Preparation and evaluation of carriers for detection of
cholinesterase inhibitors

J 2015

Preparation and evaluation of carriers for detection of cholinesterase inhibitors

VETCHÝ, David, Vladimír PITSCHMANN, Marta VETCHÁ, Tomáš KAŠPAROVSKÝ, Lukáš MATĚJOVSKÝ et. al.

Základní údaje

Originální název

Preparation and evaluation of carriers for detection of cholinesterase inhibitors

Autoři

VETCHÝ, David, Vladimír PITSCHMANN, Marta VETCHÁ, Tomáš KAŠPAROVSKÝ (203 Česká republika, domácí) a Lukáš MATĚJOVSKÝ

Vydání

Neuroendocrinology Letters, Munsbach, Maghira & Maas publications, 2015, 0172-780X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30103 Neurosciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 0.946

Kód RIV

RIV/00216224:14310/15:00112362

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000369404400014

Klíčová slova anglicky

detection tubes; cholinesterase inhibitors; pellets; intraparticular porosity; total tapped porosity; sphericity; pellet regularity; acetylcholinesterase activity

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 1. 2020 09:37, Mgr. Marie Šípková, DiS.

Anotace

V originále

OBJECTIVES: The aim of the study was to use methods of pharmaceutical technology, and prepare carriers in the form of pellets suitable as a filling of detection tubes for enzymatic detection of cholinesterase inhibitors. The enzymatic detection was based on enzymatic hydrolysis of acetylthiocholine iodide and the subsequent colour reaction of its hydrolysis product with Ellman's reagent. The suitable carriers should be in the form of white, regular and sufficiently mechanically resistant particles of about 1 mm allowing it to capture the enzyme during the impregnation process and ensuring its high activity for enzymatic detection. METHODS: Carriers consisting of microcrystalline cellulose, lactose, povidone, and sodium carboxymethyl cellulose were prepared using extrusion-spheronization method under three different drying conditions in either a hot air oven or a microwave oven. Subsequently, the carriers were impregnated with acetylcholinesterase and their size, shape, mechanical resistance, bulk, tapped and pycnometric density, Hausner ratio, intraparticular and total tapped porosity, and activity were measured and recorded. RESULTS: In this procedure, carriers with different physical parameters and different acetylcholinesterase activity were evaluated. It was found that higher acetylcholinesterase activity was associated not only with a higher intraparticular porosity but also with more regular particles characterized by high sphericity and low total tapped porosity. CONCLUSION: This unique finding is important for the preparation of detection tubes based on enzymatic detection which is still irreplaceable especially in the field of detection and analysis of super-toxic cholinesterase inhibitors.

Citovat

VETCHÝ, David, Vladimír PITSCHMANN, Marta VETCHÁ, Tomáš KAŠPAROVSKÝ a Lukáš MATĚJOVSKÝ. Preparation and evaluation of carriers for detection of cholinesterase inhibitors. Neuroendocrinology Letters. Munsbach: Maghira & Maas publications, 2015, roč. 36, č. 2015, s. 95-99. ISSN 0172-780X.

@article{1603720,
   author = {Vetchý, David and Pitschmann, Vladimír and Vetchá, Marta and Kašparovský, Tomáš and Matějovský, Lukáš},
   article_location = {Munsbach},
   article_number = {2015},
   keywords = {detection tubes; cholinesterase inhibitors; pellets; intraparticular porosity; total tapped porosity; sphericity; pellet regularity; acetylcholinesterase activity},
   language = {eng},
   issn = {0172-780X},
   journal = {Neuroendocrinology Letters},
   title = {Preparation and evaluation of carriers for detection of cholinesterase inhibitors},
   url = {http://www.nel.edu/userfiles/articlesnew/NEL360915A22.pdf},
   volume = {36},
   year = {2015}
}

TY  - JOUR
ID  - 1603720
AU  - Vetchý, David - Pitschmann, Vladimír - Vetchá, Marta - Kašparovský, Tomáš - Matějovský, Lukáš
PY  - 2015
TI  - Preparation and evaluation of carriers for detection of cholinesterase inhibitors
JF  - Neuroendocrinology Letters
VL  - 36
IS  - 2015
SP  - 95-99
EP  - 95-99
PB  - Maghira & Maas publications
SN  - 0172780X
KW  - detection tubes
KW  - cholinesterase inhibitors
KW  - pellets
KW  - intraparticular porosity
KW  - total tapped porosity
KW  - sphericity
KW  - pellet regularity
KW  - acetylcholinesterase activity
UR  - http://www.nel.edu/userfiles/articlesnew/NEL360915A22.pdf
L2  - http://www.nel.edu/userfiles/articlesnew/NEL360915A22.pdf
N2  - OBJECTIVES: The aim of the study was to use methods of pharmaceutical technology, and prepare carriers in the form of pellets suitable as a filling of detection tubes for enzymatic detection of cholinesterase inhibitors. The enzymatic detection was based on enzymatic hydrolysis of acetylthiocholine iodide and the subsequent colour reaction of its hydrolysis product with Ellman's reagent. The suitable carriers should be in the form of white, regular and sufficiently mechanically resistant particles of about 1 mm allowing it to capture the enzyme during the impregnation process and ensuring its high activity for enzymatic detection. METHODS: Carriers consisting of microcrystalline cellulose, lactose, povidone, and sodium carboxymethyl cellulose were prepared using extrusion-spheronization method under three different drying conditions in either a hot air oven or a microwave oven. Subsequently, the carriers were impregnated with acetylcholinesterase and their size, shape, mechanical resistance, bulk, tapped and pycnometric density, Hausner ratio, intraparticular and total tapped porosity, and activity were measured and recorded. RESULTS: In this procedure, carriers with different physical parameters and different acetylcholinesterase activity were evaluated. It was found that higher acetylcholinesterase activity was associated not only with a higher intraparticular porosity but also with more regular particles characterized by high sphericity and low total tapped porosity. CONCLUSION: This unique finding is important for the preparation of detection tubes based on enzymatic detection which is still irreplaceable especially in the field of detection and analysis of super-toxic cholinesterase inhibitors.
ER  -

VETCHÝ, David, Vladimír PITSCHMANN, Marta VETCHÁ, Tomáš KAŠPAROVSKÝ a Lukáš MATĚJOVSKÝ. Preparation and evaluation of carriers for detection of cholinesterase inhibitors. \textit{Neuroendocrinology Letters}. Munsbach: Maghira \&{} Maas publications, 2015, roč.~36, č.~2015, s.~95-99. ISSN~0172-780X.

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