main components of endocyst were demonstrated in inter-cystic space, and finally in the ectocyst, hereby proving the presence of cellulose in both layers of the cyst wall. We detected clustering of intramembranous particles (IMPs) and their density alterations in cytoplasmic membrane during encystment. We propose a hypothesis that in the phase of endocyst formation, the IMP clusters represent cellulose microfibril terminal complexes involved in cellulose synthesis that after cyst wall completion are reduced. Cyst wall impermeability, due largely to a complex polysaccharide (glycans, mainly cellulose) has been shown to be responsible for Acanthamoeba biocide resistance and cellulose biosynthesis pathway is suggested to be a potential target in treatment of Acanthamoeba infections. Disruption of this pathway would affect the synthesis of cyst wall and reduce considerably the resistance to chemotherapeutic agents.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

7AMB14SK008, projekt VaV

Název: Cytologické změny izolátů akantaméb po aplikaci potenciálně amebicidních sloučenin (Akronym: CYTAPAC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Cytologické změny izolátů akantaméb po aplikaci potenciálně amebicidních sloučenin

Citovat

GARAJOVÁ, Mária; Martin MRVA; Naděžda VAŠKOVICOVÁ; Michal MARTINKA; Janka MELICHEROVÁ a Andrea BARDŮNEK VALIGUROVÁ. Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2019, roč. 9, MAR, s. 1-21. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-019-41084-6.

@article{1603158,
   author = {Garajová, Mária and Mrva, Martin and Vaškovicová, Naděžda and Martinka, Michal and Melicherová, Janka and Bardůnek Valigurová, Andrea},
   article_location = {LONDON},
   article_number = {MAR},
   doi = {https://doi.org/10.1038/s41598-019-41084-6},
   keywords = {Acanthamoeba;cyst;endocyst;ectocyst;cellulose;microscopy},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture},
   url = {https://www.nature.com/articles/s41598-019-41084-6},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1603158
AU  - Garajová, Mária - Mrva, Martin - Vaškovicová, Naděžda - Martinka, Michal - Melicherová, Janka - Bardůnek Valigurová, Andrea
PY  - 2019
TI  - Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture
JF  - Scientific reports
VL  - 9
IS  - MAR
SP  - 1-21
EP  - 1-21
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - Acanthamoeba;cyst;endocyst;ectocyst;cellulose;microscopy
UR  - https://www.nature.com/articles/s41598-019-41084-6
L2  - https://www.nature.com/articles/s41598-019-41084-6
N2  - Acanthamoebae success as human pathogens is largely due to the highly resistant cysts which represent a crucial problem in treatment of Acanthamoeba infections. Hence, the study of cyst wall composition and encystment play an important role in finding new therapeutic strategies. For the first time, we detected high activity of cytoskeletal elements-microtubular networks and filamentous actin, in late phases of encystment. Cellulose fibrils-the main components of endocyst were demonstrated in inter-cystic space, and finally in the ectocyst, hereby proving the presence of cellulose in both layers of the cyst wall. We detected clustering of intramembranous particles (IMPs) and their density alterations in cytoplasmic membrane during encystment. We propose a hypothesis that in the phase of endocyst formation, the IMP clusters represent cellulose microfibril terminal complexes involved in cellulose synthesis that after cyst wall completion are reduced. Cyst wall impermeability, due largely to a complex polysaccharide (glycans, mainly cellulose) has been shown to be responsible for Acanthamoeba biocide resistance and cellulose biosynthesis pathway is suggested to be a potential target in treatment of Acanthamoeba infections. Disruption of this pathway would affect the synthesis of cyst wall and reduce considerably the resistance to chemotherapeutic agents.
ER  -

GARAJOVÁ, Mária; Martin MRVA; Naděžda VAŠKOVICOVÁ; Michal MARTINKA; Janka MELICHEROVÁ a Andrea BARDŮNEK VALIGUROVÁ. Cellulose fibrils formation and organisation of cytoskeleton during encystment are essential for Acanthamoeba cyst wall architecture. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2019, roč.~9, MAR, s.~1-21. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-019-41084-6.

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