The Unique Mechanisms of Cellular Proliferation, Migration and
Apoptosis are Regulated through Oocyte Maturational
DevelopmentA Complete Transcriptomic and Histochemical Study

J 2019

The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study

CHERMULA, Blazej; Maciej BRAZERT; Michal JEŠETA; Katarzyna OZEGOWSKA; Patrycja SUJKA-KORDOWSKA et al.

Základní údaje

Originální název

The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study

Autoři

Vydání

International Journal of Molecular Sciences, Basel, Switzerland, MDPI AG, 2019, 1422-0067

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/19:00112512

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

pig; oocytes; microarray; cellular competence

Štítky

14110411, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 1. 2020 13:17, Mgr. Tereza Miškechová

Anotace

V originále

The growth and development of oocyte affect the functional activities of the surrounding somatic cells. These cells are regulated by various types of hormones, proteins, metabolites, and regulatory molecules through gap communication, ultimately leading to the development and maturation of oocytes. The close association between somatic cells and oocytes, which together form the cumulus-oocyte complexes (COCs), and their bi-directional communication are crucial for the acquisition of developmental competences by the oocyte. In this study, oocytes were extracted from the ovaries obtained from crossbred landrace gilts and subjected to in vitro maturation. RNA isolated from those oocytes was used for the subsequent microarray analysis. The data obtained shows, for the first time, variable levels of gene expression (fold changes higher than |2| and adjusted p-value < 0.05) belonging to four ontological groups: regulation of cell proliferation (GO:0042127), regulation of cell migration (GO:0030334), and regulation of programmed cell death (GO:0043067) that can be used together as proliferation, migration or apoptosis markers. We have identified several genes of porcine oocytes (ID2, VEGFA, BTG2, ESR1, CCND2, EDNRA, ANGPTL4, TGFBR3, GJA1, LAMA2, KIT, TPM1, VCP, GRID2, MEF2C, RPS3A, PLD1, BTG3, CD47, MITF), whose expression after in vitro maturation (IVM) is downregulated with different degrees. Our results may be helpful in further elucidating the molecular basis and functional significance of a number of gene markers associated with the processes of migration, proliferation and angiogenesis occurring in COCs.

Citovat

CHERMULA, Blazej; Maciej BRAZERT; Michal JEŠETA; Katarzyna OZEGOWSKA; Patrycja SUJKA-KORDOWSKA; Aneta KONWERSKA; Artur BRYJA; Wieslawa KRANC; Maurycy JANKOWSKI; Mariusz J. NAWROCKI; Ievgeniia KOCHEROVA; Piotr CELICHOWSKI; Blanka BOROWIEC; Malgorzata POPIS; Joanna BUDNA-TUKAN; Pawel ANTOSIK; Dorota BUKOWSKA; Klaus P. BRUSSOW; Leszek PAWELCZYK; Malgorzata BRUSKA; Maciej ZABEL; Michal NOWICKI a Bartosz KEMPISTY. The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study. International Journal of Molecular Sciences. Basel, Switzerland: MDPI AG, 2019, roč. 20, č. 1, s. 1-21. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms20010084.

@article{1605582,
   author = {Chermula, Blazej and Brazert, Maciej and Ješeta, Michal and Ozegowska, Katarzyna and SujkaandKordowska, Patrycja and Konwerska, Aneta and Bryja, Artur and Kranc, Wieslawa and Jankowski, Maurycy and Nawrocki, Mariusz J. and Kocherova, Ievgeniia and Celichowski, Piotr and Borowiec, Blanka and Popis, Malgorzata and BudnaandTukan, Joanna and Antosik, Pawel and Bukowska, Dorota and Brussow, Klaus P. and Pawelczyk, Leszek and Bruska, Malgorzata and Zabel, Maciej and Nowicki, Michal and Kempisty, Bartosz},
   article_location = {Basel, Switzerland},
   article_number = {1},
   doi = {https://doi.org/10.3390/ijms20010084},
   keywords = {pig; oocytes; microarray; cellular competence},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study},
   url = {http://dx.doi.org/10.3390/ijms20010084},
   volume = {20},
   year = {2019}
}

