A role for the auxin precursor anthranilic acid in root
gravitropism via regulation of PIN-FORMED protein polarity and
relocalisation in Arabidopsis

J 2019

A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis

DOYLE, S.M., A. RIGAL, P. GRONES, M. KARADY, D.K. BARANGE et. al.

Základní údaje

Originální název

A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis

Autoři

DOYLE, S.M., A. RIGAL, P. GRONES, M. KARADY, D.K. BARANGE, M. MAJDA, B. PARIZKOVA, M. KARAMPELIAS, Marta ZWIEWKA (616 Polsko, garant, domácí), A. PENCIK, F. ALMQVIST, K. LJUNG, O. NOVAK a S. ROBERT

Vydání

New Phytologist, HOBOKEN, Blackwell Science, 2019, 0028-646X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.512

Kód RIV

RIV/00216224:14740/19:00108188

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/nph.15877

UT WoS

000475918000032

Klíčová slova anglicky

anthranilic acid (AA); Arabidopsis thaliana; auxin transport; PIN polarity; PIN-FORMED proteins; root gravitropism

Štítky

CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 10. 2024 13:21, Ing. Martina Blahová

Anotace

V originále

distribution of auxin within plant tissues is of great importance for developmental plasticity, including root gravitropic growth. Auxin flow is directed by the subcellular polar distribution and dynamic relocalisation of auxin transporters such as the PIN-FORMED (PIN) efflux carriers, which can be influenced by the main natural plant auxin indole-3-acetic acid (IAA). Anthranilic acid (AA) is an important early precursor of IAA and previously published studies with AA analogues have suggested that AA may also regulate PIN localisation. Using Arabidopsis thaliana as a model species, we studied an AA-deficient mutant displaying agravitropic root growth, treated seedlings with AA and AA analogues and transformed lines to over-produce AA while inhibiting its conversion to downstream IAA precursors. We showed that AA rescues root gravitropic growth in the AA-deficient mutant at concentrations that do not rescue IAA levels. Overproduction of AA affects root gravitropism without affecting IAA levels. Treatments with, or deficiency in, AA result in defects in PIN polarity and gravistimulus-induced PIN relocalisation in root cells. Our results revealed a previously unknown role for AA in the regulation of PIN subcellular localisation and dynamics involved in root gravitropism, which is independent of its better known role in IAA biosynthesis.

Návaznosti

GA13-40637S, projekt VaV

Název: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Grantová agentura ČR, Genetické studie k identifikaci molekulárních mechanizmu bunecné polarity a auxinového transportu v rostlinách

90062, velká výzkumná infrastruktura

Název: Czech-BioImaging

Citovat

DOYLE, S.M., A. RIGAL, P. GRONES, M. KARADY, D.K. BARANGE, M. MAJDA, B. PARIZKOVA, M. KARAMPELIAS, Marta ZWIEWKA, A. PENCIK, F. ALMQVIST, K. LJUNG, O. NOVAK a S. ROBERT. A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis. New Phytologist. HOBOKEN: Blackwell Science, 2019, roč. 223, č. 3, s. 1420-1432. ISSN 0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.15877.

@article{1636883,
   author = {Doyle, S.M. and Rigal, A. and Grones, P. and Karady, M. and Barange, D.K. and Majda, M. and Parizkova, B. and Karampelias, M. and Zwiewka, Marta and Pencik, A. and Almqvist, F. and Ljung, K. and Novak, O. and Robert, S.},
   article_location = {HOBOKEN},
   article_number = {3},
   doi = {http://dx.doi.org/10.1111/nph.15877},
   keywords = {anthranilic acid (AA); Arabidopsis thaliana; auxin transport; PIN polarity; PIN-FORMED proteins; root gravitropism},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis},
   url = {https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15877},
   volume = {223},
   year = {2019}
}

TY  - JOUR
ID  - 1636883
AU  - Doyle, S.M. - Rigal, A. - Grones, P. - Karady, M. - Barange, D.K. - Majda, M. - Parizkova, B. - Karampelias, M. - Zwiewka, Marta - Pencik, A. - Almqvist, F. - Ljung, K. - Novak, O. - Robert, S.
PY  - 2019
TI  - A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis
JF  - New Phytologist
VL  - 223
IS  - 3
SP  - 1420-1432
EP  - 1420-1432
PB  - Blackwell Science
SN  - 0028646X
KW  - anthranilic acid (AA)
KW  - Arabidopsis thaliana
KW  - auxin transport
KW  - PIN polarity
KW  - PIN-FORMED proteins
KW  - root gravitropism
UR  - https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15877
L2  - https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15877
N2  - distribution of auxin within plant tissues is of great importance for developmental plasticity, including root gravitropic growth. Auxin flow is directed by the subcellular polar distribution and dynamic relocalisation of auxin transporters such as the PIN-FORMED (PIN) efflux carriers, which can be influenced by the main natural plant auxin indole-3-acetic acid (IAA). Anthranilic acid (AA) is an important early precursor of IAA and previously published studies with AA analogues have suggested that AA may also regulate PIN localisation. Using Arabidopsis thaliana as a model species, we studied an AA-deficient mutant displaying agravitropic root growth, treated seedlings with AA and AA analogues and transformed lines to over-produce AA while inhibiting its conversion to downstream IAA precursors. We showed that AA rescues root gravitropic growth in the AA-deficient mutant at concentrations that do not rescue IAA levels. Overproduction of AA affects root gravitropism without affecting IAA levels. Treatments with, or deficiency in, AA result in defects in PIN polarity and gravistimulus-induced PIN relocalisation in root cells. Our results revealed a previously unknown role for AA in the regulation of PIN subcellular localisation and dynamics involved in root gravitropism, which is independent of its better known role in IAA biosynthesis.
ER  -

DOYLE, S.M., A. RIGAL, P. GRONES, M. KARADY, D.K. BARANGE, M. MAJDA, B. PARIZKOVA, M. KARAMPELIAS, Marta ZWIEWKA, A. PENCIK, F. ALMQVIST, K. LJUNG, O. NOVAK a S. ROBERT. A role for the auxin precursor anthranilic acid in root gravitropism via regulation of PIN-FORMED protein polarity and relocalisation in Arabidopsis. \textit{New Phytologist}. HOBOKEN: Blackwell Science, 2019, roč.~223, č.~3, s.~1420-1432. ISSN~0028-646X. Dostupné z: https://dx.doi.org/10.1111/nph.15877.

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