Newly identified DNA methyltransferases of Ixodes ricinus ticks

J 2020

Newly identified DNA methyltransferases of Ixodes ricinus ticks

KOTSARENKO, Kateryna; Pavlina VECHTOVA; Zuzana HAMMEROVÁ; Natália LANGOVÁ; Lenka MALINOVSKÁ et al.

Základní údaje

Originální název

Newly identified DNA methyltransferases of Ixodes ricinus ticks

Autoři

KOTSARENKO, Kateryna; Pavlina VECHTOVA; Zuzana HAMMEROVÁ; Natália LANGOVÁ; Lenka MALINOVSKÁ ; Michaela WIMMEROVÁ; Jan STERBA a Libor GRUBHOFFER

Vydání

Ticks and Tick-borne Diseases, Munich, Elsevier GmbH, 2020, 1877-959X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.744

Kód RIV

RIV/00216224:14740/20:00115233

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1016/j.ttbdis.2019.101348

UT WoS

000509982500020

EID Scopus

2-s2.0-85076253184

Klíčová slova anglicky

DNA methylation; Methyltransferase; 5-Methylcytosine; N6-Methyladenine; Epigenetics; Ixodes tick

Štítky

14313050, 143160, CF BIC, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2024 15:00, Ing. Martina Blahová

Anotace

V originále

DNA methylation at the fifth position of cytosine (5mC) and at the sixth position of adenine (6 mA) plays an important role in the regulation of the gene expression and, in eukaryotes, is essential for normal development. For Ixodes ricinus, the most common European arthropod vector of human and animal pathogens, the DNA methylation profile and the role of DNA methylation in tick development are still under discussion. Our goal was to analyze the status of I. ricinus DNA methylation at different life stages and identify enzymes that produce this type of DNA modification. We found that 5mC and 6mA are present in I. ricinus genomic DNA at all life stages. In the transcriptome of I. ricinus, we identified the sequences of the putative IrDNMT1, IrDNMT3, and IrDAMT enzymes, and bioinformatic analysis and three-dimensional modeling predicted their DNA methylation activity. This confirms that I. ricinus possesses a complete DNA methylation toolkit. Our results suggest that DNA methylation is important for the physiology and transstadial development of ticks.

Návaznosti

LM2015055, projekt VaV

Název: Centrum pro systémovou biologii (Akronym: C4SYS)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, The national infrastructure C4SYS - Centre for Systems Biology

LM2015085, projekt VaV

Název: CERIT Scientific Cloud (Akronym: CERIT-SC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CERIT Scientific Cloud

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90042, velká výzkumná infrastruktura

Název: CESNET II

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

KOTSARENKO, Kateryna; Pavlina VECHTOVA; Zuzana HAMMEROVÁ; Natália LANGOVÁ; Lenka MALINOVSKÁ; Michaela WIMMEROVÁ; Jan STERBA a Libor GRUBHOFFER. Newly identified DNA methyltransferases of Ixodes ricinus ticks. Ticks and Tick-borne Diseases. Munich: Elsevier GmbH, 2020, roč. 11, č. 2, s. 101348-101357. ISSN 1877-959X. Dostupné z: https://doi.org/10.1016/j.ttbdis.2019.101348.

@article{1608904,
   author = {Kotsarenko, Kateryna and Vechtova, Pavlina and Hammerová, Zuzana and Langová, Natália and Malinovská, Lenka and Wimmerová, Michaela and Sterba, Jan and Grubhoffer, Libor},
   article_location = {Munich},
   article_number = {2},
   doi = {https://doi.org/10.1016/j.ttbdis.2019.101348},
   keywords = {DNA methylation; Methyltransferase; 5-Methylcytosine; N6-Methyladenine; Epigenetics; Ixodes tick},
   language = {eng},
   issn = {1877-959X},
   journal = {Ticks and Tick-borne Diseases},
   title = {Newly identified DNA methyltransferases of Ixodes ricinus ticks},
   url = {https://www.sciencedirect.com/science/article/pii/S1877959X19301992?via%3Dihub},
   volume = {11},
   year = {2020}
}

TY  - JOUR
ID  - 1608904
AU  - Kotsarenko, Kateryna - Vechtova, Pavlina - Hammerová, Zuzana - Langová, Natália - Malinovská, Lenka - Wimmerová, Michaela - Sterba, Jan - Grubhoffer, Libor
PY  - 2020
TI  - Newly identified DNA methyltransferases of Ixodes ricinus ticks
JF  - Ticks and Tick-borne Diseases
VL  - 11
IS  - 2
SP  - 101348
EP  - 101348
PB  - Elsevier GmbH
SN  - 1877959X
KW  - DNA methylation
KW  - Methyltransferase
KW  - 5-Methylcytosine
KW  - N6-Methyladenine
KW  - Epigenetics
KW  - Ixodes tick
UR  - https://www.sciencedirect.com/science/article/pii/S1877959X19301992?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S1877959X19301992?via%3Dihub
N2  - DNA methylation at the fifth position of cytosine (5mC) and at the sixth position of adenine (6 mA) plays an important role in the regulation of the gene expression and, in eukaryotes, is essential for normal development. For Ixodes ricinus, the most common European arthropod vector of human and animal pathogens, the DNA methylation profile and the role of DNA methylation in tick development are still under discussion. Our goal was to analyze the status of I. ricinus DNA methylation at different life stages and identify enzymes that produce this type of DNA modification. We found that 5mC and 6mA are present in I. ricinus genomic DNA at all life stages. In the transcriptome of I. ricinus, we identified the sequences of the putative IrDNMT1, IrDNMT3, and IrDAMT enzymes, and bioinformatic analysis and three-dimensional modeling predicted their DNA methylation activity. This confirms that I. ricinus possesses a complete DNA methylation toolkit. Our results suggest that DNA methylation is important for the physiology and transstadial development of ticks.
ER  -

KOTSARENKO, Kateryna; Pavlina VECHTOVA; Zuzana HAMMEROVÁ; Natália LANGOVÁ; Lenka MALINOVSKÁ; Michaela WIMMEROVÁ; Jan STERBA a Libor GRUBHOFFER. Newly identified DNA methyltransferases of Ixodes ricinus ticks. \textit{Ticks and Tick-borne Diseases}. Munich: Elsevier GmbH, 2020, roč.~11, č.~2, s.~101348-101357. ISSN~1877-959X. Dostupné z: https://doi.org/10.1016/j.ttbdis.2019.101348.

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