BUDNA-TUKAN, Joanna, Agata SWIATLY-BLASZKIEWICZ, Piotr CELICHOWSKI, Sandra KALUZNA, Aneta KONWERSKA, Patrycja SUJKA-KORDOWSKA, Maurycy JANKOWSKI, Magdalena KULUS, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Malgorzata JOZKOWIAK, Pawel ANTOSIK, Dorota BUKOWSKA, Mariusz T. SKOWRONSKI, Jan MATYSIAK, Michal NOWICKI a Bartosz KEMPISTY. "Biological Adhesion" Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, roč. 20, č. 14, s. 1-18. ISSN 1422-0067. doi:10.3390/ijms20143387. 2019.
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Základní údaje
Originální název "Biological Adhesion" Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study
Autoři BUDNA-TUKAN, Joanna (616 Polsko, garant), Agata SWIATLY-BLASZKIEWICZ (616 Polsko), Piotr CELICHOWSKI (616 Polsko), Sandra KALUZNA (616 Polsko), Aneta KONWERSKA (616 Polsko), Patrycja SUJKA-KORDOWSKA (616 Polsko), Maurycy JANKOWSKI (616 Polsko), Magdalena KULUS (616 Polsko), Michal JEŠETA (203 Česká republika, domácí), Hanna PIOTROWSKA-KEMPISTY (616 Polsko), Malgorzata JOZKOWIAK (616 Polsko), Pawel ANTOSIK (616 Polsko), Dorota BUKOWSKA (616 Polsko), Mariusz T. SKOWRONSKI (616 Polsko), Jan MATYSIAK (616 Polsko), Michal NOWICKI (616 Polsko) a Bartosz KEMPISTY (616 Polsko).
Vydání International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2019, 1422-0067.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10608 Biochemistry and molecular biology
Stát vydavatele Švýcarsko
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 4.556
Kód RIV RIV/00216224:14110/19:00112990
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.3390/ijms20143387
UT WoS 000480449300012
Klíčová slova anglicky oviductal epithelial cells; biological adhesion; microarray; mass spectrometry; pig
Štítky 14110411, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Tereza Miškechová, učo 341652. Změněno: 21. 7. 2021 10:30.
Anotace
Oviductal epithelial cells (OECs) actively produce stimulating and protecting factors, favoring survival and viability of gametes and early embryos. The oviduct participates in the initial reproductive events, which strongly depends on adhesion. The analysis of differential gene expression in OECs, during long-term in vitro culture, enables recognition of new molecular markers regulating several processes, including "biological adhesion". Porcine oviducts were stained with hematoxylin and eosin, as well as with antibodies against epithelial markers. Then, OECs were long-term in vitro cultured and after 24 h, 7, 15, and 30 days of culture were subjected to transcriptomic and proteomic assays. Microarrays were employed to evaluate gene expression, with Matrix-assisted laser desorption/ionization-time of light (MALDI-TOF) mass spectrometry applied to determine the proteome. The results revealed proper morphology of the oviducts and typical epithelial structure of OECs during the culture. From the set of differentially expressed genes (DEGs), we have selected the 130 that encoded proteins detected by MALDI-TOF MS analysis. From this gene pool, 18 significantly enriched gene ontology biological processes (GO BP) terms were extracted. Among them we focused on genes belonging to "biological adhesion" GO BP. It is suggested that increased expression of studied genes can be attributed to the process of intensive secretion of substances that exhibit favorable influence on oviductal environment, which prime gametes adhesion and viability, fertilization, and early embryo journey.
VytisknoutZobrazeno: 29. 3. 2024 05:34