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@article{1636928,
author = {Vaneckova, T. and Bezdekova, J. and Tvrdoňová, Michaela and Vlcnovska, M. and Novotna, V. and Neuman, J. and Štossová, Aneta and Kanický, Viktor and Adam, V. and Vaculovicova, M. and Vaculovič, Tomáš},
article_location = {LONDON},
article_number = {AUG},
doi = {http://dx.doi.org/10.1038/s41598-019-48290-2},
keywords = {MAGNETIC NANOPARTICLES; SENSITIVE DETECTION; SURFACE-CHEMISTRY; RECOGNITION; SENSOR; EXTRACTION; SEPARATION; DNA},
language = {eng},
issn = {2045-2322},
journal = {Scientific reports},
title = {CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection},
url = {https://www.nature.com/articles/s41598-019-48290-2#Ack1},
volume = {9},
year = {2019}
}
TY - JOUR
ID - 1636928
AU - Vaneckova, T. - Bezdekova, J. - Tvrdoňová, Michaela - Vlcnovska, M. - Novotna, V. - Neuman, J. - Štossová, Aneta - Kanický, Viktor - Adam, V. - Vaculovicova, M. - Vaculovič, Tomáš
PY - 2019
TI - CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection
JF - Scientific reports
VL - 9
IS - AUG
SP - 1-9
EP - 1-9
PB - NATURE PUBLISHING GROUP
SN - 20452322
KW - MAGNETIC NANOPARTICLES
KW - SENSITIVE DETECTION
KW - SURFACE-CHEMISTRY
KW - RECOGNITION
KW - SENSOR
KW - EXTRACTION
KW - SEPARATION
KW - DNA
UR - https://www.nature.com/articles/s41598-019-48290-2#Ack1
L2 - https://www.nature.com/articles/s41598-019-48290-2#Ack1
N2 - For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.
ER -
VANECKOVA, T., J. BEZDEKOVA, Michaela TVRDOŇOVÁ, M. VLCNOVSKA, V. NOVOTNA, J. NEUMAN, Aneta ŠTOSSOVÁ, Viktor KANICKÝ, V. ADAM, M. VACULOVICOVA a Tomáš VACULOVIČ. CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2019, roč.~9, AUG, s.~1-9. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-019-48290-2.
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