Application of the Enzymatic Electrochemical Biosensors for
Monitoring Non-Competitive Inhibition of Enzyme Activity by
Heavy Metals

J 2019

Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals

ASHRAFI, A.M., M. SYS, E. SEDLACKOVA, A.S. FARAG, V. ADAM et. al.

Základní údaje

Originální název

Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals

Autoři

ASHRAFI, A.M. (203 Česká republika), M. SYS (203 Česká republika), E. SEDLACKOVA (203 Česká republika), A.S. FARAG (203 Česká republika), V. ADAM (203 Česká republika), Jan PŘIBYL (203 Česká republika, garant, domácí) a L. RICHTERA (203 Česká republika)

Vydání

Sensors, Basel, MDPI AG, 2019, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.275

Kód RIV

RIV/00216224:14740/19:00112781

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/s19132939

UT WoS

000477045000100

Klíčová slova anglicky

glucose oxidase; heavy metals; amperometric biosensor; non-competitive inhibition

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 10. 2024 12:58, Mgr. Adéla Pešková

Anotace

V originále

The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion((R)) layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.

Návaznosti

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

ASHRAFI, A.M., M. SYS, E. SEDLACKOVA, A.S. FARAG, V. ADAM, Jan PŘIBYL a L. RICHTERA. Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals. Sensors. Basel: MDPI AG, 2019, roč. 19, č. 13, s. 2939-2952. ISSN 1424-8220. Dostupné z: https://dx.doi.org/10.3390/s19132939.

@article{1612117,
   author = {Ashrafi, A.M. and Sys, M. and Sedlackova, E. and Farag, A.S. and Adam, V. and Přibyl, Jan and Richtera, L.},
   article_location = {Basel},
   article_number = {13},
   doi = {http://dx.doi.org/10.3390/s19132939},
   keywords = {glucose oxidase; heavy metals; amperometric biosensor; non-competitive inhibition},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals},
   url = {https://www.mdpi.com/1424-8220/19/13/2939},
   volume = {19},
   year = {2019}
}

TY  - JOUR
ID  - 1612117
AU  - Ashrafi, A.M. - Sys, M. - Sedlackova, E. - Farag, A.S. - Adam, V. - Přibyl, Jan - Richtera, L.
PY  - 2019
TI  - Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals
JF  - Sensors
VL  - 19
IS  - 13
SP  - 2939
EP  - 2939
PB  - MDPI AG
SN  - 14248220
KW  - glucose oxidase
KW  - heavy metals
KW  - amperometric biosensor
KW  - non-competitive inhibition
UR  - https://www.mdpi.com/1424-8220/19/13/2939
L2  - https://www.mdpi.com/1424-8220/19/13/2939
N2  - The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion((R)) layer was used for immobilization of Gox. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of Gox. Therefore, the development of Gox biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.
ER  -

ASHRAFI, A.M., M. SYS, E. SEDLACKOVA, A.S. FARAG, V. ADAM, Jan PŘIBYL a L. RICHTERA. Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals. \textit{Sensors}. Basel: MDPI AG, 2019, roč.~19, č.~13, s.~2939-2952. ISSN~1424-8220. Dostupné z: https://dx.doi.org/10.3390/s19132939.

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