Signals trigger state-specific transcriptional programs to
support diversity and homeostasis in immune cells

J 2019

Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells

FISCHER, C.; Maria METSGER; S. BAUCH; R. VIDAL; M. BOTTCHER et al.

Základní údaje

Originální název

Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells

Autoři

FISCHER, C.; Maria METSGER; S. BAUCH; R. VIDAL; M. BOTTCHER; P. GROTE; M. KLIEM a S. SAUER

Vydání

SCIENCE SIGNALING, WASHINGTON, AMER ASSOC ADVANCEMENT SCIENCE, 2019, 1945-0877

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.467

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/19:00113240

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

TOLL-LIKE RECEPTORS; RNA-SEQ; GENE-EXPRESSION; R-PACKAGE; REVEALS; MACROPHAGES; ACTIVATION; MECHANISMS; REGULATOR; SPECTRUM

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 3. 2020 21:53, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Macrophages play key roles in the immune systems of humans and other mammals. Here, we performed single-cell analyses of the mRNAs and proteins of human macrophages to compare their responses to the signaling molecules lipopolysaccharide (LPS), a component of Gram-negative bacteria, and palmitate (PAL), a free fatty acid. We found that, although both molecules signal through the cell surface protein Toll-like receptor 4 (TLR4), they stimulated the expression of different genes, resulting in specific pro- and anti-inflammatory cellular states for each signal. The effects of the glucocorticoid receptor, which antagonizes LPS signaling, and cyclic AMP-dependent transcription factor 3, which inhibits PAL-induced inflammation, on inflammatory response seemed largely determined by digital on-off events. Furthermore, the quantification of transcriptional variance and signaling entropy enabled the identification of cell state-specific deregulated molecular pathways. These data suggest that the preservation of signaling in distinct cells might confer diversity on macrophage populations essential to maintaining major cellular functions.

Citovat

FISCHER, C.; Maria METSGER; S. BAUCH; R. VIDAL; M. BOTTCHER; P. GROTE; M. KLIEM a S. SAUER. Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells. SCIENCE SIGNALING. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE, 2019, roč. 12, č. 581, s. 1-16. ISSN 1945-0877. Dostupné z: https://doi.org/10.1126/scisignal.aao5820.

@article{1635179,
   author = {Fischer, C. and Metsger, Maria and Bauch, S. and Vidal, R. and Bottcher, M. and Grote, P. and Kliem, M. and Sauer, S.},
   article_location = {WASHINGTON},
   article_number = {581},
   doi = {https://doi.org/10.1126/scisignal.aao5820},
   keywords = {TOLL-LIKE RECEPTORS; RNA-SEQ; GENE-EXPRESSION; R-PACKAGE; REVEALS; MACROPHAGES; ACTIVATION; MECHANISMS; REGULATOR; SPECTRUM},
   language = {eng},
   issn = {1945-0877},
   journal = {SCIENCE SIGNALING},
   title = {Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells},
   url = {https://stke.sciencemag.org/content/12/581/eaao5820},
   volume = {12},
   year = {2019}
}

TY  - JOUR
ID  - 1635179
AU  - Fischer, C. - Metsger, Maria - Bauch, S. - Vidal, R. - Bottcher, M. - Grote, P. - Kliem, M. - Sauer, S.
PY  - 2019
TI  - Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells
JF  - SCIENCE SIGNALING
VL  - 12
IS  - 581
SP  - 1-16
EP  - 1-16
PB  - AMER ASSOC ADVANCEMENT SCIENCE
SN  - 19450877
KW  - TOLL-LIKE RECEPTORS
KW  - RNA-SEQ
KW  - GENE-EXPRESSION
KW  - R-PACKAGE
KW  - REVEALS
KW  - MACROPHAGES
KW  - ACTIVATION
KW  - MECHANISMS
KW  - REGULATOR
KW  - SPECTRUM
UR  - https://stke.sciencemag.org/content/12/581/eaao5820
L2  - https://stke.sciencemag.org/content/12/581/eaao5820
N2  - Macrophages play key roles in the immune systems of humans and other mammals. Here, we performed single-cell analyses of the mRNAs and proteins of human macrophages to compare their responses to the signaling molecules lipopolysaccharide (LPS), a component of Gram-negative bacteria, and palmitate (PAL), a free fatty acid. We found that, although both molecules signal through the cell surface protein Toll-like receptor 4 (TLR4), they stimulated the expression of different genes, resulting in specific pro- and anti-inflammatory cellular states for each signal. The effects of the glucocorticoid receptor, which antagonizes LPS signaling, and cyclic AMP-dependent transcription factor 3, which inhibits PAL-induced inflammation, on inflammatory response seemed largely determined by digital on-off events. Furthermore, the quantification of transcriptional variance and signaling entropy enabled the identification of cell state-specific deregulated molecular pathways. These data suggest that the preservation of signaling in distinct cells might confer diversity on macrophage populations essential to maintaining major cellular functions.
ER  -

FISCHER, C.; Maria METSGER; S. BAUCH; R. VIDAL; M. BOTTCHER; P. GROTE; M. KLIEM a S. SAUER. Signals trigger state-specific transcriptional programs to support diversity and homeostasis in immune cells. \textit{SCIENCE SIGNALING}. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE, 2019, roč.~12, č.~581, s.~1-16. ISSN~1945-0877. Dostupné z: https://doi.org/10.1126/scisignal.aao5820.

Přehled o publikaci