Další formáty:
BibTeX
LaTeX
RIS
@article{1603717,
author = {Ondracek, Karel and Bandouchova, Hana and Hilscherová, Klára and Kovacova, Veronika and Linhart, Petr and Miksikova, Miroslava and Mlčáková, Veronika and Osickova, Jitka and Pohanka, Miroslav and Skochova, Hana and Pikula, Jiri},
article_location = {Munsbach},
article_number = {2015},
keywords = {FETAX; acetylcholinesterase inhibitor; anticoagulant; cyanotoxin; combination toxicity; oxidative stress; caspase},
language = {eng},
issn = {0172-780X},
journal = {Neuroendocrinology Letters},
title = {Mixture toxicity of microcystin-LR, paraoxon and bromadiolone in Xenopus laevis embryos},
url = {http://www.nel.edu/userfiles/articlesnew/NEL360915A13.pdf},
volume = {36},
year = {2015}
}
TY - JOUR
ID - 1603717
AU - Ondracek, Karel - Bandouchova, Hana - Hilscherová, Klára - Kovacova, Veronika - Linhart, Petr - Miksikova, Miroslava - Mlčáková, Veronika - Osickova, Jitka - Pohanka, Miroslav - Skochova, Hana - Pikula, Jiri
PY - 2015
TI - Mixture toxicity of microcystin-LR, paraoxon and bromadiolone in Xenopus laevis embryos
JF - Neuroendocrinology Letters
VL - 36
IS - 2015
SP - 114-119
EP - 114-119
PB - Maghira & Maas publications
SN - 0172780X
KW - FETAX
KW - acetylcholinesterase inhibitor
KW - anticoagulant
KW - cyanotoxin
KW - combination toxicity
KW - oxidative stress
KW - caspase
UR - http://www.nel.edu/userfiles/articlesnew/NEL360915A13.pdf
L2 - http://www.nel.edu/userfiles/articlesnew/NEL360915A13.pdf
N2 - OBJECTIVES: Apart from infections and habitat loss, environmental pollution is another major factor of global decline of amphibians. Using the model of Xenopus laevis embryos, we test the hypothesis that combined exposure of amphibians to natural toxins and anthropogenic pollutants induces more pronounced adverse effects than single exposures. METHODS: Experimental procedures adhered to Frog Embryo Teratogenesis Assay - Xenopus standards (FETAX). Exposure groups included controls, solvent (dimethyl sulfoxide) controls, and embryos exposed for 96 h to single, double and triple action of paraoxon (P), bromadiolone (B), and microcystin-LR (M), added to the FETAX medium at a dose of 300, 350, and 500 mu g.L-1, respectively. Studied responses of X. laevis embryos included mortality and malformations, head-to-tail length, total antioxidant capacity, lipid peroxidation, and caspase-3 activity. RESULTS: The triple combination induced the highest mortality. Malformations in embryos significantly prevailed only in B-, and B+P-exposure groups. Apart from the single exposure to B, the tested substances and their combinations inhibited the embryonic growth. Triple exposure had the most pronounced effect both on the growth inhibition and total antioxidant capacity. Lipid peroxidation was increased after B+ M exposure, while single and combined exposures to B and P had an opposite effect. CONCLUSIONS: This study helps to understand adverse effects of environmental pollution by natural toxins and agrochemicals in amphibians. The results allow for risk assessment of environmental pollution and findings of low concentrations of contaminants in aquatic environments. Further research to address issues such as mixture toxicity to metamorphosing and adult amphibians is necessary.
ER -
ONDRACEK, Karel, Hana BANDOUCHOVA, Klára HILSCHEROVÁ, Veronika KOVACOVA, Petr LINHART, Miroslava MIKSIKOVA, Veronika MLČÁKOVÁ, Jitka OSICKOVA, Miroslav POHANKA, Hana SKOCHOVA a Jiri PIKULA. Mixture toxicity of microcystin-LR, paraoxon and bromadiolone in Xenopus laevis embryos. \textit{Neuroendocrinology Letters}. Munsbach: Maghira \&{} Maas publications, 2015, roč.~36, č.~2015, s.~114-119. ISSN~0172-780X.
|
