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@article{1614685,
author = {Brazert, Maciej and Kranc, Wieslawa and Chermula, Blazej and Kowalska, Katarzyna and Jankowski, Maurycy and Celichowski, Piotr and Ješeta, Michal and PiotrowskaandKempisty, Hanna and Pawelczyk, Leszek and Zabel, Maciej and Mozdziak, Paul and Kempisty, Bartosz},
article_location = {BASEL},
article_number = {12},
doi = {http://dx.doi.org/10.3390/jcm8122026},
keywords = {ovarian granulosa; human; in vitro; cell division},
language = {eng},
issn = {2077-0383},
journal = {Journal of Clinical Medicine},
title = {Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation},
url = {http://dx.doi.org/10.3390/jcm8122026},
volume = {8},
year = {2019}
}
TY - JOUR
ID - 1614685
AU - Brazert, Maciej - Kranc, Wieslawa - Chermula, Blazej - Kowalska, Katarzyna - Jankowski, Maurycy - Celichowski, Piotr - Ješeta, Michal - Piotrowska-Kempisty, Hanna - Pawelczyk, Leszek - Zabel, Maciej - Mozdziak, Paul - Kempisty, Bartosz
PY - 2019
TI - Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation
JF - Journal of Clinical Medicine
VL - 8
IS - 12
SP - 1-18
EP - 1-18
PB - MDPI
SN - 20770383
KW - ovarian granulosa
KW - human
KW - in vitro
KW - cell division
UR - http://dx.doi.org/10.3390/jcm8122026
L2 - http://dx.doi.org/10.3390/jcm8122026
N2 - Granulosa cells (GCs) are a population of somatic cells whose role after ovulation is progesterone production. GCs were collected from patients undergoing controlled ovarian stimulation during an in vitro fertilization procedure, and they were maintained for 1, 7, 15, and 30 days of in vitro primary culture before collection for further gene expression analysis. A study of genes involved in the biological processes of interest was carried out using expression microarrays. To validate the obtained results, Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) was performed. The direction of changes in the expression of the selected genes was confirmed in most of the examples. Six ontological groups ("cell cycle arrest", "cell cycle process", "mitotic spindle organization", "mitotic spindle assembly checkpoint", "mitotic spindle assembly", and "mitotic spindle checkpoint") were analyzed in this study. The results of the microarrays obtained by us allowed us to identify two groups of genes whose expressions were the most upregulated (FAM64A, ANLN, TOP2A, CTGF, CEP55, BIRC5, PRC1, DLGAP5, GAS6, and NDRG1) and the most downregulated (EREG, PID1, INHA, RHOU, CXCL8, SEPT6, EPGN, RDX, WNT5A, and EZH2) during the culture. The cellular ultrastructure showed the presence of structures characteristic of mitotic cell division: a centrosome surrounded by a pericentric matrix, a microtubule system, and a mitotic spindle connected to chromosomes. The main goal of the study was to identify the genes involved in mitotic division and to identify the cellular ultrastructure of GCs in a long-term in vitro culture. All of the genes in these groups were subjected to downstream analysis, and their function and relation to the ovarian environment are discussed. The obtained results suggest that long-term in vitro cultivation of GCs may lead to their differentiation toward another cell type, including cells with cancer-like characteristics.
ER -
BRAZERT, Maciej, Wieslawa KRANC, Blazej CHERMULA, Katarzyna KOWALSKA, Maurycy JANKOWSKI, Piotr CELICHOWSKI, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Leszek PAWELCZYK, Maciej ZABEL, Paul MOZDZIAK a Bartosz KEMPISTY. Human Ovarian Granulosa Cells Isolated during an IVF Procedure Exhibit Differential Expression of Genes Regulating Cell Division and Mitotic Spindle Formation. \textit{Journal of Clinical Medicine}. BASEL: MDPI, 2019, roč.~8, č.~12, s.~1-18. ISSN~2077-0383. Dostupné z: https://dx.doi.org/10.3390/jcm8122026.
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