J 2019

EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System

DONGEN VAN, Jacques J. M., Mirjam BURG VAN DER, Tomas KALINA, Martin PEREZ-ANDRES, Ester MEJSTRIKOVA et. al.

Základní údaje

Originální název

EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System

Autoři

DONGEN VAN, Jacques J. M. (528 Nizozemské království, garant), Mirjam BURG VAN DER (528 Nizozemské království), Tomas KALINA (203 Česká republika), Martin PEREZ-ANDRES (724 Španělsko), Ester MEJSTRIKOVA (203 Česká republika), Marcela VLKOVÁ (203 Česká republika, domácí), Eduardo LOPEZ-GRANADOS (724 Španělsko), Marjolein WENTINK (528 Nizozemské království), Anne-Kathrin KIENZLER (826 Velká Británie a Severní Irsko), Jan PHILIPPE (56 Belgie), Ana E. SOUSA (620 Portugalsko), Menno C. ZELM VAN (528 Nizozemské království), Elena BLANCO (724 Španělsko) a Alberto ORFAO (724 Španělsko)

Vydání

Frontiers in Immunology, LAUSANNE, FRONTIERS MEDIA SA, 2019, 1664-3224

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 5.085

Kód RIV

RIV/00216224:14110/19:00112952

Organizační jednotka

Lékařská fakulta

DOI

UT WoS

000471898900001

Klíčová slova anglicky

immunodeficiency; immunophenotyping; flow cytometry; diagnosis; classification; EuroFlow; standardization

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 17. 2. 2020 07:57, Mgr. Tereza Miškechová

Anotace

V originále

Guidelines for screening for primary immunodeficiencies (PID) are well-defined and several consensus diagnostic strategies have been proposed. These consensus proposals have only partially been implemented due to lack of standardization in laboratory procedures, particularly in flow cytometry. The main objectives of the EuroFlow Consortium were to innovate and thoroughly standardize the flowcytometric techniques and strategies for reliable and reproducible diagnosis and classification of PID of the lymphoid system. The proposed EuroFlow antibody panels comprise one orientation tube and seven classification tubes and corresponding databases of normal and PID samples. The 8-color 12-antibody PID Orientation tube (PIDOT) aims at identification and enumeration of the main lymphocyte and leukocyte subsets; this includes naive pre-germinal center (GC) and antigen-experienced post-GC memory B-cells and plasmablasts. The seven additional 8(-12)-color tubes can be used according to the EuroFlow PID algorithm in parallel or subsequently to the PIDOT for more detailed analysis of B-cell and T-cell subsets to further classify PID of the lymphoid system. The Pre-GC, Post-GC, and immunoglobulin heavy chain (IgH)-isotype B-cell tubes aim at identification and enumeration of B-cell subsets for evaluation of B-cell maturation blocks and specific defects in IgH-subclass production. The severe combined immunodeficiency (SCID) tube and T-cell memory/effector subset tube aim at identification and enumeration of T-cell subsets for assessment of T-cell defects, such as SCID. In case of suspicion of antibody deficiency, PIDOT is preferably directly combined with the IgH isotype tube(s) and in case of SCID suspicion (e.g., in newborn screening programs) the PIDOT is preferably directly combined with the SCID T-cell tube. The proposed >= 8-color antibody panels and corresponding reference databases combined with the EuroFlow PID algorithm are designed to provide fast, sensitive and cost-effective flowcytometric diagnosis of PID of the lymphoid system, easily applicable in multicenter diagnostic settings world-wide.