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@article{1624016, author = {BudnaandTukan, Joanna and SwiatlyandBlaszkiewicz, Agata and Celichowski, Piotr and Kaluzna, Sandra and Konwerska, Aneta and SujkaandKordowska, Patrycja and Jankowski, Maurycy and Kulus, Magdalena and Ješeta, Michal and PiotrowskaandKempisty, Hanna and Jozkowiak, Malgorzata and Antosik, Pawel and Bukowska, Dorota and Skowronski, Mariusz T. and Matysiak, Jan and Nowicki, Michal and Kempisty, Bartosz}, article_location = {Basel}, article_number = {14}, doi = {http://dx.doi.org/10.3390/ijms20143387}, keywords = {oviductal epithelial cells; biological adhesion; microarray; mass spectrometry; pig}, language = {eng}, issn = {1422-0067}, journal = {International Journal of Molecular Sciences}, title = {"Biological Adhesion" Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study}, url = {http://dx.doi.org/10.3390/ijms20143387}, volume = {20}, year = {2019} }
TY - JOUR ID - 1624016 AU - Budna-Tukan, Joanna - Swiatly-Blaszkiewicz, Agata - Celichowski, Piotr - Kaluzna, Sandra - Konwerska, Aneta - Sujka-Kordowska, Patrycja - Jankowski, Maurycy - Kulus, Magdalena - Ješeta, Michal - Piotrowska-Kempisty, Hanna - Jozkowiak, Malgorzata - Antosik, Pawel - Bukowska, Dorota - Skowronski, Mariusz T. - Matysiak, Jan - Nowicki, Michal - Kempisty, Bartosz PY - 2019 TI - "Biological Adhesion" Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study JF - International Journal of Molecular Sciences VL - 20 IS - 14 SP - 1-18 EP - 1-18 PB - Multidisciplinary Digital Publishing Institute SN - 14220067 KW - oviductal epithelial cells KW - biological adhesion KW - microarray KW - mass spectrometry KW - pig UR - http://dx.doi.org/10.3390/ijms20143387 L2 - http://dx.doi.org/10.3390/ijms20143387 N2 - Oviductal epithelial cells (OECs) actively produce stimulating and protecting factors, favoring survival and viability of gametes and early embryos. The oviduct participates in the initial reproductive events, which strongly depends on adhesion. The analysis of differential gene expression in OECs, during long-term in vitro culture, enables recognition of new molecular markers regulating several processes, including "biological adhesion". Porcine oviducts were stained with hematoxylin and eosin, as well as with antibodies against epithelial markers. Then, OECs were long-term in vitro cultured and after 24 h, 7, 15, and 30 days of culture were subjected to transcriptomic and proteomic assays. Microarrays were employed to evaluate gene expression, with Matrix-assisted laser desorption/ionization-time of light (MALDI-TOF) mass spectrometry applied to determine the proteome. The results revealed proper morphology of the oviducts and typical epithelial structure of OECs during the culture. From the set of differentially expressed genes (DEGs), we have selected the 130 that encoded proteins detected by MALDI-TOF MS analysis. From this gene pool, 18 significantly enriched gene ontology biological processes (GO BP) terms were extracted. Among them we focused on genes belonging to "biological adhesion" GO BP. It is suggested that increased expression of studied genes can be attributed to the process of intensive secretion of substances that exhibit favorable influence on oviductal environment, which prime gametes adhesion and viability, fertilization, and early embryo journey. ER -
BUDNA-TUKAN, Joanna, Agata SWIATLY-BLASZKIEWICZ, Piotr CELICHOWSKI, Sandra KALUZNA, Aneta KONWERSKA, Patrycja SUJKA-KORDOWSKA, Maurycy JANKOWSKI, Magdalena KULUS, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Malgorzata JOZKOWIAK, Pawel ANTOSIK, Dorota BUKOWSKA, Mariusz T. SKOWRONSKI, Jan MATYSIAK, Michal NOWICKI and Bartosz KEMPISTY. ''Biological Adhesion'' Is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2019, vol.~20, No~14, p.~1-18. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms20143387.
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