"Biological Adhesion" Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study

BUDNA-TUKAN, Joanna; Agata SWIATLY-BLASZKIEWICZ; Piotr CELICHOWSKI; Sandra KALUZNA; Aneta KONWERSKA; Patrycja SUJKA-KORDOWSKA; Maurycy JANKOWSKI; Magdalena KULUS; Michal JEŠETA; Hanna PIOTROWSKA-KEMPISTY; Malgorzata JOZKOWIAK; Pawel ANTOSIK; Dorota BUKOWSKA; Mariusz T. SKOWRONSKI; Jan MATYSIAK; Michal NOWICKI a Bartosz KEMPISTY. "Biological Adhesion" Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2019, roč. 20, č. 14, s. 1-18. ISSN 1422-0067. Dostupné z: https://doi.org/10.3390/ijms20143387.

Základní údaje

Originální název

"Biological Adhesion" Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study

Autoři

BUDNA-TUKAN, Joanna; Agata SWIATLY-BLASZKIEWICZ; Piotr CELICHOWSKI; Sandra KALUZNA; Aneta KONWERSKA; Patrycja SUJKA-KORDOWSKA; Maurycy JANKOWSKI; Magdalena KULUS; Michal JEŠETA; Hanna PIOTROWSKA-KEMPISTY; Malgorzata JOZKOWIAK; Pawel ANTOSIK; Dorota BUKOWSKA; Mariusz T. SKOWRONSKI; Jan MATYSIAK; Michal NOWICKI a Bartosz KEMPISTY

Vydání

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2019, 1422-0067

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/19:00112990

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.3390/ijms20143387

UT WoS

000480449300012

EID Scopus

2-s2.0-85069787521

Klíčová slova anglicky

oviductal epithelial cells; biological adhesion; microarray; mass spectrometry; pig

Štítky

14110411, rivok

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

Oviductal epithelial cells (OECs) actively produce stimulating and protecting factors, favoring survival and viability of gametes and early embryos. The oviduct participates in the initial reproductive events, which strongly depends on adhesion. The analysis of differential gene expression in OECs, during long-term in vitro culture, enables recognition of new molecular markers regulating several processes, including "biological adhesion". Porcine oviducts were stained with hematoxylin and eosin, as well as with antibodies against epithelial markers. Then, OECs were long-term in vitro cultured and after 24 h, 7, 15, and 30 days of culture were subjected to transcriptomic and proteomic assays. Microarrays were employed to evaluate gene expression, with Matrix-assisted laser desorption/ionization-time of light (MALDI-TOF) mass spectrometry applied to determine the proteome. The results revealed proper morphology of the oviducts and typical epithelial structure of OECs during the culture. From the set of differentially expressed genes (DEGs), we have selected the 130 that encoded proteins detected by MALDI-TOF MS analysis. From this gene pool, 18 significantly enriched gene ontology biological processes (GO BP) terms were extracted. Among them we focused on genes belonging to "biological adhesion" GO BP. It is suggested that increased expression of studied genes can be attributed to the process of intensive secretion of substances that exhibit favorable influence on oviductal environment, which prime gametes adhesion and viability, fertilization, and early embryo journey.