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@article{1624716,
author = {Chermula, B. and Brazert, M. and Izycki, D. and Ciesiolka, S. and Kranc, W. and Celichowski, P. and Ozegowska, K. and Nawrocki, M. J. and Jankowski, M. and Ješeta, Michal and Antosik, P. and Bukowska, D. and Skowronski, M. T. and Brussow, K. P. and Bruska, M. and Pawelczyk, L. and Zabel, M. and Nowicki, M. and Kempisty, B.},
article_location = {New York},
article_number = {2019},
doi = {http://dx.doi.org/10.1155/2019/6545210},
keywords = {INDIAN HEDGEHOG; GROWTH; DIFFERENTIATION; DEFICIENCY; ACTIVATION; PREGNANCY; STEMNESS; MATRIX; MICE},
language = {eng},
issn = {2314-6133},
journal = {Biomed Research International},
title = {New Gene Markers of Angiogenesis and Blood Vessels Development in Porcine Ovarian Granulosa Cells during Short-Term Primary Culture In Vitro},
url = {http://dx.doi.org/10.1155/2019/6545210},
volume = {2019},
year = {2019}
}
TY - JOUR
ID - 1624716
AU - Chermula, B. - Brazert, M. - Izycki, D. - Ciesiolka, S. - Kranc, W. - Celichowski, P. - Ozegowska, K. - Nawrocki, M. J. - Jankowski, M. - Ješeta, Michal - Antosik, P. - Bukowska, D. - Skowronski, M. T. - Brussow, K. P. - Bruska, M. - Pawelczyk, L. - Zabel, M. - Nowicki, M. - Kempisty, B.
PY - 2019
TI - New Gene Markers of Angiogenesis and Blood Vessels Development in Porcine Ovarian Granulosa Cells during Short-Term Primary Culture In Vitro
JF - Biomed Research International
VL - 2019
IS - 2019
SP - 1-12
EP - 1-12
PB - Hindawi Publishing Corporation
SN - 23146133
KW - INDIAN HEDGEHOG
KW - GROWTH
KW - DIFFERENTIATION
KW - DEFICIENCY
KW - ACTIVATION
KW - PREGNANCY
KW - STEMNESS
KW - MATRIX
KW - MICE
UR - http://dx.doi.org/10.1155/2019/6545210
L2 - http://dx.doi.org/10.1155/2019/6545210
N2 - The physiological processes that drive the development of ovarian follicle, as well as the process of oogenesis, are quite well known. Granulosa cells are major players in this occurrence, being the somatic element of the female gamete development. They participate directly in the processes of oogenesis, building the cumulus-oocyte complex surrounding the ovum. In addition to that, they have a further impact on the reproductive processes, being a place of steroid sex hormone synthesis and secretion. It is known that the follicle development creates a major need for angiogenesis and blood vessel development in the ovary. In this study, we use novel molecular approaches to analyze markers of these processes in porcine granulosa cultured primarily in vitro. The cells were recovered from mature sus scrofa specimen after slaughter. They were then subjected to enzymatic digestion and culture primarily for a short term. The RNA was extracted from cultures in specific time periods (0h, 24h, 48h, 96h, and 144h) and analyzed using expression microarrays. The genes that exhibited fold change bigger than |2|, and adjusted p-value lower than 0.05, were considered differentially expressed. From these, we have chosen the members of angiogenesis, blood vessel development, blood vessel morphogenesis, cardiovascular system development, and vasculature development for further selection. CCL2, FGFR2, SFRP2, PDPN, DCN, CAV1, CHI3L1, ITGB3, FN1, and LOX which are upregulated, as well as CXCL10, NEBL, IHH, TGFBR3, SCUBE1, IGF1, EDNRA, RHOB, PPARD, and SLITRK5 genes whose expression is downregulated through the time of culture, were chosen as the potential markers, as their expression varied the most during the time of culture. The fold changes were further validated with RT-qPCR. The genes were described, with special attention to their possible function in GCs during culture. The results broaden the general knowledge about GC's in vitro molecular processes and might serve as a point of reference for further in vivo and clinical studies.
ER -
CHERMULA, B., M. BRAZERT, D. IZYCKI, S. CIESIOLKA, W. KRANC, P. CELICHOWSKI, K. OZEGOWSKA, M. J. NAWROCKI, M. JANKOWSKI, Michal JEŠETA, P. ANTOSIK, D. BUKOWSKA, M. T. SKOWRONSKI, K. P. BRUSSOW, M. BRUSKA, L. PAWELCZYK, M. ZABEL, M. NOWICKI a B. KEMPISTY. New Gene Markers of Angiogenesis and Blood Vessels Development in Porcine Ovarian Granulosa Cells during Short-Term Primary Culture In Vitro. \textit{Biomed Research International}. New York: Hindawi Publishing Corporation, 2019, roč.~2019, č.~2019, s.~1-12. ISSN~2314-6133. Dostupné z: https://dx.doi.org/10.1155/2019/6545210.
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