Další formáty:
BibTeX
LaTeX
RIS
@article{1639956,
author = {Pípal, Marek and Priebojová, Jana and Kočí, Tereza and Bláhová, Lucie and Smutná, Marie and Hilscherová, Klára},
article_location = {Oxford},
article_number = {FEB 2020},
doi = {http://dx.doi.org/10.1016/j.chemosphere.2019.125061},
keywords = {Retinoids; All-trans retinoic acid; Zebrafish; Teratogenicity; Retinoid-like activity; Cyanobacteria},
language = {eng},
issn = {0045-6535},
journal = {Chemosphere},
title = {Field cyanobacterial blooms producing retinoid compounds cause teratogenicity in zebrafish embryos},
url = {https://doi.org/10.1016/j.chemosphere.2019.125061},
volume = {241},
year = {2020}
}
TY - JOUR
ID - 1639956
AU - Pípal, Marek - Priebojová, Jana - Kočí, Tereza - Bláhová, Lucie - Smutná, Marie - Hilscherová, Klára
PY - 2020
TI - Field cyanobacterial blooms producing retinoid compounds cause teratogenicity in zebrafish embryos
JF - Chemosphere
VL - 241
IS - FEB 2020
SP - 1-14
EP - 1-14
PB - Pergamon-Elsevier Science Ltd
SN - 00456535
KW - Retinoids
KW - All-trans retinoic acid
KW - Zebrafish
KW - Teratogenicity
KW - Retinoid-like activity
KW - Cyanobacteria
UR - https://doi.org/10.1016/j.chemosphere.2019.125061
L2 - https://doi.org/10.1016/j.chemosphere.2019.125061
N2 - Cyanobacteria routinely release potentially harmful bioactive compounds into the aquatic environment. Several recent studies suggested a potential link between the teratogenicity of effects caused by cyanobacteria and production of retinoids. To investigate this relationship, we analysed the teratogenicity of field-collected cyanobacterial bloom samples by means of an in vivo zebrafish embryo test, an in vitro reporter gene bioassay and by the chemical analysis of retinoids. Extracts of biomass from cyanobacterial blooms with the dominance of Microcystis aeruginosa and Aphanizomenon klebahnii were collected from water bodies in the Czech Republic and showed significant retinoid-like activity in vitro, as well as high degrees of teratogenicity in vivo. Chemical analysis was then used to identify a set of retinoids in ng per gram of dry weight concentration range. Subsequent fractionation and bioassay-based characterization identified two fractions with significant in vitro retinoid-like activity. Moreover, in most of the retinoids eluted from these fractions, teratogenicity with malformations typical for retinoid signalling disruption was observed in zebrafish embryos after exposure to the total extracts and these in vitro effective fractions. The zebrafish embryo test proved to be a sensitive toxicity indicator of the biomass extracts, as the teratogenic effects occurred at even lower concentrations than those expected from the activity detected in vitro. In fact, teratogenicity with retinoid-like activity was detected at concentrations that are commonly found in biomasses and even in bulk water surrounding cyanobacterial blooms. Overall, these results provide evidence of a link between retinoid-like activity, teratogenicity and the retinoids produced by cyanobacterial water blooms in the surrounding environment.
ER -
PÍPAL, Marek, Jana PRIEBOJOVÁ, Tereza KOČÍ, Lucie BLÁHOVÁ, Marie SMUTNÁ a Klára HILSCHEROVÁ. Field cyanobacterial blooms producing retinoid compounds cause teratogenicity in zebrafish embryos. \textit{Chemosphere}. Oxford: Pergamon-Elsevier Science Ltd, 2020, roč.~241, FEB 2020, s.~1-14. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2019.125061.
|
