"Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long-term in vitro cultivation-a microarray approach

KRANC, Wieslawa; Maciej BRAZERT; Piotr CELICHOWSKI; Artur BRYJA; Mariusz J. NAWROCKI; Katarzyna OZEGOWSKA; Maurycy JANKOWSKI; Michal JEŠETA; Leszek PAWELCZYK; Andrzej BREBOROWICZ; Dominik RACHON; Mariusz T. SKOWRONSKI; Malgorzata BRUSKA; Maciej ZABEL; Michal NOWICKI a Bartosz KEMPISTY. "Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long-term in vitro cultivation-a microarray approach. Molecular Medicine Reports. ATHENS: SPANDIDOS PUBL LTD, 2019, roč. 19, č. 3, s. 1705-1715. ISSN 1791-2997. Dostupné z: https://doi.org/10.3892/mmr.2019.9837.

Základní údaje

Originální název

"Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long-term in vitro cultivation-a microarray approach

Autoři

KRANC, Wieslawa; Maciej BRAZERT; Piotr CELICHOWSKI; Artur BRYJA; Mariusz J. NAWROCKI; Katarzyna OZEGOWSKA; Maurycy JANKOWSKI; Michal JEŠETA; Leszek PAWELCZYK; Andrzej BREBOROWICZ; Dominik RACHON; Mariusz T. SKOWRONSKI; Malgorzata BRUSKA; Maciej ZABEL; Michal NOWICKI a Bartosz KEMPISTY

Vydání

Molecular Medicine Reports, ATHENS, SPANDIDOS PUBL LTD, 2019, 1791-2997

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.100

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/19:00112995

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.3892/mmr.2019.9837

UT WoS

000459710100031

EID Scopus

2-s2.0-85061127169

Klíčová slova anglicky

human granulosa cells; in vitro culture; proliferation; differentiation; heart development

Štítky

14110411, rivok

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

Granulosa cells (GCs) have many functions in the endocrine system. Most notably, they produce progesterone following ovulation. However, it has recently been proven that GCs can change their properties when subjected to long-term culture. In the present study, GCs were collected from hyper-stimulated ovarian follicles during in vitro fertilization procedures. They were grown in vitro, in a long-term manner. RNA was collected following 1, 7, 15 and 30 days of culture. Expression microarrays were used for analysis, which allowed to identify groups of genes characteristic for particular cellular processes. In addition, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to validate the obtained results. Two ontological groups characteristic for processes associated with the development and morphogenesis of the heart were identified during the analyses: Heart development' and heart morphogenesis'. The results of the microarrays revealed that the highest change in expression was demonstrated by the lysyl Oxidase, oxytocin receptor, nexilin F-actin binding protein, and cysteine-rich protein 3 genes. The lowest change was exhibited by odd-skipped related transcription factor 1, plakophilin 2, transcription growth factor- receptor 1, and kinesin family member 3A. The direction of changes was confirmed by RT-qPCR results. In the present study, it was suggested that GCs may have the potential to differentiate towards other cell types under long-term in vitro culture conditions. Thus, genes belonging to the presented ontological groups can be considered as novel markers of proliferation and differentiation of GCs towards the heart muscle cells.