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@article{1604540,
author = {Lo Re, Oriana and Maugeri, Andrea and Hrušková, Jana and Jakubík, Juraj and Kučera, Jan and Dobrovolná, Julie and Oben, Jude A. and Kubala, Lukáš and Dvořáková, Adéla and Číž, Milan and Vinciguerra, Manlio},
article_location = {LONDON},
article_number = {1},
doi = {http://dx.doi.org/10.1186/s13148-019-0797-8},
keywords = {Liquid biopsy; Nucleosome; Epigenetics; Metabolic health; Cardiovascular disease},
language = {eng},
issn = {1868-7075},
journal = {CLINICAL EPIGENETICS},
title = {Obesity-induced nucleosome release predicts poor cardio-metabolic health},
url = {http://dx.doi.org/10.1186/s13148-019-0797-8},
volume = {12},
year = {2019}
}
TY - JOUR
ID - 1604540
AU - Lo Re, Oriana - Maugeri, Andrea - Hrušková, Jana - Jakubík, Juraj - Kučera, Jan - Dobrovolná, Julie - Oben, Jude A. - Kubala, Lukáš - Dvořáková, Adéla - Číž, Milan - Vinciguerra, Manlio
PY - 2019
TI - Obesity-induced nucleosome release predicts poor cardio-metabolic health
JF - CLINICAL EPIGENETICS
VL - 12
IS - 1
SP - 1-14
EP - 1-14
PB - BMC
SN - 18687075
KW - Liquid biopsy
KW - Nucleosome
KW - Epigenetics
KW - Metabolic health
KW - Cardiovascular disease
UR - http://dx.doi.org/10.1186/s13148-019-0797-8
L2 - http://dx.doi.org/10.1186/s13148-019-0797-8
N2 - Objective While circulating nucleosome levels are high in obese mouse models, it is unknown where these nucleosomes originate from and whether they are a marker of cardio-metabolic health in humans. Here, we aimed to determine whether an association exists between circulating nucleosomes and the risk of developing obesity, metabolic syndrome (MetS) and/or a dysfunctional cardiovascular performance. Methods We randomly selected 120 participants of the Kardiovize Brno 2030 study across three BMI strata: BMI 18-25, 25-30, and > 30. We assessed the association between circulating nucleosome levels and the risk of obesity, MetS, and poor cardiovascular health. We then cultured human neutrophils, adipocytes, and hepatoma cells to study nucleosome origins in a fat-rich environment. Results Circulating nucleosome levels positively correlated with BMI (R = 0.602, p < 0.05), fatty liver index (R = 0.622, p < 0.05), left ventricular mass (R = 0.457, p < 0.05), and associated with MetS (p < 0.001) and poor cardiovascular health (p < 0.001). Incubating neutrophils with 1-10 mu M free fatty acids triggered nucleosome production without concomitant cell death. Nucleosomes were not produced during pre-adipocyte differentiation or upon incubation of hepatic cells with palmitic acid. Conclusions Neutrophils are a bona fide source of circulating nucleosomes in an obesogenic environment and in overweight/obese patients. High nucleosome levels are associated with MetS and cardiovascular performance, and might represent novel candidate biomarkers for cardio-metabolic health.
ER -
LO RE, Oriana, Andrea MAUGERI, Jana HRUŠKOVÁ, Juraj JAKUBÍK, Jan KUČERA, Julie DOBROVOLNÁ, Jude A. OBEN, Lukáš KUBALA, Adéla DVOŘÁKOVÁ, Milan ČÍŽ a Manlio VINCIGUERRA. Obesity-induced nucleosome release predicts poor cardio-metabolic health. \textit{CLINICAL EPIGENETICS}. LONDON: BMC, roč.~12, č.~1, s.~1-14. ISSN~1868-7075. doi:10.1186/s13148-019-0797-8. 2019.
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