TY  - JOUR
ID  - 1605582
AU  - Chermula, Blazej - Brazert, Maciej - Ješeta, Michal - Ozegowska, Katarzyna - Sujka-Kordowska, Patrycja - Konwerska, Aneta - Bryja, Artur - Kranc, Wieslawa - Jankowski, Maurycy - Nawrocki, Mariusz J. - Kocherova, Ievgeniia - Celichowski, Piotr - Borowiec, Blanka - Popis, Malgorzata - Budna-Tukan, Joanna - Antosik, Pawel - Bukowska, Dorota - Brussow, Klaus P. - Pawelczyk, Leszek - Bruska, Malgorzata - Zabel, Maciej - Nowicki, Michal - Kempisty, Bartosz
PY  - 2019
TI  - The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study
JF  - International Journal of Molecular Sciences
VL  - 20
IS  - 1
SP  - 1-21
EP  - 1-21
PB  - MDPI AG
SN  - 14220067
KW  - pig
KW  - oocytes
KW  - microarray
KW  - cellular competence
UR  - http://dx.doi.org/10.3390/ijms20010084
L2  - http://dx.doi.org/10.3390/ijms20010084
N2  - The growth and development of oocyte affect the functional activities of the surrounding somatic cells. These cells are regulated by various types of hormones, proteins, metabolites, and regulatory molecules through gap communication, ultimately leading to the development and maturation of oocytes. The close association between somatic cells and oocytes, which together form the cumulus-oocyte complexes (COCs), and their bi-directional communication are crucial for the acquisition of developmental competences by the oocyte. In this study, oocytes were extracted from the ovaries obtained from crossbred landrace gilts and subjected to in vitro maturation. RNA isolated from those oocytes was used for the subsequent microarray analysis. The data obtained shows, for the first time, variable levels of gene expression (fold changes higher than |2| and adjusted p-value < 0.05) belonging to four ontological groups: regulation of cell proliferation (GO:0042127), regulation of cell migration (GO:0030334), and regulation of programmed cell death (GO:0043067) that can be used together as proliferation, migration or apoptosis markers. We have identified several genes of porcine oocytes (ID2, VEGFA, BTG2, ESR1, CCND2, EDNRA, ANGPTL4, TGFBR3, GJA1, LAMA2, KIT, TPM1, VCP, GRID2, MEF2C, RPS3A, PLD1, BTG3, CD47, MITF), whose expression after in vitro maturation (IVM) is downregulated with different degrees. Our results may be helpful in further elucidating the molecular basis and functional significance of a number of gene markers associated with the processes of migration, proliferation and angiogenesis occurring in COCs.
ER  -

CHERMULA, Blazej; Maciej BRAZERT; Michal JEŠETA; Katarzyna OZEGOWSKA; Patrycja SUJKA-KORDOWSKA; Aneta KONWERSKA; Artur BRYJA; Wieslawa KRANC; Maurycy JANKOWSKI; Mariusz J. NAWROCKI; Ievgeniia KOCHEROVA; Piotr CELICHOWSKI; Blanka BOROWIEC; Malgorzata POPIS; Joanna BUDNA-TUKAN; Pawel ANTOSIK; Dorota BUKOWSKA; Klaus P. BRUSSOW; Leszek PAWELCZYK; Malgorzata BRUSKA; Maciej ZABEL; Michal NOWICKI a Bartosz KEMPISTY. The Unique Mechanisms of Cellular Proliferation, Migration and Apoptosis are Regulated through Oocyte Maturational DevelopmentA Complete Transcriptomic and Histochemical Study. \textit{International Journal of Molecular Sciences}. Basel, Switzerland: MDPI AG, 2019, roč.~20, č.~1, s.~1-21. ISSN~1422-0067. Dostupné z: https://doi.org/10.3390/ijms20010084.

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